Response of E. faecalis biofilms to different associations of auxiliary substances during root canal preparation: A confocal laser microscopy analysis

Objective: This in vitro study evaluated the effect of different endodontic auxiliary chemical substances over Enterococcus faecalis (Ef) biofilm through confocal laser scanning microscopy (CLSM). Methods: Forty‐five bovine incisors were infected with Ef for 21 days. Teeth were divided into five groups: group 1: 2.5% NaOCl + EDTA, group 2: 2% CHX gel + EDTA, group 3: 2% CHX liquid + EDTA, group 4: 2.5% NaOCl + 2% CHX gel + EDTA, group 5: 2.5% NaOCl + 2% CHX liquid + EDTA and a negative and a positive control group (NCG; PCG). The samples were stained with SYTO9 and propidium iodide and analyzed by CLSM. Bacterial viability was quantitatively analyzed by the proportions of dead and live bacteria in the biofilm remnants. Scores were standardized according to the total bacterial load (TBL)—1: ≤25%, 2: >25 ≤50%, 3: >50 ≤75%, 4: >75% and debris—1: absence of debris; 2: presence of debris. Statistical analysis was carried out through the Kruskal–Wallis and the Fischer exact tests (P = 0.05). Results: No statistical differences were observed to CFU, debris and bacterial viability. Conclusion: None of the tested substances could completely eliminate Ef from the root canal space. Microsc. Res. Tech. 76:658–662, 2013. © 2013 Wiley Periodicals, Inc.     

Reduction of fume and gas emissions using innovative gas metal arc welding variants

New environmental, health and safety legislation, both in the EU and in the USA, is driving the need for the study of new welding processes, and the selection of the operational procedures that will reduce fume emissions and will promote a healthier, safer and more productive work environment. Actually, there are a significant number of publications related with gas metal arc welding hazards. However, for the new gas metal arc welding hazards variants, especially cold metal transfer, there is no data available concerning fumes and gases emissions. This paper attempts to point out ways of reducing the harmful effects of gas metal arc welding processes using different filler materials, different shielding gases, different operational welding procedures and three welding processes: gas metal arc welding process and two variants, pulsed gas metal arc welding and cold metal transfer. The effect of nitrogen oxide addition to the shielding gas composition on the amount of welding fumes and gaseous emissions produced during welding is also analysed. The amount of fume and gases generated during welding was measured over a range of current intensity and arc voltages, using the standard procedures contained in EN ISO 15011-2 [1]. The data presented give a summary of the different gas metal arc welding variants and their relations to fume generation rates and gases emitted. The results obtained give indications on measures to be taken in order to reduce fume and gas emissions. In general, the minimisation of fume formation rate can be achieved by using lower energy gas metal arc welding variants, gas shielding with low CO₂ and O₂ contents and “green” wires.     

Cu-Al-Ni-SMA-Based High-Damping Composites

Recently, absorption of vibration energy by mechanical damping has attracted much attention in several fields such as vibration reduction in aircraft and automotive industries, nanoscale vibration isolations in high-precision electronics, building protection in civil engineering, etc. Typically, the most used high-damping materials are based on polymers due to their viscoelastic behavior. However, polymeric materials usually show a low elastic modulus and are not stable at relatively low temperatures (≈323 K). Therefore, alternative materials for damping applications are needed. In particular, shape memory alloys (SMAs), which intrinsically present high-damping capacity thanks to the dissipative hysteretic movement of interfaces under external stresses, are very good candidates for high-damping applications. A completely new approach was applied to produce high-damping composites with relatively high stiffness. Cu-Al-Ni shape memory alloy powders were embedded with metallic matrices of pure In, a In-10wt.%Sn alloy and In-Sn eutectic alloy. The production methodology is described. The composite microstructures and damping properties were characterized. A good particle distribution of the Cu-Al-Ni particles in the matrices was observed. The composites exhibit very high damping capacities in relatively wide temperature ranges. The methodology introduced provides versatility to control the temperature of maximum damping by adjusting the shape memory alloy composition.     

Influence of humidity,temperature, and the addition of activated carbon on the preparation of cellulose acetate membranes and their ability to remove arsenic from water

Several composite membranes have been prepared from cellulose triacetate (CTA) and activated carbon (AC) by solvent casting, varying temperature from 35 to 55°C and relative humidity (RH): 10–70%. Some conditions promoted AC particle agglomeration which is evidenced by SEM and IFME^® program. In those membranes, where homogeneity is attained, a deep characterization has been carried out by DMA, MDSC, thermoporometry, solute transport, and AFM. When AC is added in films, T_g is lowered and the fraction of pores with bigger size is augmented. Molecular weight cut off calculated by solute transport, increases from 801.15 to 1194.29 kDa using 1% AC at RH 70% and T 35°C. Water flux is of 5.23 Lm^?2 h^?1 bar^?1. Arsenic removal has been performed, achieving a 45% tested from a 500 ppb arsenic solution, where several factors such as electrical rejection, adsorption and exclusion, could contribute to the total membrane nanofiltration process. © 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014 , 131, 40134.     

Learning mobility grants and skill (mis)matching in the labour market: The case of the ‘Master and Back’ Programme

This paper looks at the geographical mobility of graduate students and their skill matching in the labour market. The paper assesses the impact of a learning mobility grant scheme funded by the European Social Fund in Sardinia (ex‐Objective 1 region in the Italian Mezzogiorno). The scheme aims to foster regional human capital and increase the employability of local graduates by covering the cost of post‐graduate studies in other regions or countries. The econometric analysis is based on a unique dataset that combines administrative data on beneficiaries with information from a dedicated survey. The results suggest that learning mobility grants can reinforce skill matching only if the problem of self‐selection of the beneficiaries is properly addressed.     

Varietal differences in terpene composition of blackcurrant (Ribes nigrum L) berries by solid phase microextraction/gas chromatography

Relative amounts of volatile terpenes in berries of 10 different blackcurrant (Ribes nigrum L) cultivars were examined by solid phase microextraction/gas chromatography (SPME/GC). The optimisation of a variety of parameters affecting SPME enabled relative standard deviations from three replicates ranging from 2 to 12% to be achieved. Differences between cultivars in the proportions rather than in the qualitative composition of volatile terpenes were found, and the proportions of some terpenes were especially variable. Furthermore, the enantiomeric ratios of the chiral terpenes were determined for the first time in blackcurrant berries by GC using a column with a stationary phase containing permethylated cyclodextrin (Chirasil‐β‐Dex). The enantiomeric compositions of the majority of the chiral terpenes varied within a reasonably narrow range. However, the levels of two monoterpene alcohols, terpinen‐4‐ol and linalool, exhibited considerable variations amongst cultivars. © 2002 Society of Chemical Industry