Effects of computer-assisted instruction on performance of senior high school biology students in Ghana

This study investigated the comparative efficiency of computer-assisted instruction (CAI) and conventional teaching method in biology on senior high school students. A science class was selected in each of two randomly selected schools. The pretest-posttest non equivalent quasi experimental design was used. The students in the experimental group learned science concepts (cell cycle) through the CAI, whereas the students in the control group were taught the same concepts by the conventional approach. The conventional approach consisted of lecture, discussions and question and answer teaching methods. Mann–Whitney U tests were used to analyze students’ pretest and posttests scores. The results indicated that students that were instructed by the conventional approach performed better on the posttest than those instructed by the CAI. However, the performance of low achievers within the experimental group improved after they were instructed by the CAI. Even though the CAI group did not perform better than the conventional approach group, the students in the CAI group perceived CAI to be interesting when they were interviewed.     

Supplementing urban water supply with rainwater harvesting in Accra,Ghana

This article examines the challenges associated with rainwater harvesting and usage in peri-urban Accra. Data collected from 357 heads of household reveal that rainwater harvesting has the potential to supplement existing water sources in peri-urban Accra. However, high investment costs for rainwater harvesting facilities, short-term tenancy arrangements, the perception that rainwater is not clean, and the unique dry climate of the Accra Plains emerge as key challenges limiting domestic use of rainwater. Public education for house owners to invest in rainwater harvesting facilities and governmental support will be needed to increase investment in rainwater harvesting, purification and usage.     

n-3 Docosapentaenoic Acid Intake and Relationship with Plasma Long-Chain n-3 Fatty Acid Concentrations in the United States: NHANES 2003–2014

The long-chain n-3 fatty acids, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), play a crucial role in health, but previous National Health and Nutrition Examination Survey (NHANES) analyses have shown that EPA and DHA intake in the United States is far below recommendations (~250–500 mg/day EPA + DHA). Less is known about docosapentaenoic acid (DPA), the metabolic intermediate of EPA and DHA; however, evidence suggests DPA may be an important contributor to long-chain n-3 fatty acid intake and impart unique benefits. We used NHANES 2003–2014 data (n = 45,347) to assess DPA intake and plasma concentrations, as well as the relationship between intake and plasma concentrations of EPA, DPA, and DHA. Mean DPA intake was 22.3 ± 0.8 mg/day from 2013 to 2014, and increased significantly over time (p < 0.001), with the lowest values from 2003 to 2004 (16.2 ± 1.2 mg/day). DPA intake was higher in adults (20–55 years) and seniors (55+ years) compared to younger individuals. In regression analyses, DPA intake was a significant predictor of plasma EPA (β = 138.5; p < 0.001) and DHA (β = 318.9; p < 0.001). Plasma DPA was predicted by EPA and DHA intake (β = 13.15; p = 0.001 and β = 7.4; p = 0.002), but not dietary DPA (p = 0.3). This indicates that DPA intake is not a good marker of plasma DPA status (or vice versa), and further research is needed to understand the factors that affect the interconversion of EPA and DPA. These findings have implications for future long-chain n-3 fatty acids dietary recommendations.     

Modal method based on subsectional Gegenbauer polynomial expansion for lamellar gratings

A first approach of a modal method by Gegenbauer polynomial expansion (MMGE1) is presented for a plane wave diffraction by a lamellar grating. Modal methods are among the most popular methods that are used to solve the problem of lamellar gratings. They consist in describing the electromagnetic field in terms of eigenfunctions and eigenvalues of an operator. In the particular case of the Fourier modal method (FMM), the eigenfunctions are approximated by a finite Fourier sum, and this approximation can lead to a poor convergence of the FMM. The Wilbraham-Gibbs phenomenon may be one of the reasons for this poor convergence. Thus, it is interesting to investigate other basis functions that may represent the fields more accurately. The approach proposed in this paper consists in subdividing the pattern in homogeneous layers, according to the periodicity axis. The field is expanded, in each layer, on the basis of Gegenbauer's polynomials. Boundary conditions are rigorously written between adjacent layers; thus, an eigenvalue equation is obtained. The approach presented in this paper proves to describe the fields accurately. Finally, it is demonstrated that the results obtained with the MMGE1 are more accurate than several existing modal methods, such as the classical and the parametric FMM.     

Characterization of a 12 kDa thermal-stable antigenic protein in bovine blood

We have previously developed an immunoassay based on monoclonal antibody (MAb) Bb1H9 for quantitative detection of ruminant blood in processed food and feedstuffs. The purpose of this study was to characterize the unknown 12 kDa thermal-stable ruminant-specific antigenic protein recognized by MAb Bb1H9 in order to better define the application scope of the developed assay. Extracts obtained from raw and heat-treated bovine blood-derived products were analyzed with indirect ELISA and Western blot. Target proteins resolved by 2D electrophoresis were subjected to N-terminal sequencing. Results indicated that the 12 kDa protein is a monomer of the tetrameric hemoglobin molecule (64.5 kDa) and that the heme group is not required for its binding with MAb Bb1H9. This MAb can be utilized as a probe for red blood cell derived products of ruminant origin in raw or processed food and feedstuffs to enforce labeling regulations and to address consumer concerns. PRACTICAL APPLICATION: MAb Bb1H9 represents the first antibody with the capacity to recognize bovine hemoglobin both in the absence and presence of the heme group, regardless of the heat treatment. MAb Bb1H9 can therefore be utilized in immunoassays by manufacturers and regulators to detect any ingredients containing hemoglobin or globin (hemoglobin without the heme group) in both raw and processed food and feed materials for product quality control and labeling law enforcement.     

A preliminary assessment of consumer’s exposure to organochlorine pesticides in fruits and vegetables and the potential health risk in Accra Metropolis,Ghana

Accra Metropolis is the major recipient of agricultural produce in Ghana, with almost the entire population depending on rural agricultural activities for subsistence. To increase agricultural produce, various forms of pesticides are normally used in fruits and vegetable farming. The consequence is that several cases of pesticide poisoning have been observed. In this preliminary study, a field survey was carried out to assess the knowledge, attitudes, and peoples’ perception concerning the use of pesticides by farmers in fruit production. The residual concentrations of selected pesticides in pawpaw, imported apples and tomato samples and the potential health risks associated with the exposure to these pesticides were also assessed. The field survey data indicated that more than 70% of the participants reported one or more poisoning upon fruits consumption resulting in pesticide-related illnesses. Pesticide residues in the various environmental samples were determined by gas chromatography with electron capture detector (GC-ECD). The results showed that, the levels of pesticide residues varied from <0.01 μg/g to as high as 0.11 μg/g depending on the pesticide monitored. The analysis of health risk estimates revealed heptachlor, heptachlor epoxide, endrin aldehyde, and endrin ketone levels exceeded the reference dose, suggesting a great potential for systemic toxicity in children considered the most vulnerable population subgroup. Lastly, the public is in the know of pesticide residues in fruits and calls for the curtailing of pesticide use on fruits and vegetable production via educational programmes for farmers on control and safer use of pesticides.