The Advance Toward Distinction in Engineering Technology

This paper traces the development of engineering technology from bifurcation to the present time. They assert that ET desires to be distinguished more than it seeks to be distinct. This analysis of the successes and failures of bifurcation lead to a differentiation between engineering technology's being distinct as a discipline and becoming distinguished in its own right. They offer some conjecture about the bright future of ET as a partner with science and engineering in an atmosphere of increased technological development but intense international competition.     

Properties of permease dimer, a fusion protein containing two lactose permease molecules from Escherichia coli

An engineered fusion protein containing two tandem lactose permease molecules (permease dimer) exhibits high transport activity and is used to test the phenomenon of negative dominance. Introduction of the mutation Glu-325-->Cys into either the first or the second half of the dimer results in a 50% decrease in activity, whereas introduction of the mutation into both halves of the dimer abolishes transport. Lactose transport by permease dimer is completely inactivated by N-ethylmaleimide; however, 40-45% activity is retained after N-ethylmaleimide treatment when either the first or the second half of the dimer is replaced with a mutant devoid of cysteine residues. The observations demonstrate that both halves of the fusion protein are equally active and suggest that each half may function independently. To test the possibility that oligomerization between dimers might account for the findings, a permease dimer was constructed that contains two different deletion mutants that complement functionally when expressed as untethered molecules. Because this construct does not catalyze lactose transport to any extent whatsoever, it is unlikely that the two halves of the dimer interact or that there is an oligomeric interaction between dimers. The approach is consistent with the contention that the functional unit of lactose permease is a monomer.     

The mirror effect and mixture signal detection theory.

The mirror effect for word frequency refers to the finding that low-frequency words have higher hit rates and lower false alarm rates than high-frequency words. This result is typically interpreted in terms of conventional signal detection theory (SDT), in which case it indicates that the order of the underlying old item distributions mirrors the order of the new item distributions. However, when viewed in terms of a mixture version of SDT, the order of hits and false alarms does not necessarily imply the same order in the underlying distributions because of possible effects of mixing. A reversal in underlying distributions did not appear for fits of mixture SDT models to data from 4 experiments. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

How Meta-Analysis Increases Statistical Power.

One of the most frequently cited reasons for conducting a meta-analysis is the increase in statistical power that it affords a reviewer. This article demonstrates that fixed-effects meta-analysis increases statistical power by reducing the standard error of the weighted average effect size (T?.) and, in so doing, shrinks the confidence interval around T?.. Small confidence intervals make it more likely for reviewers to detect nonzero population effects, thereby increasing statistical power. Smaller confidence intervals also represent increased precision of the estimated population effect size. Computational examples are provided for 3 effect-size indices: d (standardized mean difference), Pearson's r, and odds ratios. Random-effects meta-analyses also may show increased statistical power and a smaller standard error of the weighted average effect size. However, the authors demonstrate that increasing the number of studies in a random-effects meta-analysis does not always increase statistical power. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

A blackboard approach to modeling expertise in corporate settlement decisions*

Abstract: The SKADE system models expertise in corporate settlement decisions using the blackboard approach. The full model has four knowledge sources: General Counsel, Attorney, Manager and Insurance Adjuster. The combined expertise from each of these is required to make the settlement decision. A control component in the model coordinates the activities of the various knowledge sources. Based on the latest data entries on the blackboard, the control selects and executes the next knowledge source. The blackboard model reproduces the experts' opportunistic reasoning processes by the interaction between the various knowledge sources. The results of analyses of a hypothetical case through a series of experiments with the SKADE system indicate that the blackboard is an appropriate model for development of multiple cooperative expert systems in the settlement decision domain. Compared to straight rule-based models, this blackboard provides more efficient problem solving. The initial success with the blackboard model suggests that further work needs to be done to see whether more complex models can be built to incorporate a broader range of determinants of settlement decisions.     

Lead differentially modifies cytokine production in vitro and in vivo

An imbalance between helper T cell type 1 (Th1) and helper T cell type 2 (Th2) activation can result in immunodysregulations leading to impaired cell-mediated immunity with an increased incidence of infectious disease or cancer and/or aberrant humoral immunity that may culminate with an autoimmune disease. Mercury, a heavy-metal toxicant, is known to induce renal autoimmunity characterized by a predominant Th2 response. Lead, another metal toxicant, causes enhanced B cell activities and impairs host resistance to several bacterial and viral infections. In addition, Pb was reported to enhance Th2 proliferation and inhibit Th1 proliferation. The differential effects of Pb on Th subset activation have been further investigated. In vitro IL-4 production by a Th2 clone was significantly increased by the addition of PbCl2, whereas IFN gamma production by a Th1 clone was decreased by the addition of PbCl2. When BALB/c mice were subcutaneously exposed to PbCl2, ex vivo Il-4 production by anti-CD3-stimulated splenic T cells was enhanced, but IFN gamma production was inhibited. Additionally, the plasma IL-4 and IgE levels of Pb-exposed mice were increased, and the plasma IFN gamma levels were significantly lowered in the absence of any additional exogenous antigen. In vitro, ex vivo, and in vivo treatment with HgCl2 produced similar findings. This study is the first report of the preferential activation of a Th2 response by Pb in vivo and suggests that PB, like Hg, may induce autoimmune responses by upsetting the balance between Th1- and Th2-like cells, which could enhance production of antibodies to self antigens.     

31P NMR identification of metabolites and pH determination in the cyanobacterium Synechocystis sp. PCC 6308

The identity of a number of phosphorus-containing metabolites present in Synechocystis sp. PCC 6308 has been confirmed by 31P NMR spectroscopy. The presence of D-ribulose 1,5-bisphosphate (RuBP); DL-glyceraldehyde 3-phosphate (GlyP); D(-)3-phosphoglyceric acid (3PGA); D-ribulose 5-phosphate (Ru5P);6-phosphogluconic acid (6PGA); phosphoenolpyruvate (PEP); inorganic phosphate (Pi); uridine diphosphoglucose (UDPG); ADP and ATP were demonstrated by the pH dependence of their 31P NMR chemical shifts in spectra of perchloric acid cell extracts. Intracellular pH of cells was determined to be 7.5-7.7.     

ATV-related central nervous system injuries in Louisiana

All terrain vehicles (ATVs) have been associated with death and serious injury since their introduction into the marketplace. Fifteen cases of ATV-related brain and spinal cord injury reported to the Louisiana Central Nervous System Registry during 1995 were evaluated for severity, etiology, and outcome. Eleven (73%) of these injuries were sustained by persons under 18 years of age. Despite US Consumer Product Safety Commission and ATV manufacturer recommendations for age restrictions, ATVs continue to be popular recreational devices for children.