Periodic Exposure of Plasma-Activated Medium Alters Fibroblast Cellular Homoeostasis

Excess amounts of redox stress and failure to regulate homeostatic levels of reactive species are associated with several skin pathophysiologic conditions. Nonmalignant cells are assumed to cope better with higher reactive oxygen and nitrogen species (RONS) levels. However, the effect of periodic stress on this balance has not been investigated in fibroblasts in the field of plasma medicine. In this study, we aimed to investigate intrinsic changes with respect to cellular proliferation, cell cycle, and ability to neutralize the redox stress inside fibroblast cells following periodic redox stress in vitro. Soft jet plasma with air as feeding gas was used to generate plasma-activated medium (PAM) for inducing redox stress conditions. We assessed cellular viability, energetics, and cell cycle machinery under oxidative stress conditions at weeks 3, 6, 9, and 12. Fibroblasts retained their usual physiological properties until 6 weeks. Fibroblasts failed to overcome the redox stress induced by periodic PAM exposure after 6 weeks, indicating its threshold potential. Periodic stress above the threshold level led to alterations in fibroblast cellular processes. These include consistent increases in apoptosis, while RONS accumulation and cell cycle arrest were observed at the final stages. Currently, the use of NTP in clinical settings is limited due to a lack of knowledge about fibroblasts’ behavior in wound healing, scar formation, and other fibrotic disorders. Understanding fibroblasts’ physiology could help to utilize nonthermal plasma in redox-related skin diseases. Furthermore, these results provide new information about the threshold capacity of fibroblasts and an insight into the adaptation mechanism against periodic oxidative stress conditions in fibroblasts.     

Typed Abstract State Machines for data-intensive applications

This paper focuses on extensions of Abstract State Machines (ASMs) for the benefit of a refinement calculus in the area of data-intensive applications, in particular data warehouses and on-line analytical processing. The extensions lead to a typed version of ASMs and a more specific notion of strong data refinement, which incorporates the preservation of information content by means of schema dominance. Each typed ASM can be effectively translated into an equivalent ordinary one. Providing typed ASMs helps to exploit the existing logical formalisms used in data-intensive applications to define a ground model. Furthermore, strong data refinement helps to set up a refinement-based development method that uses provably correct standard refinement rules.     

Quantitative Determination of HD Internal State Distributions via (2+1) REMPI

The relationship between quantum state populations and ion signals in (2+1) resonance-enhanced multiphoton ionization (REMPI) detection of HD is investigated both experimentally, by measurements of hot-nozzle population distributions, and theoretically, by calculations of the two-photon E,F ¹Σ_g⁺—X¹Σ_g⁺ transition moments. The experimental and theoretical relative rotational cross-sections are in good agreement for v″ = 0 and v″ = 1, but differ for v″ = 2 and for the relative vibrational cross-sections. Arguments are presented that saturation of the E,F-X transition is the main cause of these discrepancies. In spite of the presence of saturation, this calibration gives confidence in determining the relative internal state populations of HD from its (2+1) REMPI spectrum.     

Axiomatisations of functional dependencies in the presence of records,lists, sets and multisets

We investigate functional dependencies in databases that support complex values such as records, lists, sets and multisets. Therefore, an abstract algebraic framework is proposed that classifies data models according to the underlying types they support. This allows to emphasise the impact of the data types rather than the specifics of a particular data model.     

Delaminationsbruch von warmgewalztem,mikrolegiertem Baustahl

Beschreibung der Brucherscheinung. Gefügeuntersuchung an einem mikrolegierten Baustahl mit rd. 0,05% Nb und 0,1% V nach unterschiedlicher thermomechanischer Behandlung zur Ermittlung der Bruchursachen. Deutung der Bruchursachen durch Ausscheidungsvorgänge bei der thermomechanischen Behandlung.     

On-line monitoring and identification of bioaerosols

Fast analysis of bioaerosols in clean room environments is necessary in order to prevent contamination of pharmaceutical products, minimize machine downtimes, or both. The detection and identification of microbes will be carried out in several steps: After impaction of the aerosol on a surface, the particles are presorted with glancing light illumination and fluorescence imaging in order to distinguish between abiotic and biotic particles. Since only the biotic particles are of interest, the analysis time can be minimized due to reduction of the data set. The biotic particles are then analyzed further with Raman spectroscopy and identified with a support vector machine.