Measuring cellular structure at submicrometer scale with lightscattering spectroscopy

We present a novel instrument for imaging the angular distributions of light backscattered by biological cells and tissues. The intensities in different regions of the image are due to scatterers of different sizes. We exploit this to study scattering from particles smaller than the wavelength of light used, even when they are mixed with larger particles. We show that the scattering from subcellular structure in both normal and cancerous human cells is best fitted to inverse power-law distributions for the sizes of the scattering objects, and propose that the distribution of scattering objects may be different in normal versus cancerous cells     

Binding of organic anions by synthetic supramolecular metallopores with internal Mg2+-aspartate complexes

We report that cation-selective transmembrane pores formed by synthetic p-octiphenyl beta barrels with internal aspartate residues can be transformed into anion-permeable metallopores with internal Mg(2+)-aspartate complexes. These metallopores are shown to be useful for fluorimetric sensing of a broad variety of organic anions of biological relevance such as phytate, heparin, thiamine phosphates, and adenosine triphosphate. The negligible flippase activity measurable for Mg(2+)-free pores indicates that transmembrane p-octiphenyl beta barrels do not disturb the lipid bilayer suprastructure, in other words, they form barrel-stave rather than toroidal pores.     

Kinematic analysis of a flexible six-DOF parallel mechanism.

In this paper, a new type of six-degrees of freedom (DOF) flexible parallel mechanism (FPM) is presented. This type of parallel mechanism possesses several favorable properties: (1) its number of DOFs is independent of the number of serial chains which make up the mechanism; (2) it has no kinematical singularities; (3) it is designed to move on rails, and therefore its workspace is much larger than that of a conventional parallel manipulator; and (4) without changing the number of DOFs and the kinematics of the mechanisms, the number of the serial chains can be reconfigured according to the needs of the tasks. These properties make the mechanism very preferable in practice, especially for such tasks as joining huge ship blocks, in which the manipulated objects vary dramatically both in weights and dimensions. Furthermore, the mechanism can be used as either a fully actuated system or an underactuated system. In the fully actuated case, the mechanism has six DOF motion capabilities and manipulation capabilities. However, in the underactuated case, the mechanism still has six DOF motion capabilities, but it has only five DOF manipulation capabilities. In this paper, both the inverse and forward kinematics are studied and expressed in a closed form. The workspace and singularity analysis of the mechanism are also presented. An example is presented to illustrate how to calculate the kinematics of the mechanism in both fully-actuated and underactuated cases. Finally, an application of such a mechanism to manufacturing industry is introduced.     

Secure electronic markets for private information

Technological advances in the collection, storage, and analysis of data have increased the ease with which businesses can make profitable use of information about individuals. Some of this information is private, and individuals are simultaneously becoming more aware of the value of the information and how the loss of control over this information impacts their personal privacy. As a partial solution to these concerns, this paper presents a mechanism that serves two purposes. The first enables the use of private, numerical data in the answering of queries while simultaneously providing a security feature that protects the data owners from a loss of privacy that could result from an unauthorized access. The second develops a compensation model for the use of the data that allows individuals to dynamically redefine their security requirements. The compensation model is built on the information-security mechanism to create the foundation of a market for private information. This paper illustrates how compensation models like the one presented here could be used in a self-regulating market for private information. Additionally, the compensation component of an intermediated market for private information is developed and extensively analyzed. Finally, this paper provides insights and draws several important conclusions on markets for private information.     

Enhanced layered segment trees: a pragmatic data structure for real-time processing of geometric objects

The data structure that is probably most used in the pattern recognition and image processing of geometric objects is the segment tree and its optimized variant, the “layered segment tree”. In all the versions currently known, except the work of Vaishnavi and Wood described later, these data structures do not operate in real time. Even in the latter scheme, although the structure can be implemented in real time and in an on-line fashion, the operation of insertion involves the sorting of the representations of the line segments in the tree. In essence, for all the reported algorithms, there is no known strategy to insert the segments one by one, other than the trivial strategy of processing them all together as in a batched-mode. In this paper, we present a strategy in which all the operations done on the tree can be done efficiently. Indeed, by improving the bottleneck, we prove that an arbitrary horizontal segment can be inserted into this data structure without invoking an expensive sorting process. We show that while this is accomplished by maintaining the same space and query complexity of the best-known algorithm, the version presented here is applicable to on-line real-time processing of line segments. The paper thus has applications in all areas of pattern recognition and image processing involving geometric objects.     

A new class of intelligent knowledge-based systems with an optimisation-based inference engine

In this paper we describe a new class of intelligent knowledge-based system (IKBS) which can be used principally for managerial decision making applications. This class of applications often requires a framework for knowledge acquisition which allows the system to use the knowledge of several experts. In addition, since in most business decision making the objective is maximise profits, there is a need for an inference engine which allows optimisation to be carried out. The new class of IKBS which is described in this paper has both these properties, i.e., the ability to use the knowledge of multiple experts in a convenient way and an inference engine which by performing optimisations is able to pick out the profit maximising decisions. As an illustration of these concepts, a system for allocation decision making is described. The system ‘Retail-opt’ allows the user to solve problems like allocation of space in retail outlets, allocation of space in mail order catalogues, pricing policy decisions for discounted airline tickets, etc. In the paper, the basic concepts behind ‘Retail-opt’ are described and an application of ‘Retail-opt’ to the problem of retail space allocation in a Scandinavian Department Store is given. A number of other systems which use these concepts for more complicated competitive decision making situations are also described.     

Kinetics of oxygen dissolution in molten lead

An electromotive force cell employing the ZrO₂-CaO solid electrolyte has been set up to investigate the kinetics of dissolution of oxygen in molten lead under various oxidizing conditions. The cell may be written as The cell has been so designed that the electrode chambers are completely isolated from each other. The oxygen in lead is first reduced to a very low value by bubbling hydrogen. subsequently, oxygen dissolution is achieved through gas diffusion across the free surface or through bubble metal interaction. During all these reactions the electromotive force of the cell changes continuously and is automatically recorded. The diffusion coefficient of oxygen in lead, as calculated from the experimental data, is 1.29×10⁻⁵ sq cm per sec and the liquid phase mass transfer coefficient is estimated to be 0.035 cm per sec at 750°C.     

The magnetic field problem: A graph-theoretic model

A numerical model for two-dimensional magnetic field problems called the graph-theoretic field model is presented. The theoretical framework of the model places the finite difference and Finite element method in a unifying perspective and brings the solution of magnetic field problems into the well-proven realm of computer-aided design of discrete physical systems. The field distribution in a transformer core is obtained to illustrate the modeling procedure.     

Growth characteristics of two interacting cracks

This paper deals with the growth characteristics of two interacting cracks in a mixed mode condition. The problem considered is that of two cracks of different lengths, arbitrarily placed in a plane. A uniform load is applied in the plane at a large distance away from the cracks. The objectives of this study are threefold: (1) Solve the mixed boundary value problem described in the foregoing; (2) Predict the most vulnerable crack tip where the instability would first occur as the load is increased; (3) Determine the directions in which the cracks may grow. The numerical results given are only for the specific case of one crack lying along a radial line which emanates from the tip of the other crack at an arbitrary angle.