Analysis of the mechanism of trophoblast infiltration

Thymidylate synthase plays a central role in the biosynthesis of thymidylate, an essential precursor for DNA biosynthesis. In addition to its role in catalysis and cellular metabolism, it is now appreciated that thymidylate synthase functions as an RNA binding protein. Specifically, thymidylate synthase binds with high affinity to its own mRNA, resulting in translational repression. An extensive series of experiments has been performed to elucidate the molecular elements underlying the interaction between thymidylate synthase and its own mRNA. In addition to characterization of the underlying cis- and trans-acting elements, recent studies have shown that thymidylate synthase has the capacity to bind specifically to other cellular RNA species. While the biological significance of these other RNA/thymidylate synthase interactions remains to be defined, this work suggests a potential role for TS in coordinately regulating several critical aspects of cellular metabolism.     

Prefabricated osteomusculocutaneous flaps using calvarial bone: experimental study

Prefabricated osteomusculocutaneous flaps using free calvarial bone were examined and evaluated in a rat model. The animals were divided into two groups according to prefabrication time: 14 days in Group 1 (n = 10) and 28 days in Group 2 (n = 10). Nine of 10 preparations demonstrated neovascularization in Group 1, and all flaps showed neovascularization in Group 2. One flap was lost in Group 1 as a result of infection. Each group was evaluated histopathologically before the second stage of the experiment. Muscles without atrophy and osteocytes were noted in Group 1; however, Group 2 animals had both muscle atrophy and nonviable bone. The prefabricated osteomusculocutaneous flaps were then transferred as both island and free flaps. Flap viability was assessed on postoperative day 7 by macroscopic observation. Although all flaps survived in the island-flap group, two flaps failed to survive due to technical error in the free-flap group. Neovascularization was clearly evident by 2 weeks in the osteomusculocutaneous flaps; after 4 weeks, complete atrophy of the muscle meant that the flaps could no longer be characterized as osteomusculocutaneous. Clinically, it might be possible to use the outer table alone, in which case both thin skin and bone would be desirable. This study may provide a model for this approach.     

Modeling of a power electronic converter for EMC in the conduction emission frequency band

With the advancement of high‐frequency switching devices, electromagnetic interferences (EMI) have become problems in power electronic converter designs. It is necessary for an electromagnetic compatibility (EMC) design to prospect and consider its possible EMI levels. This paper describes how to compute effects from a power converter to an object point to reduce conduction EMI noises effectively by an appropriate design. Modeling techniques for converter elements are discussed for a model in the conduction emission frequency band by the parameter tuning method and for line constants by an analytical derivation. Then a derived model is simulated for harmonic distributions of loop currents and their magnetic fields. © 2002 Scripta Technica, Electr Eng Jpn, 139(1): 44–50, 2002: DOI 10.1002/eej.1145     

Morphological estimation of total number of influenza A type virion spikes.

When purified and stored influenza A type virions were observed by the negative staining method in electron microscopy, we found disintegrated virions associated with a population of dispersed but clustered spikes. The total spike numbers of the clusters were morphologically counted, and their peak number (about 200) was consistent with the spike number measured by the molecular biology method, described in the other report.     

The complexity of some decision problems about two-dimensional array grammars

The complexity of several decision problems concerning two-dimensional isometric array grammars (IAG) is studied. Because of the two-dimensional and the “isometric” properties of IAG, many decision problems become very hard to solve even for regular array grammars (RAG), the lowest subclass of IAG in the Chomsky-like hierarchy. In this paper, it is shown that the membership problems for RAGs and for context-free array grammars (CFAG) are both NP-complete. The emptiness problem and the equivalence problem for RAGs are shown to be undecidable.     

Dispersion of Verdet constant in stress-birefringent silica fibre

The Faraday effect in stress-birefringent silica fibre is discussed theoretically and measured experimentally. In the red and infra-red spectral range, the Verdet constant is found to be VH = 2.0 × 10?35 v2 rad/A, where v is the optical frequency in hertz.     

13C NMR and Fourier transform infrared study of electrochemically prepared polythienylene film

The ¹³C NMR and Fourier transform infrared (FTIR) spectra of an electrochemically prepared polythienylene film have been obtained. The polythienylene film displays only two sharp absorption bands, at 124.8 and 136.5 ppm downfield from TMS (tetramethylsilane) in the ¹³C NMR, these being attributable to the carbon atoms in the β-position and the α-position, respectively, relative to the sulfur atom of poly(2,5-thienylene). The polythienylene film also shows a sharp absorption band at 789 cm^?1 in the IR, which is definitely ascribable to the C-H out-of-plane vibration of poly(2,5-thienylene). The polythienylene film will be shown to have a highly defined structure with a dominance of poly(2,5-thienylene).     

Application of rigid polyurethane integral-skin foams to thermal insulating unit cases for car air conditioners

Integral-skin foams of rigid polyurethane are sandwich structures consisting of a core layer of closed cells enclosed in rigid surface layers on both sides. We examined the layer composition of integral-skin foam with the objective of maximum flexural strength, and then studied possibilities of reconciling the strength and thermal insulating properties in housings for evaporators in car air conditioners; i.e., unit cases. This examination showed that the most practical density range (250 ≦ ρ_pall ≦ 500 kg/m³) provides vibratile resistance and thermal insulating properties. In actual car-running tests, a maximum 0.1 MPa stress was generated on unit cases with overall densities of 350 kg/m³, We found this to be 0.4% of the flexural strength of an integral-skin foam and 2% of the fatigue strength. In the forcible vibratile test, a stress of 0.5 to 1.0 MPa was generated at the resonance point of a unit case with 250 to 500 kg/m³ overall density. We found that these values are 2 to 5% of integral-skin foam's flexural strength and 10 to 25% of its fatigue strength. These values are of the same level as the conventional unit case made of polypropylene blended with talc. An integral-skin foam with an overall density of 250 kg/m³, nearly equal to half the weight of polypropylene, has the same level of resistance to vibration.