Thermal and mechanical properties of poly(N‐isopropylacrylamide)‐based hydrogels as a function of porosity and medium change

Poly(N‐isopropylacrylamide) (PNIPAAm) has been a well‐known stimuli–responsive material and has been used in multiple novel applications. One of the key attributes to make the hydrogel more attractive is to control the response time and temperature. This work focused on comparing the physical properties, such as response time, transition temperature, heat of fusion, and mechanical strength, of macroporous and microporous PNIPAAm hydrogels, respectively. It was found that the macroporous hydrogels synthesized from a low‐temperature polymerization with addition of tetramethyl orthosilicate exhibited a faster response time and superior mechanical strength. Furthermore, to modulate the transition temperature, both the macroporous and microporous hydrogels were subjected to different qualities of media by introducing a cosolvent (methanol) or an anionic surfactant (sodium dodecyl sulfate). Interestingly, addition of a cosolvent demonstrated a more pronounced effect on the macroporous hydrogel, whereas the surfactant resulted in a more pronounced effect on the microporous hydrogel. Such results revealed that based on their porosity; there were appreciable differences when the PNIPAAm hydrogels interacted with media molecules. © 2015 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015 , 132, 42776.     

Use of a thiamine antagonist to evaluate the effects of thiamine deficiency on lake trout embryonic development

To more fully understand the secondary effects of thiamine deficiency on embryo development of lake trout Salvelinus namaycush, we used an immersion protocol to administer graded doses of the thiamine antagonist oxythiamine (OXY) (e.g., 0, 12.5, 25, 50, 100, 200, and 400 mg/L). OXY was administered to eggs of six families at water hardening (fertilization) with the intent that early mortality syndrome (EMS), an acute thiamine deficiency mortality, would only occur at the highest OXY concentration. We assessed the effect of OXY and family on the occurrence of EMS and other embryonic mortality, and alevin growth dynamics up until 2 weeks post emergence. EMS reached only 4% at the highest OXY concentration (400 mg/L) where there was a significant decline in alevin length and yolkless alevin dry weight but not the dry weight of alevins whose yolk-sac was still attached. The dry weight of the yolk-sac as a percentage of total alevin dry weight was lower than controls at low concentrations of OXY (12.5, 25 mg/L), but higher than controls at the highest concentration of OXY (400 mg/L). OXY appeared to exert its effect on growth through reduced yolk-sac utilization although the mechanism(s) involved is unknown. Reduced growth and yolk-sac utilization of alevins resulting from thiamine deficiency may contribute to mortality through decreased ability of affected alevins to secure food and avoid predation. We conclude that even for very low EMS, alevin growth effects resulting from thiamine deficiency may pose a significant impediment to lake trout reproduction.     

Reasoning in a Restricted Temporal Logic

We consider RTL, a linear time propositional temporal logic whose only modalities are the [formula] (eventually) operator and its dual [formula] (always). Although less expressive than the full temporal logic, RTL is the fragment of temporal logic that is used most often and in many verification systems. Indeed, many properties of distributed systems discussed in the literature are RTL properties. Another advantage of RTL over the full temporal logic is in the decidability procedure; while deciding satisfiability of a formula in full temporal logic is a PSPACE complete procedure, doing so for an RTL formula is in NP. We characterize the class of ω-regular languages that are definable in RTL and show simple translations between ω-regular sets and RTL formulae that define them. We explore the applications of RTL in reasoning about communication systems. Finally, we relate variants of RTL (when interpreted over a real line segments) to several fragments of Interval Modal Logic and show that the satisfiability problem for RTL when interpreted over a real line is NP-complete.     

Structure of interests in employed male and female members of US racial-ethnic minority and nonminority groups.

This study explored the structure of interests across large samples of employed U.S. racial-ethnic minority and nonminority adults drawn from over 38,000 individuals who were part of the 1994 revision of the Strong Interest Inventory (SII; L. W. Harmon, J. C. Hansen, F. H. Borgen, & A. C. Hammer, 1994; N?=?805 African Americans, 795 Asian Americans, 36,632 Caucasians, and 686 Latinos-Hispanics). Correlation matrices from the general occupational themes of the SII were subjected to 2 analyses commonly used in structural analyses of Holland's themes: randomization test of hypothesized order relations and multidimensional scaling. Analyses tested whether the data fit the circular and equidistant hexagonal structure models. All of the data fit the circular model that corresponds to Holland's calculus assumption, but the data for women and for some of the male racial-ethnic groups did not fit the more stringent equidistant hexagonal structure. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Dendritic Cells Pulsed with Cytokine-Adjuvanted Tumor Membrane Vesicles Inhibit Tumor Growth in HER2-Positive and Triple Negative Breast Cancer Models

Dendritic cells (DCs) are the most effective antigen presenting cells for the development of T cell responses. The only FDA approved DC-based immunotherapy to date is Sipuleucel-T, which utilizes a fusion protein to stimulate DCs ex vivo with GM-CSF and simultaneously deliver the antigen PAP for prostate cancer. This approach is restricted by the breadth of immunity elicited to a single antigen, and to cancers that have a defined tumor associated antigen. Other multi-antigen approaches have been restricted by poor efficacy of vaccine adjuvants. We have developed a vaccine platform that consists of autologous DCs pulsed with cytokine-adjuvanted tumor membrane vesicles (TMVs) made from tumor tissue, that encapsulate the antigenic landscape of individual tumors. Here we test the efficacy of DCs pulsed with TMVs incorporated with glycolipid-anchored immunostimulatory molecules (GPI-ISMs) in HER2-positive and triple negative breast cancer murine models. Pulsing of DCs with TMVs containing GPI-ISMs results in superior uptake of vesicles, DC activation and cytokine production. Adaptive transfer of TMV-pulsed DCs to tumor bearing mice results in the inhibition of tumor growth, reduction in lung metastasis, and an increase in immune cell infiltration into the tumors. These observations suggest that DCs pulsed with TMVs containing GPI-GM-CSF and GPI-IL-12 can be further developed to be used as a personalized immunotherapy platform for cancer treatment.     

Everyday memory failure: Age differences in appraisal and attribution.

A person perception paradigm was used to test 86 young and 84 older Ss for evidence of a double standard in appraising everyday memory failures of young and older targets. Vignettes were judged on separate Likert scales for possible attributions for the failure (ability, effort, task difficulty, chance, and 2 measures of attention), signs of mental difficulty, need for memory training, and indications of need for professional evaluation. Results confirmed a double standard used by young and old: The failures of older targets were judged as signifying greater mental difficulty and greater need for memory training than were the identical failures of young targets. Older Ss were more lenient overall than young Ss in their appraisals. Young Ss judged target persons' memory failures as signifying more mental difficulty, and they more readily recommended professional evaluation. (PsycINFO Database Record (c) 2010 APA, all rights reserved)