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排序方式: 共有296条查询结果,搜索用时 15 毫秒
281.
282.
Yutaro Kondo Susumu Suzuki Shoya Ono Mitsuo Goto Satoru Miyabe Tetsuya Ogawa Hiromi Tsuchida Hideaki Ito Taishi Takahara Akira Satou Toyonori Tsuzuki Kazuhiro Yoshikawa Ryuzo Ueda Toru Nagao 《International journal of molecular sciences》2022,23(8)
The expression of programmed death ligand-1 (PD-L1) is controlled by complex mechanisms. The elucidation of the molecular mechanisms of PD-L1 expression is important for the exploration of new insights into PD-1 blockade therapy. Detailed mechanisms of the in situ expression of PD-L1 in tissues of oral squamous cell carcinomas (OSCCs) have not yet been clarified. We examined the mechanisms of PD-L1 expression focusing on the phosphorylation of downstream molecules of epidermal growth factor (EGF) and interferon gamma (IFN-γ) signaling in vitro and in vivo by immunoblotting and multi-fluorescence immunohistochemistry (MF-IHC), respectively. The in vitro experiments demonstrated that PD-L1 expression in OSCC cell lines is upregulated by EGF via the EGF receptor (EGFR)/PI3K/AKT pathway, the EGFR/STAT1 pathway, and the EGFR/MEK/ERK pathway, and by IFN-γ via the JAK2/STAT1 pathway. MF-IHC demonstrated that STAT1 and EGFR phosphorylation was frequently shown in PD-L1-positive cases and STAT1 phosphorylation was correlated with lymphocyte infiltration and EGFR phosphorylation. Moreover, the phosphorylation pattern of the related molecules in PD-L1-positive cells differed among the cases investigated. These findings indicate that PD-L1 expression mechanisms differ depending on the tissue environment and suggest that the examination of the tissue environment and molecular alterations of cancer cells affecting PD-L1 expression make it necessary for each patient to choose the appropriate combination drugs for PD-1 blockade cancer treatment. 相似文献
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284.
Setsuko Komatsu Hisateru Yamaguchi Keisuke Hitachi Kunihiro Tsuchida Yuhi Kono Minoru Nishimura 《International journal of molecular sciences》2021,22(16)
To investigate the mechanism of flooding tolerance of soybean, flooding-tolerant mutants derived from gamma-ray irradiated soybean were crossed with parent cultivar Enrei for removal of other factors besides the genes related to flooding tolerance in primary generated mutant soybean. Although the growth of the wild type was significantly suppressed by flooding compared with the non-flooding condition, that of the mutant lines was better than that of the wild type even if it was treated with flooding. A two-day-old mutant line was subjected to flooding for 2 days and proteins were analyzed using a gel-free/label-free proteomic technique. Oppositely changed proteins in abundance between the wild type and mutant line under flooding stress were associated in endoplasmic reticulum according to gene-ontology categorization. Immunoblot analysis confirmed that calnexin accumulation increased in both the wild type and mutant line; however, calreticulin accumulated in only the mutant line under flooding stress. Furthermore, although glycoproteins in the wild type decreased by flooding compared with the non-flooding condition, those in the mutant line increased even if it was under flooding stress. Alcohol dehydrogenase accumulated in the wild type and mutant line; however, this enzyme activity significantly increased and mildly increased in the wild type and mutant line, respectively, under flooding stress compared with the non-flooding condition. Cell death increased and decreased in the wild type and mutant line, respectively, by flooding stress. These results suggest that the regulation of cell death through the fermentation system and glycoprotein folding might be an important factor for the acquisition of flooding tolerance in mutant soybean. 相似文献
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286.
This paper describes a new type high torque induction motor which has the rotating magnets in the rotor. The motor basically consists of a usual stator, cylindrical rotor, and inner cylinder of which the surface is covered by a set of magnets. The rotor turns at somewhat less than synchronous speed. The inner cylinder with magnets can revolve freely against a rotor shaft. The magnets revolve synchronized by the rotating magnetic field induced by the stator current. The magnets make the flux in the rotor. Then we can expect torque increase by the increase of the flux. The results of magnetic field analysis indicate the flux is increased. In the experimental results of a test motor which is a 400 W prototype machine, we have obtained the torque increase by approximately 20 percent as anticipated in the magnetic field analysis. Moreover, test results show improvements of efficiency and power factor in the motor operations. The efficiency of the test motor is obtained as high as 10 percent at the rated output over those of a same size conventional induction motor. Although power factor of conventional induction motors is lagging at all times, the test motor can be operated with near unity, leading or lagging by adjusting the ac supplied source voltage. 相似文献
287.
288.
The separation of antimony and bismuth in copper electrorefining is somewhat difficult and costly. An ion-exchange technique
for the removal of the metals from the electrolyte has been practiced recently, although the metals recovered require further
processing. Sumitomo Metal Mining has developed a process to recover antimony and bismuth directly from the eluant by an electrowinning
process using a mixture of sulfuric acid and sodium chloride as an eluant for the desorption from the resin. The content of
bismuth in the antimony metal recovered by this process was less than 0.2%, while the content of antimony in the bismuth metal
was less than 0.4%.
K. Ando earned his M.Sc. in metallurgical engineering at Kumamoto University in 1986. He is currently a senior scientist at Sumitomo
Metal Mining Company.
N. Tsuchida earned his Ph.D. in mineral science at Murdoch University, Western Australia, in 1986. He is currently manager of the Niihama
nickel refinery at Sumitomo Metal Mining Company. 相似文献
289.
290.
Ohnishi T. Doi S. Tsuchida Y. Noguchi T. 《Electron Devices, IEEE Transactions on》1997,44(8):1253-1257
Highly luminous polymers have successfully been prepared by copolymerization of various arylene vinylene units. The random copolymerization of the monomers having different energy gaps or conjugation degrees can generate the irregularity in poly(arylene vinylenes) and enhance the fluorescence of the polymer. Among the copolymers synthesized, the m/p-phenylene vinylene copolymer showed fairly strong fluorescence and gave the most efficient polymer light-emitting diode (P-LED) device because of the good balance of the exciton confinement and the charge transporting property. The optimized P-LED device showed a maximum luminance of 70000 cd/m2 相似文献