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Dr. Laura Fourmois Dr. Florent Poyer Dr. Aude Sourdon Delphine Naud-Martin Dr. Sounderya Nagarajan Dr. Rahima Chennoufi Dr. Eric Deprez Dr. Marie-Paule Teulade-Fichou Dr. Florence Mahuteau-Betzer 《Chembiochem : a European journal of chemical biology》2021,22(14):2457-2467
Mitochondria are involved in many cellular pathways and dysfunctional mitochondria are linked to various diseases. Hence efforts have been made to design mitochondria-targeted fluorophores for monitoring the mitochondrial status. However, the factors that govern the mitochondria-targeted potential of dyes are not well-understood. In this context, we synthesized analogues of the TP-2Bzim probe belonging to the vinyltriphenylamine (TPA) class and already described for its capacity to bind nuclear DNA in fixed cells and mitochondria in live cells. These analogues ( TP-1Bzim, TPn-2Bzim, TP1+-2Bzim, TN-2Bzim ) differ in the cationic charge, the number of vinylbenzimidazolium branches and the nature of the triaryl core. Using microscopy, we demonstrated that the cationic derivatives accumulate in mitochondria but do not reach mtDNA. Under depolarisation of the mitochondrial membrane, TP-2Bzim and TP1+-2Bzim translocate to the nucleus in direct correlation with their strong DNA affinity. This reversible phenomenon emphasizes that these probes can be used to monitor ΔΨm variations. 相似文献
174.
Mnica Cicundez Laura Casarrubios María Jos Feito Iratxe Madarieta Nerea Garcia-Urkia Olatz Murua Beatriz Olalde Nerea Briz Rosalía Diez-Orejas María Teresa Portols 《International journal of molecular sciences》2021,22(8)
The decellularized extracellular matrix (ECM) obtained from human and porcine adipose tissue (AT) is currently used to prepare regenerative medicine bio-scaffolds. However, the influence of these natural biomaterials on host immune response is not yet deeply understood. Since macrophages play a key role in the inflammation/healing processes due to their high functional plasticity between M1 and M2 phenotypes, the evaluation of their response to decellularized ECM is mandatory. It is also necessary to analyze the immunocompetence of macrophages after contact with decellularized ECM materials to assess their functional role in a possible infection scenario. In this work, we studied the effect of four decellularized adipose matrices (DAMs) obtained from human and porcine AT by enzymatic or chemical methods on macrophage phenotypes and fungal phagocytosis. First, a thorough biochemical characterization of these biomaterials by quantification of remnant DNA, lipids, and proteins was performed, thus indicating the efficiency and reliability of both methods. The proteomic analysis evidenced that some proteins are differentially preserved depending on both the AT origin and the decellularization method employed. After exposure to the four DAMs, specific markers of M1 proinflammatory and M2 anti-inflammatory macrophages were analyzed. Porcine DAMs favor the M2 phenotype, independently of the decellularization method employed. Finally, a sensitive fungal phagocytosis assay allowed us to relate the macrophage phagocytosis capability with specific proteins differentially preserved in certain DAMs. The results obtained in this study highlight the close relationship between the ECM biochemical composition and the macrophage’s functional role. 相似文献
175.
Norgauer Philipp Grunewald Laura Priester Lena Heilemann Jens Hein Michael Stahl Karsten 《Forschung im Ingenieurwesen》2020,84(2):141-150
Forschung im Ingenieurwesen - Schneckengetriebe finden sich in verschiedenen Anwendungen als Stell- und Leistungsgetriebe. Aufgrund der Realisierbarkeit einer hohen Übersetzung bei kleinem... 相似文献
176.
Frida S. Herrera-Vázquez Dr. Félix Matadamas-Martínez Dr. Rodrigo Aguayo-Ortiz Prof. Laura Dominguez Teresa Ramírez-Apan Prof. Lilián Yépez-Mulia Prof. Francisco Hernández-Luis 《ChemMedChem》2020,15(19):1802-1812
Microtubules are highly dynamic polymers composed of α- and β-tubulin proteins that have been shown to be potential therapeutic targets for the development of anticancer drugs. Currently, a wide variety of chemically diverse agents that bind to β-tubulin have been reported. Nocodazole (NZ) and colchicine (COL) are well-known tubulin-depolymerizing agents that have close binding sites in the β-tubulin. In this study, we designed and synthesized a set of nine 2,4-diaminoquinazoline derivatives that could occupy both NZ and COL binding sites. The synthesized compounds were evaluated for their antiproliferative activities against five cancer cell lines (PC-3, HCT-15, MCF-7, MDA-MB-231, and SK-LU-1), a noncancerous one (COS-7), and peripheral blood mononuclear cells (PBMC). The effect of compounds 4 e and 4 i on tubulin organization and polymerization was analyzed on the SK-LU-1 cell line by indirect immunofluorescence, western blotting, and tubulin polymerization assays. Our results demonstrated that both compounds exert their antiproliferative activity by inhibiting tubulin polymerization. Finally, a possible binding pose of 4 i in the NZ/COL binding site was determined by using molecular docking and molecular dynamics (MD) approaches. To our knowledge, this is the first report of non-N-substituted 2,4-diaminoquinazoline derivatives with the ability to inhibit tubulin polymerization. 相似文献
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178.
Jordan P. Hooker Florian Feist Laura Delafresnaye Leonie Barner Christopher Barner‐Kowollik 《Advanced functional materials》2020,30(26)
A new strategy for particle synthesis is enabled by utilizing modern synthetic, polymer, and photochemical techniques to facilitate the synthesis of highly narrow–disperse multifunctional microspheres from visible‐light induced crosslinking of prepolymers in both a single and dual polymer system. The approach requires no stabilizers, bases, or initiators, and proceeds at ambient temperature to yield microspheres with a tunable size range (0.25–5 µm) in less than 4 h, depending largely on solvent composition, but also polymer concentration (2–10 mg mL?1), ratio, and irradiation intensity (3–20 W). Critically, the visible‐light induced dimerization reaction exploited herein enables simple functional particle syntheses via a single polymer system. Underpinned by an in‐depth kinetic analysis of the particle formation as well as a detailed small molecule study, the mechanism for particle formation is also elucidated. Importantly, inherent advantages of the system are exploited for surface functionalization of residual acrylate and hydroxyl groups (generating inherently fluorescent particles). 相似文献
179.
Ultrastructural and developmental toxicity of potato and tomato leaf extracts to beet armyworm,Spodoptera exigua (lepidoptera: noctuidae)
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Zbigniew Adamski Katarzyna Radtke Agnieszka Kopiczko Szymon Chowański Paweł Marciniak Monika Szymczak Marta Spochacz Patrizia Falabella Filomena Lelario Laura Scrano Sabino A. Bufo 《Microscopy research and technique》2016,79(10):948-958
Beet Armyworm, Spodoptera exigua is a herbivorous moth and a serious pest of many economically important plants, which are used as food sources. Because of rigorous standards of food quality, usage of synthetic insecticides in crop protection, against pests, is limited. Solanaceae plant extracts may be a relatively cheap source of efficient natural insecticides that can limit usage of synthetic substances. Their biological activity is not fully known. In particular, ultrastructural studies, using transmission electron microscopy, are not usual. In the present article we describe the effects of sublethal concentrations of tomato and potato leaf extracts against S. exigua. Acute lethal effects were not observed. Both extracts exerted similar effects within midgut and fat body cells. Midgut cells were not significantly altered while fat body cells showed prominent swelling of nuclear envelope and endoplasmic reticulum, vacuolization of mitochondria and fusion of fat droplets. These changes were much more intensive within groups exposed to potato than tomato extracts at highest concentration at least. Light microscopy was used to observe and document developmental alterations of S. exigua exposed to potato and tomato leaf extracts. Potato leaf extracts significantly decreased hatching success and caused morphological malformations of imagoes. Among them, malformations of wings were the most prominent. Interestingly, these effects were not observed within populations exposed to tomato extracts at highest concentration at least. 相似文献
180.
Large lakes have an impact on regional weather. In addition, they can be both sensitive to and influence regional climate changes. In the climate models that are used to investigate future climate changes, lakes are greatly simplified and sometimes absent. At the regional scale, this can have strong implications for the quality of the model information about the future. Through our work with climate information users in the Laurentian Great Lakes region, we have found that basic credibility of the information requires the underlying climate models simulate lake-atmosphere-land interactions. We are not aware of efforts within the scientific community to make known how individual large lakes are represented in models and how those representations translate to the quality of the data for particular regions. We share our framework for identifying how the Laurentian Great Lakes are represented in the Coupled Model Intercomparison Project (CMIP) version 5 climate models. We found that most CMIP5 models do not simulate the Great Lakes in a way that captures their impact on the regional climate, which is a credibility issue for their projections. We provide a perspective on the usability of CMIP5 for practitioners in the Great Lakes region and offer recommendations for alternative options. 相似文献