Strain variability of the behavior of foodborne bacterial pathogens: A review

Differences in phenotypic responses among strains of the same microbial species constitute an important source of variability in microbiological studies, and as such they need to be assessed, characterized and taken into account. This review provides a compilation of available research data on the strain variability of four basic behavioral aspects of foodborne bacterial pathogens including: (i) virulence; (ii) growth; (iii) inactivation; and (iv) biofilm formation. A particular emphasis is placed on the foodborne pathogens Listeria monocytogenes and Salmonella enterica. The implications of strain variability for food safety challenge studies and microbial risk assessment are discussed also. The information provided indicates that the variability among strains of foodborne bacterial pathogens with respect to their behavior can be significant and should not be overlooked. However, in order for the mechanisms underlying the observed strain variability to be elucidated and understood, phenotypic variability data, such as those reviewed here, should be evaluated in conjunction with corresponding findings of studies assessing the molecular/physiological basis of this variability.     

Multifunctional materials: engineering applications and processing challenges

Multifunctional materials are designed so as to meet specific requirements through tailored properties. Smart materials can be considered as multifunctional ones that have the ability to react upon an external stimulus, simulating, in this way, the behavior of nature’s materials. Furthermore, the introduction of biomemetics in the material science, allows the designing of materials with similar processes as nature does: building from molecules to complete structures. This paper focuses on the presentation of the various multifunctional materials reported in the literature and the processing means developed.     

Numerical and analytical investigation of collapse loads of laced built-up columns

Built-up columns are often used in steel buildings and bridges providing economical solutions in cases of large spans and/or heavy loads. Two main effects should be taken into account in their design that differentiate them from other structural members. One is the significant influence of shear deformations due to their reduced shear rigidity. The second is the interaction between global and local buckling. These effects are addressed here from both a numerical and an analytical point of view for laced built-up columns. It is concluded that the largest loss of capacity occurs when the local and global Euler critical stresses and the yield stress all coincide. This reduction in capacity becomes more prominent in the presence of imperfections, reaching magnitudes in the order of 50%. Despite the detrimental effects of mode interaction many major design codes do not provide sufficient pertinent guidance. In order to address this issue, a simple analytical method is proposed for calculating the collapse load of laced built-up members taking into account the above effects as well as global imperfections, local out-of straightness and plasticity, which is then verified by means of nonlinear finite element analysis, using either beam or shell elements. The proposed method is found to provide improved accuracy in comparison to EC3 specifications in cases of global elastic failure.     

Towards Anonymous Mobile Community services

In the current market conditions, network operators are in search of novel value-added services that will increase their revenue. This paper introduces the innovative concept of Anonymous Mobile Community (AMC) services and thoroughly defines and describes a robust platform targeted for their deployment. AMC services take advantage of the terminals’ capabilities to collect information and deliver it to the network. In this context, terminals are enabled to form communities that serve as sources of information. In these communities, the anonymity and privacy of the end-users are respected and guarded. Several examples of promising AMC services are presented and categorized. An indicative example application is the provision of real-time information regarding road-traffic conditions, based on the location and speed of mobile terminals. A system aiming at the provision of diverse AMC services is proposed, and its requirements, architecture and functionality are described in detail. The related scalability issues and business models are carefully outlined, and a use case scenario as well as trial results are presented.     

Experimental assessment of the effects of cross-traffic on Wi-Fi video streaming

Wi-Fi networks are the first and sometimes only choice for the video streaming in homes, airports, malls, public areas and museums. However, Wi-Fi networks are vulnerable to interference, noise and have bandwidth limitations. Due to the intrinsic vulnerability of the communication channel, and the large number of variables involved, simulation alone is not enough in the evaluation of the performance of wireless networks. Actually, there is a tendency to give experimental tests a central role in the assessment of Wi-Fi networks performance.The paper presents an experimental analysis of the effects of cross traffic on the performance of video streaming over Wi-Fi, based on cross-layer measurements. Experiments are carried out in a semi-anechoic chamber, to prevent the results from being influenced by external factors. The experimental results permit to analyze the influence of cross traffic characteristics on cross layer measures and objective video quality metrics evaluated through a standardized approach.     

Interactions between hemoglobin and cod muscle constituents following treatment at extreme pH values

Acid and/or alkaline solubilization is a recent method developed to separate proteins from muscle foods with good functional properties. However, exposure of the muscle and its components to low pH values has been shown to promote lipid oxidation, limiting therefore the applications of this novel method. This research aimed primarily to study the physicochemical changes of the fish membranes brought about during acid or alkali solubilization processes. The effect on lipid oxidation and the possible role of the water soluble fraction of the muscle (press juice) as a potent antioxidant were also investigated. Model systems comprising minced cod muscle or cod microsomal suspensions were used. Results showed that acid or alkaline treatment (pH < 3.5 or pH > 10.5) of cod membranes significantly delayed lipid oxidation. Added triacylglycerols to washed cod system treated at low pH did not enhance hemoglobin-mediated lipid oxidation. Decreased precipitation of hemoglobin was observed with the alkali-treated membranes at all protein concentrations compared to the acid-treated and the untreated membranes. Finally, the addition of press juice to washed cod muscle tissue or to the membrane model system, significantly delayed hemoglobin lipid oxidation. PRACTICAL APPLICATION: The results of this study can be used to improve pH-shifting technologies to avoid or decrease lipid oxidation problems. Also, the use of press-juice from cod muscle as means of protecting the muscle against lipid oxidation is suggested.     

A Patterned 3D Silicon Anode Fabricated by Electrodeposition on a Virus‐Structured Current Collector

Electrochemical methods were developed for the deposition of nanosilicon onto a 3D virus‐structured nickel current collector. This nickel current collector is composed of self‐assembled nanowire‐like rods of genetically modified tobacco mosaic virus (TMV1cys), chemically coated in nickel to create a complex high surface area conductive substrate. The electrochemically depo­sited 3D silicon anodes demonstrate outstanding rate performance, cycling stability, and rate capability. Electrodeposition thus provides a unique means of fabricating silicon anode materials on complex substrates at low cost.     

Method of excess fractions with application to absolute distance metrology: theoretical analysis

The method of excess fractions (EF) is well established to resolve the fringe order ambiguity generated in interferometric detection. Despite this background, multiwavelength interferometric absolute long distance measurements have only been reported with varying degrees of success. In this paper we present a theoretical model that can predict the unambiguous measurement range in EF based on the selected measurement wavelengths and phase noise. It is shown that beat wavelength solutions are a subset of this theoretical model. The performance of EF, for a given phase noise, is shown to be equivalent to beat techniques but offers many alternative sets of measurement wavelengths and therefore EF offer significantly greater flexibility in experimental design.