Characterization of Inhibitory Capability on Hyperpolarization-Activated Cation Current Caused by Lutein (β,ε-Carotene-3,3′-Diol), a Dietary Xanthophyll Carotenoid

Lutein (β,ε-carotene-3,3′-diol), a xanthophyll carotenoid, is found in high concentrations in the macula of the human retina. It has been recognized to exert potential effectiveness in antioxidative and anti-inflammatory properties. However, whether and how its modifications on varying types of plasmalemmal ionic currents occur in electrically excitable cells remain incompletely answered. The current hypothesis is that lutein produces any direct adjustments on ionic currents (e.g., hyperpolarization-activated cation current, I_h [or funny current, I_f]). In the present study, GH₃-cell exposure to lutein resulted in a time-, state- and concentration-dependent reduction in I_h amplitude with an IC₅₀ value of 4.1 μM. There was a hyperpolarizing shift along the voltage axis in the steady-state activation curve of I_h in the presence of this compound, despite being void of changes in the gating charge of the curve. Under continued exposure to lutein (3 μM), further addition of oxaliplatin (10 μM) or ivabradine (3 μM) could be effective at either reversing or further decreasing lutein-induced suppression of hyperpolarization-evoked I_h, respectively. The voltage-dependent anti-clockwise hysteresis of I_h responding to long-lasting inverted isosceles-triangular ramp concentration-dependently became diminished by adding this compound. However, the addition of 10 μM lutein caused a mild but significant suppression in the amplitude of erg-mediated or A-type K⁺ currents. Under current-clamp potential recordings, the sag potential evoked by long-lasting hyperpolarizing current stimulus was reduced under cell exposure to lutein. Altogether, findings from the current observations enabled us to reflect that during cell exposure to lutein used at pharmacologically achievable concentrations, lutein-perturbed inhibition of I_h would be an ionic mechanism underlying its changes in membrane excitability.     

Effects of PCSK-9 Inhibition by Alirocumab Treatments on Biliary Cirrhotic Rats

Hyperlipidemia and oxidative stress with elevated oxidized low-density lipoprotein (ox-LDL) exacerbate hepatic inflammation and fibrosis. The plasma level of low-density lipoprotein (LDL) is controlled by proprotein convertase subtilisin/kexin 9 (PCSK9). Alirocumab is a monoclonal antibody that decreases LDL via inhibiting PCSK9 function. Apart from lipid-lowering effects, alirocumab exerts anti-inflammation, anti-angiogenesis and anti-oxidant effects. This study aims to investigate the impact of alirocumab treatment on common bile duct ligation (BDL)-induced biliary cirrhotic rats. After a 4-week treatment of alirocumab, the hemodynamic data, blood biochemistry, ox-LDL level, oxidative stress markers, severity of hepatic encephalopathy and abnormal angiogenesis of BDL rats were measured and compared to the control group. BDL rats presented cirrhotic pictures and elevated ammonia, total cholesterol, LDL and ox-LDL levels compared to the control group. Alirocumab decreased plasma levels of total cholesterol, LDL, and oxidative stress markers; however, it did not affect the hemodynamics, liver and renal biochemistry, and the plasma levels of ammonia and ox-LDL. The motor activities, portal-systemic collaterals and mesenteric vascular density were not significantly different between alirocumab-treated and control groups. In addition, it did not affect hepatic inflammation, intrahepatic angiogenesis, liver fibrosis and free cholesterol accumulation in the liver of BDL rats. In conclusion, PCSK9 inhibition by alirocumab treatment ameliorates hyperlipidemia and systemic oxidative stress in biliary cirrhotic rats. However, it does not affect the plasma level of ox-LDL, intrahepatic inflammation and fibrosis. In addition, PCSK9 inhibition has a neutral effect on abnormal angiogenesis and hepatic encephalopathy in biliary cirrhotic rats.     

阳江凹陷恩平21洼不同沉积环境烃源岩发育特征及成藏贡献

阳江凹陷为珠江口盆地近两年来获得油气勘探重大突破的新区.为明确阳江凹陷恩平21洼烃源岩的生烃潜力及成藏贡献,采用无机元素分析与有机地球化学分析相结合的研究方法,对阳江凹陷文昌组烃源岩的沉积环境、有机质来源及生烃潜力进行了系统分析,明确了已发现原油的来源并估算了不同沉积环境烃源岩的成藏贡献.结果表明:文昌组沉积时期恩平2...     

P波空间斜入射下沥青混凝土面板堆石坝响应特性及面板破坏评价

地震波入射方位和斜入射角度对于沥青混凝土面板堆石坝地震响应结果有着显著影响。本文从地震动传播机制出发,基于波场叠加原理,推导了P波以任意入射方位角和斜入射角空间斜入射下弹性半空间自由场计算公式,建立了P波空间斜入射波动输入模型。以不同应变率下的沥青混凝土单轴动态拉伸试验结果为基础,提出了考虑抗拉强度随应变率实时变化的沥青混凝土面板破坏评价方法。考虑16种不同入射方位角和斜入射角下的P波空间斜入射工况,从面板应力和加速度两方面分析了入射方位角和斜入射角对某实际沥青混凝土面板地震响应特性的影响,并进行了沥青混凝土面板的抗拉破坏评价,分析了静态评价方法与动态评价方法的差异。结果表明,所建立的P波空间斜入射波动输入模型能够准确模拟半空间自由场,波动输入数值解与解析解符合良好;与垂直输入射相比,面板顺水流向、垂直水流向加速度峰值最大分别增加135.9%和92.7%,竖向加速度峰值最大减少68.3%;空间斜输入导致面板动应力增大,主拉应力和主压应力最大分别增加3.6倍和2.7倍,忽略入射方位角和斜入射角可能会严重低估面板的地震响应。传统将静态强度提高30%作为动态强度的面板抗拉破坏判别方法过于严格,本文提出的考虑应变率效应的沥青混凝土面板破坏评价方法较为符合实际。     

多源遥感水库蓄水量估算系统

水库蓄水量研究对于合理保护和利用水资源具有重要的指导意义.针对水库蓄水量估算研究现状和不足,使用星载雷达高度计(RA)提取水库的遥测水位,利用星载多光谱成像仪(MSI)和星载合成孔径雷达(SAR)提取水库的水域面积,基于数据同化技术建立了多源遥感水库蓄水量估算模型,并开发了一款多源遥感水库蓄水量估算系统.该系统可以无接...     

新型水暖式汽车暖风装置的开发与传热特性分析

 开发了一套应用于大、中型客车上的新型水暖式汽车暖风装置.介绍暖风装置的结构、工作原理和试验方案.对暖风装置中最重要的排气热能回收装置建立数学模型和动态特性仿真模型,通过MATLAB软件对该装置传热特性进行仿真分析.对发动机在一个连续的工作过程下该装置的传热特性进行试验,结果表明,试验分析和仿真分析结果比较吻合,回收的排气热能满足汽车的供暖要求且装置在工作过程中的排气背压低于4 kPa,有很好的应用前景.     

数字水印技术综述

数字水印作为一种将特殊信息嵌入媒体数据的技术,近年来已成为国内外研究的热点并有着广泛的应用前景．通常数字水印被应用于数字图像、音频、视频以及其他媒体产品上以进行版权保护和验证多媒体数据的完整性．首先介绍了数字水印技术的特点和应用领域,并对其基本原理和评价标准进行了阐述,同时对数字水印的各种算法进行了分类研究与深入分析,并对不同算法进行了安全性与性能的横向比较,最后指出了数字水印今后的研究方向．     

Resolution enhanced light field near eye display using e‐shifting method with birefringent plate

Light field near eye displays (LFNED) can produce lightweight devices and address the accommodation–convergence conflict. However, low spatial resolution creates a poor immersive experience in LFNED. In addition, although many e‐shifting devices have been proposed to enhance resolution in projection system, yet those devices are too bulky to be applied in an LFNED to keep it lightweight. In this paper, a compact e‐shifting component is proposed to enhance image resolution in an LFNED by using a birefringent plate and twisted nematic switch cell. The proposed e‐shifting device is a flat and thin component with only 2.6 mm of thickness, which could be placed in the gap of an LFNED without increasing the thickness. The results show that the proposed components could be easily integrated in an LFNED with the result of resolution enhancement.     

网络安全的随机模型方法与评价技术

随着网络系统逐渐复杂和庞大,特别是网络攻击和破坏行为的日益普遍和多样,网络安全性面临严峻的挑战．网络安全性研究已经成为国际上重大的科学问题之一,也是影响社会经济发展和国家发展战略的重要因素．文章综述了网络安全性的随机模型与评价技术等方面的研究工作,介绍了该领域的研究现状与进展,总结了网络安全性随机模型的若干研究方法和评价技术,分析了网络的可生存性．基于这些讨论,展望了未来网络安全性研究的新方向．     

数据库的网站开发研究

本文分析了常用的网络计算模式和网站层次结构,提出了以数据库为中心的网站设计与开发思想;网站中表现出来的一切变化源于用户需求的变动,而用户需求的变动最终要抽象为数据的变化,通过网站层次结构中的中间程序处理了变化的数据,在表现层展现出来的就是用户变动了的需求,而不用去修改网站中的任何有关代码;解决了由于用户需求变动给开发及维护带来的巨大工作量问题。