Histochemical study with lectins in nasal polyposis]

Changes in the membrane potential of Saccharomyces cerevisiae were monitored by the electrochromic probe 3-(4-(2-(6-(dibutylamino)-2-naphthyl)-trans- ethenyl)pyridinium)propanesulfonate (di-4-ANEPPS) that should incorporate into the plasma membrane. The probe had suitable spectral characteristics and exhibited an electrochromic shift upon a change in membrane potential but the magnitude of the response increased with time. The presence and properties of the cell wall affected the extent of cell staining. The time dependence of the fluorescent response indicated that the probe was not incorporated solely into the plasma membrane but spread gradually into the whole cell; this was confirmed by confocal microscopy. The probe is therefore suitable for assessing membrane potential changes only over time intervals up to 30 min. Longer monitoring will require either a modified staining protocol or a derivatization of the probe molecule. As found by using the dioctyl derivative di-8-ANEPPS, extending the aliphatic chains of the di-4-ANEPPS molecule does not prevent the dye from penetrating into the cell or liposome interior and, in addition, impairs staining.     

Ribose-enhanced synthesis of UTP, CTP, and GTP from parent nucleosides in cardiac myocytes

1. Characterization of allelic variants of the TPMT gene (TPMT) responsible for changes in TPMT activity, and elucidation of the mechanism by which these alleles act, are required because of the clinical importance of this polymorphism for patients receiving thiopurine drugs. 2. We defined the mutational and allelic spectrum of TPMT in a group of 191 Europeans. Using PCR-SSCP, we screened for mutation the entire coding sequence, the exon-intron boundaries, the promoter region and the 3'-flanking region of the gene. Six mutations were detected throughout the ten exons and seven TPMT alleles were characterized. Four of them, TPMT*2, *3A, *3C and *7, harbouring the known mutations, G238C, G460A, A719G or T681G, were nonfunctional and accounted for 0.5, 5.7, 0.8 and 0.3% of the allele totality, respectively. 3. Within the promoter region, six alleles corresponding to a variable number of tandem repeats (VNTR), were identified. VNTR*V4 and *V5a which harbour four or five repeats of a 17-18 bp unit, were the most frequent (55% and 34%, respectively). The other VNTR alleles, having from five to eight repeats, were rarer. 4. The TPMT phenotype was correctly predicted by genotyping for 87% of individuals. A clear negative correlation between the total number of repeats from both alleles and the TPMT activity level was observed, indicating that VNTRs contribute to interindividual variations of TPMT activity. Therefore, additional analysis of the promoter region of TPMT can improve the phenotype prediction rate by genotyping.     

Application of evolution strategies for the solution of an inverse problem in near-field optics

We introduce an inversion procedure for the characterization of a nanostructure from near-field intensity data. The method proposed is based on heuristic arguments and makes use of evolution strategies for the solution of the inverse problem as a nonlinear constrained-optimization problem. By means of some examples we illustrate the performance of our inversion method. We also discuss its possibilities and potential applications.     

Species distribution and antibiotic resistance patterns of enterococci isolated from food of animal origin in Germany

Presently, enterococci take the third place of bacterial pathogens associated with nosocomial infections, after staphylococci and Escherichia coli. Especially, the resistances of enterococci to several available antibiotics are threatening. We attempted to determine which species of enterococci could be found in food of animal origin and their significance according to their antibiotic resistances for human beings. From November 2000 to May 2002 we investigated 155 samples of food of animal origin bought in retail outlets in Germany: 27 samples of sausages, 19 of ham, 83 of minced meat, 26 of cheese. From these food samples we isolated 416 enterococcal strains. The most frequent species was Enterococcus faecalis (299 strains); furthermore, we found Enterococcus faecium (54 strains), Enterococcus durans together with Enterococcus hirae (24 strains), Enterococcus casseliflavus (22 strains), Enterococcus avium (9 strains) and Enterococcus gallinarum (8 strains). We focused on the resistance patterns of 118 selected E. faecium and E. faecalis strains to 13 antimicrobial active agents (ampicillin, amoxicillin/clavulanic acid, avilamycin, chloramphenicol, enrofloxacin, erythromycin, flavomycin, gentamicin, penicillin, quinupristin/dalfopristin, teicoplanin, tetracycline and vancomycin). From the clinical point of view, the situation of antibiotic resistance to the examined antimicrobial agents seemed to be favourable. The investigated strains were sensitive to ampicillin and amoxicillin/clavulanic acid. These antibiotics are, in combination with an aminoglycoside, for example gentamicin, agents of choice for the treatment of enterococcal infections in human medicine. Only one E. faecium strain was resistant to penicillin, while all strains were sensitive to the glycopeptide antibiotics, vancomycin and teicoplanin. Resistances found against the antibiotics, tetracycline, quinupristin/dalfopristin and erythromycin, are causes for concern.     

Guidance on individual monitoring programmes for radioisotopic techniques in molecular and cellular biology

The radioisotope techniques used in molecular and cellular biology involve external and internal irradiation risk. The personal dosemeter may be a reasonable indicator for external irradiation. However, it is necessary to control the possible internal contamination associated with the development of these techniques. The aim of this project is to analyse the most usual techniques and to establish programmes of internal monitoring for specific radionuclides (32P, 35S, 14C, 3H, 125I and 131I). To elaborate these programmes it was necessary to analyse the radioisotope techniques. Two models have been applied (NRPB and IAEA) to the more significant techniques, according to the physical and chemical nature of the radionuclides, their potential importance in occupational exposure and the possible injury to the genetic material of the cell. The results allowed the identification of the techniques with possible risk of internal contamination. It was necessary to identify groups of workers that require individual monitoring. The risk groups have been established among the professionals exposed, according to different parameters: the general characteristics of receptor, the radionuclides used (the same user can work with one, two or three radionuclides at the same time) and the results of the models applied. Also a control group was established. The study of possible intakes in these groups has been made by urinalysis and whole-body counter. The theoretical results are coherent with the experimental results. They have allowed guidance to individual monitoring to be proposed. Basically, the document shows: (1) the analysis of the radiosotopic techniques, taking into account the special containment equipment; (2) the establishment of the need of individual monitoring; and (3) the required frequency of measurements in a routine programme.     

电炉JetBOxTM技术的发展

美国PTI公司发明的专利技术JetBOxTM超音速氧枪喷射系统被证明是一种能有效提高电弧炉产量同时又能减少电炉改造成本的技术.通过在各个炼钢电炉上使用这种独特而有效的化学能输入方式从而获得了良好的效果.由于每个电炉的JetBOxTM系统都是专门设计,能满足每个炼钢车间的不同要求,因此已经被成功地应用在用料篮加料的普通电炉、Consteel电炉、使用DRI/HBI的电炉以及热装铁水的电炉上,同样都获得了良好的运行效果.自2001年以来JetBOxTM系统已经在全世界超过60台不同的电炉上安全而有效地运行着,满足了不同钢厂的要求.     

Fusion of remotely sensed data for soil moisture estimation using relevance vector and support vector machines

A data assimilation (DA) methodology that uses two state-of-the-art techniques, relevance vector machines (RVMs) and support vector machines (SVMs), is applied to retrieve surface (0–6 cm) soil moisture content (SMC) and SMC at a depth of 30 cm. RVMs and SVMs are known for their robustness, efficiency and sparseness and provide a statistically sound approach to solve inverse problems and thus to build statistical models. Here, we build a statistical model that produces acceptable estimations of SMC by using inexpensive and readily available data. The study area for this research is the Walnut Creek watershed in Ames, south-central Iowa, USA. The data were obtained from Soil Moisture Experiments 2002 (SMEX02) conducted at Ames, Iowa. The DA methodology combines remotely sensed inputs with field measurements, crop physiological characteristics, soil temperature, soil water-holding capacity and meteorological data to build a two-step model to estimate SMC using both techniques, i.e. RVMs and SVMs. First, the RVM is used to build a model that retrieves surface (0–6 cm) SMC. This information serves as a boundary condition for the second step of this model, which estimates SMC at a depth of 30 cm. An exactly similar routine is followed with an SVM for estimation of surface (0–6 cm) SMC and SMC at a depth of 30 cm. The results from the RVM and SVM models are compared and statistics show that RVMs perform better (root mean square error (RMSE)?= 0.014 m³ m^?3) when compared with SVMs (RMSE?= 0.017 m³ m^?3) with a reduced computational complexity and more suitable real-time implementation. Cross-validation techniques are used to optimize the model. Bootstrapping is used to check over/under-fitting and uncertainty in model estimates. Computations show good agreement with the actual SMC measurements with coefficients of determination (R ²) for RVM equal to 0.92 and for SVM equal to 0.88. Statistics indicate a good model generalization capability with indexes of agreement (IoAs) for RVM equal to 0.97 and for SVM equal to 0.96.     

Principles of Branching Morphology and Anatomy in Arborescent Monocotyledons and Columnar Cacti as Concept Generators for Branched Fiber‐Reinforced Composites

The branching of arborescent (tree‐like) monocotyledonous plants of the genus Dracaena or of columnar cacti differ considerably from that observed in other dicotyledonous or gymnosperm trees. The investigated ramifications exhibit distinctive morphological and anatomical features. In arborescent monocotyledons the side branches are attached to the main stem by a fiber‐reinforced tissue newly formed during secondary growth, clasping the main stem and finally resulting in a “flange‐mounted” structure. In the case of columnar cacti the most obvious feature is the pronounced constriction at the attachment point of the branches that is also mirrored in the lignified vascular tissue. One might argue that these characteristic morphological and anatomical features in regions exposed to high mechanical stresses represent structural weaknesses. However, the outer shape and the inner structures of the ramifications cause considerable stability and structural integrity of the stem‐branch connection under static and dynamic loading. Our results allow concluding that load‐adaptation in ramified plant structures is a result of a combination of optimization in outer shape and fiber arrangement within the ramifications. Numerical methods simulating the mechanical behavior based on data obtained from the studied plants support this assumption. A deeper understanding of the outer shape of the connection between shoot and branch as well as of the arrangement of the lignified vascular tissues in the branching region, may contribute toward alternative concepts for branched technical light‐weight‐structures. In particular for braided fiber‐reinforced composites this biomimetic approach might help to keep the demand on the available design space as small as possible.