Erythrocyte-Derived Nanoparticles with Folate Functionalization for Near Infrared Pulsed Laser-Mediated Photo-Chemotherapy of Tumors

Despite its common side effects and varying degrees of therapeutic success, chemotherapy remains the gold standard method for treatment of cancer. Towards developing a new therapeutic approach, we have engineered nanoparticles derived from erythrocytes that contain indocyanine green as a photo-activated agent that enables near infrared photothermal heating, and doxorubicin hydrochloride (DOX) as a chemotherapeutic drug. We hypothesize that milliseconds pulsed laser irradiation results in rapid heating and photo-triggered release of DOX, providing a dual photo-chemo therapeutic mechanism for tumor destruction. Additionally, the surface of the nanoparticles is functionalized with folate to target the folate receptor-α on tumor cells to further enhance the therapeutic efficacy. Using non-contract infrared radiometry and absorption spectroscopy, we have characterized the photothermal response and photostability of the nanoparticles to pulsed laser irradiation. Our in vitro studies show that these nanoparticles can mediate photo-chemo killing of SKOV3 ovarian cancer cells when activated by pulsed laser irradiation. We further demonstrate that this dual photo-chemo therapeutic approach is effective in reducing the volume of tumor implants in mice and elicits an apoptotic response. This treatment modality presents a promising approach in destruction of small tumor nodules.     

Aerodynamic response of an airfoil section undergoing pitch motion and trailing edge flap deflection: a comparison of simulation methods

The study presents and compares aerodynamic simulations for an airfoil section with an adaptive trailing edge flap, which deflects following a smooth deformation shape. The simulations are carried out with three substantially different methods: a Reynolds‐averaged Navier–Stokes solver, a viscous–inviscid interaction method and an engineering dynamic stall model suitable for implementation in aeroelastic codes based on blade element momentum theory. The aerodynamic integral forces and pitching moment coefficients are first determined in steady conditions, at angles of attack spanning from attached flow to separated conditions and accounting for the effects of flap deflection; the steady results from the Navier–Stokes solver and the viscous–inviscid interaction method are used as input data for the simpler dynamic stall model. The paper characterizes then the dynamics of the unsteady forces and moments generated by the airfoil undergoing harmonic pitching motions and harmonic flap deflections. The unsteady aerodynamic coefficients exhibit significant variations over the corresponding steady‐state values. The dynamic characteristics of the unsteady response are predicted with an excellent agreement among the investigated methods at attached flow conditions, both for airfoil pitching and flap deflection. At high angles of attack, where flow separation is encountered, the methods still depict similar overall dynamics, but larger discrepancies are reported, especially for the simpler engineering method. Copyright © 2014 John Wiley & Sons, Ltd.     

Polystyrene/kaolinite nanocomposite synthesis and characterization via in situ emulsion polymerization

Polymer Bulletin - The polymer nanocomposites polystyrene (PS)/kaolinite (Kao) were synthesized by in situ emulsion polymerization. First Kao was modified using dimethylsulfoxide (DMSO) by a...     

Misconceptions in the application of the Sieverts technique

The Sieverts technique is commonly used for determining gas uptake, particularly for hydrogen storage material characterisation. Despite a considerable literature on correct technique, best practice and sources of potential errors, there is still confusion concerning application of this technique. We address three aspects of the Sieverts technique, (thermal transpiration, volume calibration and the effect of valve volume) in the context of a recent publication.     

Global assessment of polybrominated diphenyl ethers in farmed and wild salmon

We have shown recently that levels of persistent, bioaccumulative contaminants (polychlorinated biphenyls, dioxins, and several chlorinated pesticides) are significantly higher in farmed than in wild salmon and that European farm-raised salmon have significantly greater toxic contaminant loads than those raised in North and South America. In this paper, we extend these results to polybrominated diphenyl ethers (PBDEs) and show that farm-raised salmon have higher levels of these compounds than wild salmon. We also show that farm-raised salmon from Europe have higher PBDE levels than those raised in North America and that both European and North American farm-raised salmon have higher PBDE levels than those farm-raised in Chile. Among the species of wild salmon, chinook had significantly elevated PBDE levels relative to the other wild species. These elevated PBDE levels may be related to chinook's feeding behavior and trophic level. Among all of the wild species we studied, chinook tend to feed higher in the food web throughout their adult life and grow to be larger individuals.     

A 3D Cell-Free Bone Model Shows Collagen Mineralization is Driven and Controlled by the Matrix

Osteons, the main organizational components of human compact bone, are cylindrical structures composed of layers of mineralized collagen fibrils, called lamellae. These lamellae have different orientations, different degrees of organization, and different degrees of mineralization where the intrafibrillar and extrafibrillar minerals are intergrown into one continuous network of oriented crystals. While cellular activity is clearly the source of the organic matrix, recent in vitro studies call into question whether the cells are also involved in matrix mineralization and suggest that this process could be simply driven by the interactions of the mineral with extracellular matrix. Through the remineralization of demineralized bone matrix, the complete multiscale reconstruction of the 3D structure and composition of the osteon without cellular involvement are demonstrated. Then, this cell-free in vitro system is explored as a realistic, functional model for the in situ investigation of matrix-controlled mineralization processes. Combined Raman and electron microscopy indicate that glycosaminoglycans (GAGs) play a more prominent role than generally assumed in the matrix–mineral interactions. The experiments also show that the organization of the collagen is in part a result of its interaction with the developing mineral.