Effects of polyunsaturated fatty acids and their n-6 hydroperoxides on growth of five malignant cell lines and the significance of culture media

We examined effects of polyunsaturated fatty acids (PUFA), their corresponding hydroperoxy fatty acids (hp-PUFA), as well as various pro- and antioxidants on the growth of tumor cells in culture. When cultured in RPMI 1640 medium, A-427 and WEHI clone 13 cells were both highly sensitive to hydroperoxy docosahexaenoic acid (hp-DHA), but they were far less sensitive in minimum essential medium (MEM). In contrast, A-427 cells were also sensitive to DHA in both culture media, while WEHI clone 13 cells, as well as other cell lines, tested in their respective media, were resistant. The lower sensitivity of the cell lines to hp-DHA in MEM-medium was apparently due to a more rapid reduction of hp-DHA to the corre-sponding hydroxy-DHA in MEM-medium. Addition of glutathione (GSH) to the culture medium abolished the effects of hp-DHA, but not the effects of DHA, while depletion of intracellular GSH levels by L-buthionine-S,R-sulfoximine strongly enhanced the cytotoxic effect of hp-DHA, but not the cytotoxic effect of DHA. α-Tocopherol protected A-427 cells against the toxic effect of DHA and abolished the induced lipid peroxidation, while it did not protect against the toxic effects of hp-DHA in A-427 or WEHI clone 13 cells. Ascorbic acid reduced the cytotoxic effect of DHA, but potentiated the toxic effect of hp-DHA while selenite essentially abolished the toxicity of both DHA and hp-DHA. These results indicate that sensitivity of tumor cell lines to PUFA and their oxidation products depends on their antioxidant defense mechanisms, as well as culture conditions, and establishes hp-DHA as a major, but probably not the sole, metabolite responsible for cytotoxicity of DHA.     

Sixth graders’ evaluation strategies when reading Internet search results: an eye-tracking study

Eye-tracking technology was used to examine Internet search result evaluation strategies adopted by sixth-grade students (N?=?36) during ten experimental information search tasks. The relevancy of the search result’s title, URL, and snippet components was manipulated and selection of search results as well as looking into probabilities on the search result components was analysed. The results revealed that during first-pass inspection, students read the search engine page by first looking at the title of a search result. If the title was relevant, the probability of looking at the snippet of the search result increased. During second-pass inspection, there was a high probability of students focusing on the most promising search result by inspecting all of its components before making their selection. A cluster analysis revealed three viewing strategies: half of the students looked mainly at the titles and snippets; one-third with high probability examined all components; and one-sixth mainly focused on titles, leading to more frequent errors in search result selection. The results indicate that students generally made a flexible use of both eliminative and confirmatory evaluation strategies when reading Internet search results, while some seemed to not pay attention to snippet and URL components of the search results.     

Gravitational drainage of compressible organic materials

A model was developed to simulate drainage of compressible particle suspensions, and study how cake compression and volumetric load influence the process. The input parameters were settling velocity, cake resistance and compressibility. These parameters were found using a new experimental method. Dextran‐MnO₂ particle suspensions were drained as these resemble organic waste slurries with respect to settling and compressibility. It was demonstrated that cake compressibility must be taken into account to obtain adequate simulations. This implies that pressurized filtration resistances cannot be used for drainage simulations. In the filtration step, a distinct increase of dry matter from top to bottom of the cake was observed. During the subsequent consolidation, the cake compressed and a uniform dry matter profile was found. The final dry matter content of the cake increased with feed concentration and volumetric load. The drainage time increased proportionally with feed concentration and, more importantly, proportionally with squared volumetric load. © 2010 American Institute of Chemical Engineers AIChE J, 2010     

Projecting demand and supply of forest biomass for heating in Norway

This paper assesses the increase in demand and supply for forest biomass for heating in Norway in 2020. By then there is a political aim to double the national production of bioenergy from the level in 2008. The competitiveness of woody biomass in central and district heating is analyzed in a model selecting the least-cost heating technology and scale in municipalities given a set of constraints and under different fuels price scenarios. The supply of forest biomass from roundwood is estimated based on data of forest inventories combined with elasticities regarding price and standing volumes. The supply of biomass from harvesting residues is estimated in an engineering approach based on data from the national forest inventories and roundwood harvest. The results show how the production of bioenergy is affected by changes in energy prices and support schemes for bioenergy. One conclusion from the analyses is that the government target of 14 TWh more bioenergy by 2020 is not likely to be met by current technologies and policy incentives. The contribution of the analysis is the detailed presentation of the heat market potentials and technology choices combined with supply functions for both roundwood and harvesting residues.     

Matrix models of discretely bending, stiff polymers

Polymer models which make use of the Ising model and transfer matrix techniques remind us, for example, of the work of Flory [Statistical mechanics of chain molecules, 1969] and Zimm and Bragg [J Chem Phys, 31 (1959) 526]. We investigate the properties of some such polymer models where the chain conformation can be described solely by an Ising-like parameterization and a set of independent, predetermined bond direction vectors or by a Potts-like model for directions of bond vectors on a lattice, with the specific aim of understanding more closely the connection of constraints and forces on the chain ends for polymers which, in general, are of arc length corresponding to their persistence lengths. Instances of these models are directed helical walks, random sequential walks, bimodally distributed in direction walks or relatively short, stiff chains fixed into a network. The behavior of this model under deformation in statistical mechanics and its dynamical properties under Glauber dynamics are discussed.     

Structure–Activity Relationship Study of Spider Polyamine Toxins as Inhibitors of Ionotropic Glutamate Receptors

The spider polyamine toxins Joro spider toxin‐3 (JSTX‐3) and Nephila polyamine toxins‐1 and ‐8 (NPTX‐1 and NPTX‐8) are isolated from the venom of the orb‐weaver spider Nephila clavata (Joro spider). They share a high degree of structural resemblance, their aromatic head groups being the only difference, and were recently found to be very potent open‐channel blockers of ionotropic glutamate (iGlu) receptors. In this study we designed and synthesized a collection of 24 analogues of these toxins using a recently developed solid‐phase synthetic methodology. Systematic variation in two regions of the toxins and subsequent evaluation of biological activity at AMPA and NMDA subtypes of iGlu receptors provided succinct information on structure–activity relationships. In particular, one set of analogues were found to display exquisite selectivity and potency for AMPA receptors relative to the natural products. Thus, this systematic SAR study has provided new pharmacological tools for studies of iGlu receptors.