A Protein-Adsorbent Hydrogel with Tunable Stiffness for Tissue Culture Demonstrates Matrix-Dependent Stiffness Responses

Although tissue culture plastic has been widely employed for cell culture, the rigidity of plastic is not physiologic. Softer hydrogels used to culture cells have not been widely adopted in part because coupling chemistries are required to covalently capture extracellular matrix (ECM) proteins and support cell adhesion. To create an in vitro system with tunable stiffnesses that readily adsorbs ECM proteins for cell culture, a novel hydrophobic hydrogel system is presented via chemically converting hydroxyl residues on the dextran backbone to methacrylate groups, thereby transforming non-protein adhesive, hydrophilic dextran to highly protein adsorbent substrates. Increasing methacrylate functionality increases the hydrophobicity in the resulting hydrogels and enhances ECM protein adsorption without additional chemical reactions. These hydrophobic hydrogels permit facile and tunable modulation of substrate stiffness independent of hydrophobicity or ECM coatings. Using this approach, it is shown that substrate stiffness and ECM adsorption work together to affect cell morphology and proliferation, but the strengths of these effects vary in different cell types. Furthermore, it is revealed that stiffness-mediated differentiation of dermal fibroblasts into myofibroblasts is modulated by the substrate ECM. The material system demonstrates remarkable simplicity and flexibility to tune ECM coatings and substrate stiffness and study their effects on cell function.     

地球物理测井技术在豫西铝土矿勘查中的应用

为了更加精准有效地勘查铝土矿,通过对以往煤田测井项目、各岩层地球物理特征的分析研究,提出了应用测井勘查铝土矿的措施。选取自然伽马等在煤田测井项目中反映较好的测井参数,通过在河南省新密、登封、偃龙、荥巩等豫西铝土矿勘探项目进行验证,将解释结果与地质编录进行比对。实践应用表明,利用自然伽马等测井参数判定铝土矿层具有一定的可行性。     

蜀南地区嘉陵江组水侵活跃气藏出水特征研究

通过研究蜀南地区嘉陵江组重点水侵活跃气藏的产水特征、影响水侵的地质基础和治水措施效果,提出了该类气藏开发不同阶段的出水特征及应采取的排水工艺措施,为确保气藏正常生产提供了技术支撑。图1表8参3     

江西某低品位钨矿石浮选试验

江西某低品位白钨矿石WO3含量为0.20%,矿物组成较复杂,金属矿物主要有白钨矿、磁黄铁矿等,非金属矿物主要有萤石、石英、透闪石、滑石、金云母、黑云母、白云母、石榴石、长石、绢云母、方解石等,含钙脉石矿物含量较高,矿石中白钨矿与脉石矿物共生关系紧密。为确定白钨矿的高效选矿工艺,进行了选矿试验研究。结果表明,矿石在磨矿细度为-0.074 mm占65%的情况下1次浮选脱硫,然后以碳酸钠为矿浆p H调整剂、水玻璃+栲胶为脉石矿物组合抑制剂、731为白钨矿浮选捕收剂,经1粗2精1扫浮选预富集钨、预富集精矿水玻璃强化调浆后1粗2精1扫常温浮选选钨、常温钨精矿90℃下水玻璃强化调浆后1粗5精1扫加温浮选选钨,最终获得WO3品位50.23%、WO3回收率为70.32%的白钨精矿,实现了白钨矿的高效回收。     

基于SDN和NFV技术的新型电力CDN

电力CDN在智能电网发展及电力信息网应用扩展过程中扮演着网络加速、流量负载、安全防护的重要角色,同时,业务需求对其灵活布置也提出了更高的要求。此外,电力CDN加速节点的扩容面临着运维复杂、资源利用率低的问题。传统的电力CDN架构亟待改革,而能够支撑电力CDN改革需求的是SDN和NFV技术。SDN技术将业务控制从网络数据和传送中抽取出来,更有利于多种业务的快速扩展以及对CDN节点的集中管控。而NFV能基于业务发展对CDN节点进行扩容和资源调度,大大减少资本支出、运营成本、空间和功耗。     

基于光照变换的Gabor小波人脸识别

光照变化易使人脸图像的灰度分布不均,造成局部对比度差别较大,会引起人脸识别正确率下降。为此在同态滤波的基础上,改变滤波函数,提出了高斯滤波的人脸识别方法,接着对滤波后的图像直方图均衡化,来增加图像的灰度动态范围,然后对人脸图像提取Gabor小波特征,最后利用最近邻法识别人脸图像。在光照变换明显的Yale B和CMU PIE数据库识别效果最好,降低了人脸图像的特征维数,缩短了特征提取时间,有效地提高了人脸识别率。     

掌握护患沟通技巧 提高护患沟通效果

目的探讨实施护患沟通的措施。方法以我院2006年1月至12月住院病人为实施对象,多层次、全方位注重护患沟通技巧的应用,实施亲情式护患沟通模式,有效提高了护理质量和患者的满意度。结果通过12个月护患沟通的实践,提高了护士的自身素质和沟通技巧,缩短了住院病人的住院天数,使住院病人的满意率提高了14.5%。结论通过护患沟通,加深了护患之间的感情,杜绝了护患纠纷的发生,满足了患者的健康需求。     

烧结主抽风机的电控系统分析

介绍了高低压电气控制系统如何保证烧结厂主抽风机正常启动、运行及监控和保护两台高压同步电机的基本原理。     

苏州丝绸博物馆的衍生品开发路径

以苏州丝绸博物馆为例,针对目前普遍存在的博物馆产品开发问题,通过实地考察,分析、总结了苏州丝绸博物馆文化衍生品开发现状。对博物馆产品开发中所遇到的困难进行了原因分析,并在依托苏州地域文化资源的前提下提出了相关解决措施,即依托丝绸文化的特质,打造衍生品的品牌效应;依托产学研合作平台,加快衍生品的市场流通速度;依托文化与科技的融合,提升衍生品的市场竞争力;依托多渠道销售,扩大文化衍生品的销售规模。苏州丝绸博物馆应当借助得天独厚的苏州丝绸文化特质,在完成博物馆的社会使命的基础上,提升博物馆的品牌内涵,实现社会效益与经济效益的双赢。     

Epoxidation of olefins with O2 and isobutyraldehyde catalyzed by cobalt (II)-containing zeolitic imidazolate framework material

Co-containing zeolitic imidazolate framework material (Co-ZIF) was prepared and its catalytic performance in the aerobic epoxidation of olefins using isobutyraldehyde as reductant under mild conditions was first studied. Co-ZIF was characterized by XRD, FT-IR and X-ray single-crystal diffraction. It showed good performance in the epoxidation of olefins, with 100% conversion, 98.5% selectivity and 638.3 turnover frequency for the epoxidation of cyclooctene. Co-ZIF could be reused for 5 times without loss of its catalytic activity and the structure of the recovered catalyst was almost unchanged compared to that of the fresh one.