Natalie Munding;Magdalena Fladung;Yi Chen;Marc Hippler;Anthony D. Ho;Martin Wegener;Martin Bastmeyer;Motomu Tanaka; 《Advanced functional materials》2024,34(20):2470111

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Bukola M. Adenuga  Magdalena Montowska 《Comprehensive Reviews in Food Science and Food Safety》2023,22(3):2112-2160

Despite the numerous studies on food safety and authenticity, especially for meat and meat products, not enough studies have been conducted focusing exclusively on game species and other unusual meat animals. As a result of the European horse scandal, the horse is currently the target of many meat authenticity studies. With this review, we aim to present various DNA-based methods that have been used by researchers to identify, detect, and quantify game, uncommon meat animals, and wildlife species. Polymerase chain reaction (PCR) is considered the standard method for DNA analysis in meat authenticity testing. However, in this paper, we present several other methods that may or may not involve the PCR technique. For this purpose, we systematically reviewed 131 articles selected according to various criteria such as target animal species, method of analysis, year of publication, and so forth. The result of our study shows the most studied game and uncommon meat species, PCR- and non-PCR-based methods for game meat analysis, and DNA-based methods in wildlife conservation. With this study, researchers can find detailed information about frequent game species used as adulterants for regular meat products and the DNA-based techniques to identify them.  相似文献   

    

Prashamsa Koirala  Sung-Po R. Chen  Jennifer C. Boer  Zeinab G. Khalil  Cyril Deceneux  Georgia Goodchild  Lantian Lu  Mohammad Omer Faruck  Ahmed O. Shalash  Sahra Bashiri  Robert J. Capon  Waleed M. Hussein  Michael J. Monteiro  Magdalena Plebanski  Istvan Toth  Mariusz Skwarczynski 《Advanced functional materials》2023,33(12):2209304

Antigens incorporated in subunit vaccines are typically poorly immunogenic, so a strong immunostimulant (adjuvant) and/or delivery system is required to boost immunogenicity. In this work, the various functional polymer nanostructures, that is, rods, worms, spheres, and tadpoles are used to develop potent peptide antigen delivery systems. The antigen PADRE-J8 (PJ8), derived from Group A Streptococcus (GAS) M-protein, is either physically mixed or chemically conjugated to polymeric nanoparticles of different shapes. The physical mixture of polymeric nanoparticles and antigen is more effective in inducing antibody production than their chemical conjugates. Moreover, rod-shaped polymeric nanoparticles in physical mixture with PJ8 elicited higher and more opsonic antibody titers than powerful complete Freund's adjuvant (CFA)-adjuvanted antigen. Herein, for the first time it is demonstrated that a) the block copolymer, in nanoparticle form, can act as an immune adjuvant, b) nanoparticle shape plays a crucial role in their immunogenicity, and c) antigen conjugation is not required, nor is antigen encapsulation or absorption.  相似文献   

    

Marta Matuszewska  Magdalena Cielik  Anna Wilkaniec  Marcin Strawski  Grzegorz A. Czapski 《International journal of molecular sciences》2023,24(1)

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Magdalena Laskowska  Pawe&#x; Kowalczyk  Agnieszka Karczmarska  Karol Kramkowski  Karol Wrzosek  &#x;ukasz Laskowski 《International journal of molecular sciences》2023,24(1)

This article is devoted to a novel class of antimicrobial agents: nanocomposites composed of spherical silica and silver ions located at the silica’s surface with the assumed distribution. Such materials are in high demand due to the increasing threat from bacterial strains that are becoming resistant to currently known antibiotics. In particular, we focus on materials that make it possible to limit the growth of bacterial colonies on a variety of tactile surfaces. In this paper, we present a method for preparing a silica-based nanocomposite containing silver ions and the analysis of their antimicrobial properties. Our research revealed that the presence of tested nanocomposite induces very high oxidative stress in the bacteria cell, damaging and modifying bacterial DNA, creating oxidized guanines, cytosines, or adenines, which causes its very rapid destruction, leading to cell death.  相似文献   

    

Leszek Roszkowski  Boena Jaszczyk  Magdalena Pleba&#x;czyk  Marzena Ciechomska 《International journal of molecular sciences》2023,24(1)

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Anna Syku&#x;a  Adriana Nowak  Eugenio Garribba  Aliaksandr Dzeikala  Magdalena Rowi&#x;ska-yrek  Justyna Czerwi&#x;ska  Waldemar Maniukiewicz  Elbieta &#x;odyga-Chruci&#x;ska 《International journal of molecular sciences》2023,24(1)

The three Schiff base ligands, derivatives of hesperetin, HHSB (N-[2,3-dihydro-5,7-dihydroxy-2-(3-hydroxy-4-methoxyphenyl)chromen-4-ylidene]isonicotinohydrazide), HIN (N-[2,3-dihydro-5,7-dihydroxy-2-(3-hydroxy-4-methoxyphenyl)chromen-4-ylidene]benzhydrazide) and HTSC (N-[2,3-dihydro-5,7-dihydroxy-2-(3-hydroxy-4-methoxyphenyl)chromen-4-ylidene]thiosemicarbazide) and their copper complexes, CuHHSB, CuHIN, and CuHTSC were designed, synthesized and analyzed in terms of their spectral characterization and the genotoxic activity. Their structures were established using several methods: elemental analysis, FT-IR, UV-Vis, EPR, and ESI-MS. Spectral data showed that in the acetate complexes the tested Schiff bases act as neutral tridentate ligand coordinating to the copper ion through two oxygen (or oxygen and sulphur) donor atoms and a nitrogen donor atom. EPR measurements indicate that in solution the complexes keep their structures with the ligands remaining bound to copper(II) in a tridentate fashion with (O^–, N, O_ket) or (O^–, N, S) donor set. The genotoxic activity of the compounds was tested against model tumour (HeLa and Caco-2) and normal (LLC-PK1) cell lines. In HeLa cells the genotoxicity for all tested compounds was noticed, for HHSB and CuHHSB was the highest, for HTSC and CuHTSC–the lowest. Generally, Cu complexes displayed lower genotoxicity to HeLa cells than ligands. In the case of Caco-2 cell line HHSB and HTSC induced the strongest breaks to DNA. On the other side, CuHHSB and CuHTSC induced the highest DNA damage against LLC-PK1.  相似文献   

    

Magdalena Wiśniewska  Dariusz R. Mańkowski  Anna Fraś 《Journal of the science of food and agriculture》2024,104(1):286-294

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Francisco Suárez;Tomás Oportus;Magdalena Mendoza;Ignacio Aguirre;Víctor Godoy;José F. Muñoz; 《Wiley Interdisciplinary Reviews: Water》2024,11(1):e1638

Evapotranspiration has been poorly characterized in the Altiplano, and the legal dispute over the status and use of the waters of the Silala River provided an opportunity for improving knowledge of this hydrological process, in a remote area with a lack of hydrometeorological data. Actual evapotranspiration (ETa) measurements using eddy covariance (EC) were performed in the riparian wetland (EC1) and over the alluvial deposits (EC2) of the Silala River basin, and in the Putana River wetland (EC3), with similar characteristics to the Silala River headwaters. The portable chamber method was used to characterize soil and plant evaporation near EC2. Satellite-derived ETa estimates were evaluated with the in-situ data, and used to investigate spatiotemporal dynamics of the wetland vegetation cover. At EC1 and EC3, annual ETa values were 703 and 841 mm, respectively; and satellite-derived ETa showed differences of 5% and −35% from these values. ETa exhibited strong seasonal variations at EC1 and EC3, and was sensitive to precipitation at EC2. ETa did not follow the temporal evolution of the reference evapotranspiration at EC2, suggesting that lateral subsurface flow supports ETa in dry periods. Portable chamber measurements revealed that bare soil evaporation is the main component of ETa in the alluvial deposits. ETa data collected within the basin, as described in other papers of this special issue, allowed validation of a hydrological model used to estimate the basin's groundwater recharge, and estimation of the surface flow increase due to river channelization, which were important scientific evidence provided in the legal dispute.  相似文献   

    

Gonzalo Yáñez-Morroni;Francisco Suárez;José F. Muñoz;Magdalena Sofía Lagos; 《Wiley Interdisciplinary Reviews: Water》2024,11(1):e1690

The Silala is a small river, originating in the Andean Altiplano, which flows from Bolivia into Chile. Prior to a legal dispute between Chile and Bolivia over the status and use of the waters of the Silala, few hydrological studies had been performed in the basin. Further insights were required to better understand the surface-water and groundwater discharges from Bolivia to Chile, and the effects of historical channelization of the Bolivian wetlands on these flows. A semi-distributed hydrological model was therefore developed to estimate the discharges from the basin and provide recharge inputs to a groundwater model used to investigate the effects of channelization. Long-term temperature and precipitation data were available for 1969–1992, while more detailed data were available for 2018–2019. 1969–1992 was selected as a suitable length of record for long-term groundwater recharge estimation, and the recent data were reserved for model validation, reported in a companion paper. Prior model parameter ranges were identified based on field observations and scientific literature, and sampling of both input and parameter uncertainty allowed determination of representative, lower and upper groundwater recharge scenarios. Results show strong inter-annual and seasonal variability, the largest groundwater recharge being observed during the Austral summer. A representative groundwater recharge rate of 39.5 mm/year was obtained for the basin to the international border, with feasible lower and upper bounds of 34.9 and 50.2 mm/year, respectively. This lies within the range of 21–51 mm/year estimated by Bolivia for 1969–2017, albeit higher than their best estimate (24 mm/year).  相似文献