Antimicrobial interactions of microbial species involved in the fermentation of cassava dough into agbelima with particular reference to the inhibitory effect of lactic acid bacteria on enteric pathogens

Lactic acid bacteria, Bacillus species and yeasts are involved in the fermentation of cassava dough into agbelima. Microbial interactions within and between these groups of microorganisms were investigated in addition to the survival of five enteric pathogens inoculated into agbelima under various conditions. Nine out of 10 cultures of lactic acid bacteria isolated at the end of agbelima fermentation showed inhibitory effect against 10 cultures of lactic acid bacteria isolated at the start of fermentation. Only 3 out of 10 isolates of Bacillus subtilis were inhibited by 10 isolates of lactic acid bacteria tested. No interactions were observed between yeasts and the lactic acid bacteria, whereas three of the Bacillus isolates showed inhibitory effects against the yeasts. Twelve isolates of Lactobacillus plantarum tested inhibited the growth of an isolate each of Lactobacillus fermentum and Lactobacillus brevis but none tested positive for bacteriocin production. The antimicrobial effect of the lactic acid bacteria was attributed to acid production. In fermenting cassava dough, enteric pathogens survived to different extents depending on pH and their sensitivity to acids. Vibrio cholerae C-230, Salmonella typhimurium 9 and Salmonella enteritidis 226 were not detectable in 10 g of sample after 4 h when inoculated into the 48-h fermented product, agbelima, whereas Shigella dysenteriae 2357T and Escherichia coli D2188 were detectable up to 8 h in the product.     

Moisture-pressure combination treatments for cyanide reduction in grated cassava

Several cyanide-associated health disorders have been linked with frequent consumption of mildly toxic cassava (Manihot esculenta crantz) products in individuals on a low-protein diet. Production of bread from cassava often involves application of prolonged physical pressure (pressing) to the freshly grated root for several hours. This study aimed to determine effects of pressure and wetting on grated cassava. Six treatments were applied: confining pressure for 12 h, wetting for 4 h at 25 °C, 2 h at 25 °C, 2 h at 40 °C, and 2 h at 50 °C, or each of the above followed by pressure for 12 h. Treatments released cyanide from samples in the order: 2-h wet at 50 °C + pressing >4-h wet at 25 °C + pressing = 2-h wet at 40 °C + pressing >2-h wet at 25 °C + pressing = 4-h wet at 25 °C >12-h pressing. Wetting for 2 h at 50 °C followed by pressure for 12 h reduced cyanide levels by at least 20% more than that of any other treatment. The combination of moisture and pressure enhanced the contact time between linamarin and linamarase to increase the release of hydrogen cyanide.     

Residues of organochlorine pesticides in vegetables marketed in Greater Accra Region of Ghana

Residual levels of organochlorine pesticides (OCPs) were determined in 240 samples of vegetables collected from selected markets from Greater Accra region of Ghana in July 2010 to February 2011. The determination was done using gas chromatography with electron capture detector (GC-ECD). The compounds targeted were lindane, heptachlor + its epoxide, endrin, dieldrin, o,p′-DDE, p,p′-DDE, o,p′-DDD, o,p′-DDT and p,p′-DDT. The results indicated that all the vegetables sampled had some levels of one or more OCPs in them. Residues of pesticides were found in 71.9% of all the vegetable samples analyzed indicating high incidence of these xenobiotics in the vegetables from the markets and 31.48% samples were above the maximum residue levels (MRLs). The most frequently found and abundant pesticides were the metabolites of DDT (o,p′-DDE, p,p′-DDE and o,p′-DDD), followed by lindane and then o,p′-DDT. The residue levels and the detection rate of the OCPs indicate that, vegetables from supermarket had higher OCPs levels, followed by roadside grocery stores and open markets. The results recommend the need for regular monitoring of a greater number of samples for long periods for pesticide residues especially in fruits and vegetables to protect consumers’ health.     

Complex coordinate implementation in the curvilinear coordinate method: application to plane-wave diffraction by nonperiodic rough surfaces

We investigate the electromagnetic modeling of plane-wave diffraction by nonperiodic surfaces by using the curvilinear coordinate method (CCM). This method is often used with a Fourier basis expansion, which results in the periodization of both the geometry and the electromagnetic field. We write the CCM in a complex coordinate system in order to introduce the perfectly matched layer concept in a simple and efficient way. The results, presented for a perfectly conducting surface, show the efficiency of the model.     

Case Study: Hydraulic Modeling of Runoff Processes in Ghanaian Inland Valleys

The inland valleys of West Africa are strategic in terms of food security and poverty alleviation, but scientific studies on hydrologic processes happening in these environments have not been well documented. Modeling approaches presented in this paper are an attempt to better comprehend hydraulic phenomena occurring in inland valleys. An inland valley situated in the Northern Region of Ghana is set as the study site. The inland valley comprises well-drained uplands and hydromorphic valley bottoms. There are several earthen dams across the valley bottoms, which are at the same time seasonal wetlands cultivated to rice during the rainy season. A finite volume model for the shallow water equations is developed to numerically simulate surface runoff flows in the valley bottoms during flood events. Innovation is necessitated to handle a series of different hydraulic phenomena. Flux-splitting and data reconstruction techniques are used to achieve stable computation in the complex topography of the valley bottoms. Standard problems of oblique hydraulic jump and dam break flows are used to test the accuracy of the numerical model. The Manning’s roughness coefficient is determined from calibration in another Ghanaian watershed located in the Eastern Region. Using actually observed time series data of rainfall intensity, surface flows during the rainfall events are simulated in the computational domain representing the valley bottoms of the study area. Observed data of water levels in the dams are compared to predictions, and discrepancies between them are examined from the hydrological point of view. In the case of a hypothetical flood event, cascading collapses of the dams and flooding of cultivated fields are reproduced.     

Diverse Sphingolipid Species Harbor Different Effects on Ire1 Clustering

Endoplasmic reticulum (ER) function is dedicated to multiple essential processes in eukaryotes, including the processing of secretory proteins and the biogenesis of most membrane lipids. These roles implicate a heavy burden to the organelle, and it is thus prone to fluctuations in the homeostasis of molecules which govern these processes. The unfolded protein response (UPR) is a general ER stress response tasked with maintaining the ER for optimal function, mediated by the master activator Ire1. Ire1 is an ER transmembrane protein that initiates the UPR, forming characteristic oligomers in response to irregularities in luminal protein folding and in the membrane lipid environment. The role of lipids in regulating the UPR remains relatively obscure; however, recent research has revealed a potent role for sphingolipids in its activity. Here, we identify a major role for the oxysterol-binding protein Kes1, whose activity is of consequence to the sphingolipid profile in cells resulting in an inhibition of UPR activity. Using an mCherry-tagged derivative of Ire1, we observe that this occurs due to inhibition of Ire1 to form oligomers. Furthermore, we identify that a sphingolipid presence is required for Ire1 activity, and that specific sphingolipid profiles are of major consequence to Ire1 function. In addition, we highlight cases where Ire1 oligomerization is absent despite an active UPR, revealing a potential mechanism for UPR induction where Ire1 oligomerization is not necessary. This work provides a basis for the role of sphingolipids in controlling the UPR, where their metabolism harbors a crucial role in regulating its onset.     

Espoused cultural values as antecedents of individuals’ threat and coping appraisal toward protective information technologies: Study of U.S. and Ghana

Evidence of cyber activities in developing nations has renewed discourse on users’ intention to protect information in the global economy. We present a context theorization in which espoused individualism–collectivism and uncertainty avoidance are posited as antecedents to an individual’s threat and coping appraisal toward protecting information. We tested our model with data from the United States and Ghana, countries that generally differ on cultural values. Individualism–collectivism and uncertainty avoidance significantly affect threat and coping appraisals with uncertainty avoidance demonstrating slightly stronger effect. Path estimate comparisons between Ghana and the United States show significant differences for all of the structural paths.     

Cellular and Exosomal Regulations of Sepsis-Induced Metabolic Alterations

Sepsis is a sustained systemic inflammatory condition involving multiple organ failures caused by dysregulated immune response to infections. Sepsis induces substantial changes in energy demands at the cellular level leading to metabolic reprogramming in immune cells and stromal cells. Although sepsis-associated organ dysfunction and mortality have been partly attributed to the initial acute hyperinflammation and immunosuppression precipitated by a dysfunction in innate and adaptive immune responses, the late mortality due to metabolic dysfunction and immune paralysis currently represent the major problem in clinics. It is becoming increasingly recognized that intertissue and/or intercellular metabolic crosstalk via endocrine factors modulates maintenance of homeostasis, and pathological events in sepsis and other inflammatory diseases. Exosomes have emerged as a novel means of intercellular communication in the regulation of cellular metabolism, owing to their capacity to transfer bioactive payloads such as proteins, lipids, and nucleic acids to their target cells. Recent evidence demonstrates transfer of intact metabolic intermediates from cancer-associated fibroblasts via exosomes to modify metabolic signaling in recipient cells and promote cancer progression. Here, we review the metabolic regulation of endothelial cells and immune cells in sepsis and highlight the role of exosomes as mediators of cellular metabolic signaling in sepsis.     

Effects of admission and treatment strategies of DWI courts on offender outcomes

Purpose

The purpose of this study is to classify DWI courts on the basis of the mix of difficult cases participating in the court (casemix severity) and the amount of involvement between the court and participant (service intensity). Using our classification typology, we assessed how casemix severity and service intensity are associated with program outcomes. We expected that holding other factors constant, greater service intensity would improve program outcomes while a relatively severe casemix would result in worse program outcomes.

Methods

The study used data from 8 DWI courts, 7 from Michigan and 1 from North Carolina. Using a 2-way classification system based on court casemix severity and program intensity, we selected participants in 1 of the courts, and alternatively 2 courts as reference groups. Reference group courts had relatively severe casemixes and high service intensity. We used propensity score matching to match participants in the other courts to participants in the reference group court programs. Program outcome measures were the probabilities of participants’: failing to complete the court's program; increasing educational attainment; participants improving employment from time of program enrollment; and re-arrest.

Results

For most outcomes, our main finding was that higher service intensity is associated with better outcomes for court participants, as anticipated, but a court's casemix severity was unrelated to study outcomes.

Conclusions

Our results imply that devoting more resources to increasing duration of treatment is productive in terms of better outcomes, irrespective of the mix of participants in the court's program.     

Souring and breakdown of cyanogenic glucosides during the processing of cassava into akyeke

The population and composition of the lactic acid bacteria microbiota as well as the content of cyanogenic glucosides occurring at various stages of fermentation and subsequent processing of cassava roots into akyeke, a steamed sour cassava meal, were investigated. The number of lactic acid bacteria and percentage titratable acidity increased during 5 days of fermentation, but decreases were observed in the subsequent operations of 'washing' the dough with water followed by partial drying and steaming. In field and laboratory samples, Lactobacillus plantarum accounted for 59.3% and 52.3%, Lactobacillus brevis 23.3% and 22.8% and Leuconostoc mesenteroides subsp. cremoris 14.5% and 15.8%, respectively, of all lactic acid bacteria isolated at various stages of fermentation and processing. A reduction of about 98% occurred in the total cyanogens (CN) content of cassava roots during processing, from 69.3 to 1.4 and 110.3 to 2.8 mg CN equivalent/kg dry weight for laboratory and field samples of akyeke, respectively.