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51.
Anastasia V. Ponasenko Maria V. Khutornaya Anton G. Kutikhin Natalia V. Rutkovskaya Anna V. Tsepokina Natalia V. Kondyukova Arseniy E. Yuzhalin Leonid S. Barbarash 《International journal of molecular sciences》2016,17(9)
Severe bioprosthetic mitral valve calcification is a significant problem in cardiovascular surgery. Unfortunately, clinical markers did not demonstrate efficacy in prediction of severe bioprosthetic mitral valve calcification. Here, we examined whether a genomics-based approach is efficient in predicting the risk of severe bioprosthetic mitral valve calcification. A total of 124 consecutive Russian patients who underwent mitral valve replacement surgery were recruited. We investigated the associations of the inherited variation in innate immunity, lipid metabolism and calcium metabolism genes with severe bioprosthetic mitral valve calcification. Genotyping was conducted utilizing the TaqMan assay. Eight gene polymorphisms were significantly associated with severe bioprosthetic mitral valve calcification and were therefore included into stepwise logistic regression which identified male gender, the T/T genotype of the rs3775073 polymorphism within the TLR6 gene, the C/T genotype of the rs2229238 polymorphism within the IL6R gene, and the A/A genotype of the rs10455872 polymorphism within the LPA gene as independent predictors of severe bioprosthetic mitral valve calcification. The developed genomics-based model had fair predictive value with area under the receiver operating characteristic (ROC) curve of 0.73. In conclusion, our genomics-based approach is efficient for the prediction of severe bioprosthetic mitral valve calcification. 相似文献
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An assembly of hemispherical Ag nanoparticles is prepared by solid‐state dewetting of thin Ag film deposited on the sapphire substrate. The in situ nanomechanical compression testing of the particles with a flat diamond punch inside the scanning electron microscope demonstrates the deformation behavior typical for the nucleation‐controlled plasticity: high elastic deformation followed by an abrupt particles collapse. The latter is associated with the dislocations nucleation in otherwise pristine particle. The average contact pressure in the contact zone at the onset of dislocation‐controlled plasticity is about 8 GPa, and does not depend on particle size. This observation supports the hypothesis that the pseudoelasticity of much smaller Ag nanoparticles is intrinsically related to their ultrahigh strength. A stress‐induced diffusion along the particle–substrate and particle–punch interfaces is identified as a factor controlling the pseudoelastic deformation. The corresponding diffusion model allows estimating the room‐temperature self‐diffusion coefficient of Ag along the Ag–W and Ag–zirconia interfaces, which is quite close to the estimated value of the grain boundary self‐diffusion coefficient in Ag. Based on this finding, the map of pseudoelastic deformation of crystalline materials is proposed. 相似文献
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New efficient intermetallide catalysts for hydrogen storage in reversible processes of aromatics hydrogenation and naphthene dehydrogenation were studied. These catalysts provide an enhanced activity in the dehydrogenation of saturated organic molecules, with no side reactions like cracking, hydrogenolysis, ring opening, or coke formation occurring on these catalysts. The use of intermetallides provides some hydrogen storage capacity in the low-temperature region, while their catalytic activity in the dehydrogenation affords the hydrogen supply in the high-temperature range. 相似文献
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Jewgenij Botaschanjan Manfred Broy Alexander Gruler Alexander Harhurin Steffen Knapp Leonid Kof Wolfgang Paul Maria Spichkova 《Formal Aspects of Computing》2008,20(6):637-662
Formal verification of software systems is a challenge that is particularly important in the area of safety-critical automotive systems. Here, approaches like direct code verification are far too complicated, unless the verification is restricted to small textbook examples. Furthermore, the verification of application logic is of limited use in industrial context, unless the underlying operating system and the hardware are verified, too. This paper introduces a generic model stack, allowing the verification of all system layers as well as the concrete application models being used in the upper layers. The presented models and proofs close the gap between the correctness proof for the lower layers of car electronics developed at the Saarland University and the verification procedure for distributed applications developed at the Technische Universität München. 相似文献
59.
Vladislav Stroganov Jitendra Pant Georgi Stoychev Andreas Janke Dieter Jehnichen Andreas Fery Hitesh Handa Leonid Ionov 《Advanced functional materials》2018,28(11)
A novel approach for fabrication of 3D cellular structures using new thermosensitive shape‐changing polymer films with photolithographically patterned surface—4D biofabrication is reported. The surface of shape‐changing polymer films is patterned to selectively adsorb cells in specific regions. The 2D cell pattern is converted to the 3D cell structure after temperature‐induced folding of the polymer films. This approach has a great potential in the field of tissue engineering and bioscaffolds fabrication. 相似文献
60.
Alexander S. Goryashchenko Maria G. Khrenova Anna A. Bochkova Tatiana V. Ivashina Leonid M. Vinokurov Alexander P. Savitsky 《International journal of molecular sciences》2015,16(7):16642-16654
This article describes the genetically encoded caspase-3 FRET-sensor based on the terbium-binding peptide, cleavable linker with caspase-3 recognition site, and red fluorescent protein TagRFP. The engineered construction performs two induction-resonance energy transfer processes: from tryptophan of the terbium-binding peptide to Tb3+ and from sensitized Tb3+ to acceptor—the chromophore of TagRFP. Long-lived terbium-sensitized emission (microseconds), pulse excitation source, and time-resolved detection were utilized to eliminate directly excited TagRFP fluorescence and background cellular autofluorescence, which lasts a fraction of nanosecond, and thus to improve sensitivity of analyses. Furthermore the technique facilitates selective detection of fluorescence, induced by uncleaved acceptor emission. For the first time it was shown that fluorescence resonance energy transfer between sensitized terbium and TagRFP in the engineered construction can be studied via detection of microsecond TagRFP fluorescence intensities. The lifetime and distance distribution between donor and acceptor were calculated using molecular dynamics simulation. Using this data, quantum yield of terbium ions with binding peptide was estimated. 相似文献