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Detailed analyses of the phospholipid compositions of cultured human endothelial cells are reported here. No significant differences
were found between the phospholipid compositions of cells from human artery, saphenous and umbilical vein. However, due to
the small sample sizes, relatively large standard deviations for some of the phospholipid classes were observed. A representative
composition of endothelial cells is: phosphatidylcholine 36.6%, choline plasmalogen 3.7%, phosphatidylethanolamine 10.2%,
ethanolamine plasmalogen 7.6%, sphingomyelin 10.8%, phosphatidylserine 7.1%, lysophosphatidylcholine 7.5%, phosphatidylinositol
3.1%, lysophosphatidylethanolamine 3.6%, phosphatidylinositol 4,5-bisphosphate 1.8%, phosphatidic acid 1.9%, phosphatidylinositol
4-phosphate 1.5%, and cardiolipin 1.9%. The cells possess high choline plasmalogen and lysophosphatidylethanolamine contents.
The other phospholipids are within the normal biological ranges expected. Phospholipids were separated by high-performance
liquid chromatography and quantified by lipid phosphorus assay. 相似文献
907.
Bret J. Chisholm Missy Berry James Bahr Jie He Jun Li Seva Balbyshev Gordon P. Bierwagen 《Journal of Coatings Technology and Research》2010,7(1):23-37
Interest in hybrid organic–inorganic (HOI) materials has grown rapidly in the last two decades. The appeal of this broad class of materials can be attributed to the unique combinations of properties that can be achieved by combining an inorganic phase with an organic phase. HOI materials can be divided into two basic categories: homogeneous systems derived from monomers or miscible organic and inorganic components, and heterogeneous, phase-separated systems with domains ranging from angstroms to micrometers in size. The structure of the inorganic component is dependent on the interaction of many variables such as pH, water content, overall solution concentration, solvent composition, temperature, and time. Due to the complexity of HOI materials, a combinatorial/high-throughput approach to the development of novel materials is highly desired. The authors have recently developed a combinatorial workflow for the synthesis and characterization of HOI coatings. Initial experimentation conducted with the workflow was focused on the development of primers for corrosion protection derived from a HOI binder system and magnesium particles. Both the homogeneous and heterogeneous HOI binders were investigated. With just one iteration of the combinatorial workflow, heterogeneous, moisture-curable HOI binders were identified, which enabled the formation of magnesium-rich primers that provided excellent corrosion protection to an aerospace aluminum alloy (Al 2024). 相似文献