Malaria chemosuppression in pregnancy. V. Placenta malarial changes among three different prophylaxis groups

The effect of malaria chemoprophylaxis during pregnancy on placenta malarial changes (PMCs) was investigated in 170 tissue sections. Women of 63 sections received daily proguanil (PROG), 61 once weekly chloroquine (CQ) and 46 the two drug combination (CQ+PROG). All were residents of a malaria hyperendemic area in Muheza District, Tanzania. Supervised prophylaxis started early in pregnancy till delivery. Parasitaemias and clinical episodes were detected early and radically treated. Overall, PMCs were mostly infrequent and light viz: fibrinous deposits (98%), fibrinoid necrosis (60%), leucocytic infiltrations (59%), macrophage containing pigment (16%), and malaria parasites (8%). The type, prevalence, and severity of the PMCs in the three prophylaxis groups were comparable. This was despite the fact that PROG and CQ+PROG were prophylactically more efficacious than CQ and despite the expectation that the prevalence and severity of the PMCs would be high in the CQ group. Prompt diagnosis and effective treatment of parasitaemias in this group contributed to the low prevalence and less severity. It is concluded that effective malaria chemoprophylaxis or prompt diagnosis and effective treatment of malaria parasitaemias have significant impact on the prevalence of PMCs. Due to various operational constraints in most developing countries, chemoprophylaxis remains the only feasible broad option for malaria control in pregnancy.     

BCL-2 overexpression attenuates cortical cell loss after traumatic brain injury in transgenic mice

The proto-oncogene, BCL-2, has been suggested to participate in cell survival during development of, and after injury to, the CNS. Transgenic (TG) mice overexpressing human Bcl-2 (n = 21) and their wild-type (WT) littermates (n = 18) were subjected to lateral controlled cortical impact brain injury. Lateral controlled cortical impact brain injury resulted in the formation of a contusion in the injured cortex at 2 days, which developed into a well-defined cavity by 7 days in both WT and TG mice. At 7 days after injury, brain-injured TG mice had a significantly reduced cortical lesion (volume = 1.99 mm3) compared with that of the injured WT mice (volume = 5.1 mm3, P < 0.01). In contrast, overexpression of BCL-2 did not affect the extent of hippocampal cell death after lateral controlled cortical impact brain injury. Analysis of motor function revealed that both brain-injured WT and TG mice exhibited significant right-sided deficits at 2 and 7 days after injury (P < 0.05 compared with the uninjured controls). Although composite neuroscores (sum of scores from forelimb and hind limb flexion, lateral pulsion, and inclined plane tests) were not different between WT and TG brain-injured mice, TG mice had a slightly but significantly reduced deficit in the inclined plane test (P < 0.05 compared to the WT mice). These data suggest that the cell death regulatory gene, BCL-2, may play a protective role in the pathophysiology of traumatic brain injury.     

Queueing analysis of buffered slotted multiple access protocols

Much of the literature on the performance evaluation of multiple access protocols has assumed a buffer capacity of one unit. This assumption is not realistic. In practice the buffer capacities used are larger than one unit in order to reduce the probability of packet rejection. This is more crucial for multiple access protocols, which allow moderate to high values of the expected throughput (URN, Random TDMA etc.).In this paper, a model appropriate for the analysis of buffered slotted multiple access schemes is proposed. The method can be applied to several multiple access protocols such as the URN protocol, the ALOHA protocol, Random TDMA etc. The cases of infinite and finite buffer capacity are examined separately but under the same basic assumptions. The analysis is based on the assumption that each user process can be modelled as an M/G/1 queueing system. The proposed method requires a small amount of computation and is characterized by a high speed, a fact that simplifies the buffer's design as well. The solution obtained is extremely accurate and exhibits excellent agreement with simulation results, which corroborate the accuracy of the model. The special case when the buffer capacity is equal to 1 is examined. In that case, the present approach also allows for computation of the packet delay distribution.     

The nuclear stethoscope: a simple device for generation of left ventricular volume curves

Initial evaluation has begun of a system for displaying left ventricular time-activity curves, relating the intraventricular content of radioactivity with the cardiac cycle as determined by the patient's electrocardiogram. Major problems include proper positioning of the detector, correction for background radioactivity outside the ventricle and calibration of the device to permit conversion of measurement of radioactivity to measurement of ventricular volumes.     

Cyclic thermo-mechanical material modelling and testing of 316 stainless steel

A programme of cyclic mechanical testing of a 316 stainless steel, at temperatures of up to 600 °C under isothermal conditions, for the identification of material constitutive constants, has been carried out using a thermo-mechanical fatigue test machine (with induction coil heating). The constitutive model adopted is a modified Chaboche unified viscoplasticity model, which can deal with both cyclic effects, such as combined isotropic and kinematic hardening, and rate-dependent effects, associated with viscoplasticity. The characterisation of 316 stainless steel is presented and compared with results from tests consisting of cyclic isothermal, as well as in-phase and out-of-phase thermo-mechanical fatigue conditions, using interpolation between the isothermal material constants to predict the material behaviour under anisothermal conditions.     

Occurrence,prevention and remediation of toxigenic fungi and mycotoxins in silage: a review

Ruminants are considered to be less sensitive towards mycotoxins than monogastric animals because rumen microbiota have mycotoxin‐detoxifying capacities. Therefore the effect of mycotoxins towards ruminants has been studied to a lesser extent compared with monogastric animals. Worldwide, a high proportion of the ruminant diet consists of silages made of forage crops (i.e. all parts of the crop above the stubble are harvested). In practice, silages are often contaminated with multiple mycotoxins. Exposure to a cocktail of mycotoxins can hamper animal production and have severe health consequences. In this article the different aspects associated with mycotoxin contamination of silage are reviewed ‘from seed to feed’. An overview is given on the occurrence of toxigenic fungal species and their concomitant mycotoxins in forage crops before and after ensiling. The mycotoxin load of visually non‐mouldy samples and mouldy hot spots within the same silo is also compared. Subsequently, this review delves into different problem‐solving strategies. A logical first step is prevention of mould growth and mycotoxin production in the field, during harvest and during ensiling. If prevention should fail, several remediation strategies are available. These are listed, mainly focusing on the possibilities of microbial degradation of mycotoxins in vivo in silage. © 2015 Society of Chemical Industry     

Iron(III) chelating resins. VI. Stability constants of iron(III)–ligand complexes on insoluble polymeric matrices

A method is presented for the determination of stability constants of iron(III)–ligand complexes on insoluble polymeric matrices based on a competition chelation reaction for iron(III) of the resin with a soluble chelator. Stability constants (K') were calculated for iron(III)–ligand complexes on DFO–Sepharose gel, HMP–Sepharose gel, AHMP–HEMA resin, and AHMP–DMAA resin. In these resins, desferrioxamine B (DFO, hexadentate ligand) or the 3-hydroxy-2methyl-4(1H)-pyridinone (HMP, bidentate ligand) was bound on insoluble polymeric matrices. The average values (log K') were: 26.6 (DFO–Sepharose); 37.9 (HMP–Sepharose); 27.2 (AHMP–HEMA); and 39.9 (AHMP–DMAA). The stability constants of the insoluble iron(III) complexes on the resins were compared with those of the corresponding soluble iron(III) complexes. The effect of immobilization on the constants was discussed, and it was found that a higher hydrophilicity and stability of a resin resulted in an increase of the stability constant, whereas steric hindrance decreased the stability constant. © 1995 John Wiley & Sons, Inc.     

Reduced serotonergic immunoreactive fibers in the forebrain of alcohol-preferring rats

Our previous study indicated that 5-hydroxytryptamine (5-HT) immunoreactive fiber densities were decreased in specific areas of the brain in alcohol-preferring rats (P) when compared with alcohol-nonpreferring rats (NP). The results of our current study show that there are quantitative and qualitative differences in 5-HT innervation in other selected regions of the forebrains of P rats. The 5-HT fiber density in the brains of young adult P and NP rats was measured by immunocytochemistry and quantitative image analysis. A routine error of two-dimensional quantitation of nerve fiber was addressed and an adjustment was made. The amount of 5-HT fibers was significantly lower in CA4 and fasciola cinereum of the dorsal hippocampus, caudate-putamen, and hypothalamus of the P as compared with NP rats (unpaired Student's t tests). In examining the fiber types, we found that, in the frontal cortical and hippocampal regions, where normally fine 5-HT fibers with small varicosities and thick 5-HT fibers with large varicosities coexist, fewer fine 5-HT fibers were seen in P rats as compared with NP rats. The fine fibers are known to be vulnerable to abusive drugs. These observations indicate that (a) there are quantitative differences in 5-HT innervation or that the 5-HT in some 5-HT fibers is reduced to a level undetectable by immunocytochemistry, and (b) the fine 5-HT fibers are specifically reduced to a greater degree in the selected brain regions of P rats when compared with that of NP rats. The involvement of the 5-HT system in the alcohol abuse is discussed.