猪瘟病毒兔化弱毒(HCLV)疫苗株基因组全长cDNA的克隆与序列分析

根据猪瘟病毒基因组的序列设计合成了覆盖HCLV基因组全部序列的5对引物，通过RT-PCR从感染家兔脾脏中扩增获得了包含HCLV(hog cho1era lapinized virus,HCLV)基因组全序列的5个cDNA片段，并将它们分别克隆至pGEM-T vector中。然后将这5个cDNA片段按它们在HCLV基因组上的位置从5’端至3’端的顺序依次通过酶切连接，一并克隆到pPo1y2载体中得到HCLV基因组全长cDNA的质粒pPOHCLV。序列分析表明，HCLV基因组长度共12310bp，有一个大的ORF，编码-3898个氨基酸的多聚蛋白。其5’—NCR和3’—NCR长度分别是374nt和239nt。与非兔化毒株相比较，HCLV基因组在12133nt处有12个碱基的插入CTTTTTTCTTTT，该序列可能是病毒在适应兔体增殖过程中形成的。基于基因组全序列及其所推导的氨基酸序列的同源性分析表明，毒株的亲缘关系的远近与它们的毒力之间并没有相关性。     

SPA用于丝素膜的生物改性研究

用生长因子RGD半抗原（GLY-ARG-GLY-ASP-SER-PRO-LYS)连接到卵清蛋白Ovalbumin(OVA)载体上诱发出了抗RGD抗体IgG，并用丝素溶液包埋SPA(Staphylo-coccal protein A，简称A蛋白或SPA)制成不溶性SPA丝素膜为材料，然后用诱发出的RGD抗体IgG结合到不溶性SPA丝素膜的表面，制成IgG-SPA丝素膜，再在其上结合粘附生长因子RGD，制成RGD-IgG-SPA丝素膜。利用这一丝素膜培养血管内皮细胞(Vas-cular Endothelial Cell，简称EC细胞)，用四甲基偶氮些盐比色方法(MTT法)检测细胞的生长增殖情况。结果表明，RGD-IgG-SPA丝素膜能有效促进EC细胞的生长。对不溶性SPA丝素膜和IgG以及IgG和RGD之间的生物结合力测定，表明其结合力远大于离解力。同时在细胞培养液中没有检测到丝素膜中SPA的渗漏。RGD-IgG-SPA丝素膜为其作具有的这些优良性质为血管支架打下了基础。     

PP及芳纶/PP结晶性能的分析研究

本文通过DSC对PP及芳纶／PP复合体系结晶性能的分析研究，得到了PP长丝热压成膜结晶性能的变化，芳纶对PP结晶性能的影响以及不同冷却方式对芳纶／PP结晶性的影响等规律。     

人造金刚石合成块物理除杂提纯方法研究

本文简要分析了国内人造金刚石提纯技术现状，论述了人造金刚石物料的基本力学性质及解离特性，研究探讨了一种符合环保要求、合理利用物理选矿原理对人造金刚石进行除杂提纯的方法。利用几种新设备分阶段选择性破碎细化，通过筛分分级并借助重选和磁分离等方法，分别除去绝大部分石墨、合金碎屑、叶蜡石等杂质，得到的人造金刚石只需进行低浓度酸碱处理。本法明显降低酸碱用量，减少环境污染，降低生产成本；劳动生产率高，效益好。     

Load testing, structural reliability and test evaluation

A brief review of the history and types of load testing is followed by an examination of the effects of load test and other information on structural reliability. Two aspects are studied. (1) Multi-mode proof testing is posed as a system relability problem with one or more inequality conditions on structural performance. After-test reliability can then be found using standard system reliability methods from the reliability of the system augmented in “series” with the test mode, divided by the reliability in the test mode alone. (2) Test evaluation procedures for several applications are analysed using simulation of destructive sample tests, including strength uncertainty discoverable by testing and other non-test uncertainties in strength and loading. Evaluation rules are found to have a significant effect on the achieved reliability of test-based design.     

基于解析冗余式的风机传动系统故障诊断

为了以系统有序的方法对风机的具体元件参数进行故障诊断,给出一种基于键合图模型的故障诊断方法。键合图模型是一种跨能域的元件级模型,因此能够定位到具体的故障元件。同时键合图模型能够清晰地表明各元件之间的关系,因此适用于推导解析冗余式。通过解析冗余式可以对系统进行故障检测和隔离。为了能够系统地得到尽量多的解析冗余式,提高故障的可隔离性,该方法首先建立风机的键合图模型,然后由键合图模型导出时间因果图,由时间因果图导出变量关系图,最后由变量关系图消除键合图中结点方程的未知变量得到解析冗余式。实验结果验证了采用该方法推导的解析冗余式能够用于风机参数故障的诊断。     

考虑吸附相视孔隙度的页岩气藏物质平衡方程

页岩气藏储量是确定气藏开发规模和开发设计的必要参数,对于合理有效开发页岩气藏具有重要意义。在前人研究的基础上,建立了综合考虑吸附相视孔隙度、基质和裂缝孔隙体积随压力变化以及吸附相密度等因素的物质平衡方程。通过实例计算可知:考虑吸附相视孔隙度后,计算得到的基质中自由气储量大幅度减少,吸附气储量大幅度增加,总储量增加;而且吸附相密度、基质孔隙度和裂缝压缩系数对于储量的计算结果也有较大影响。     

Surfactant-assisted synthesis of reduced graphene oxide/polyaniline composites by gamma irradiation for supercapacitors

A series of graphene materials are prepared by intercalation of graphene oxide (GO) with different surfactants, cetyltrimethylammonium bromide (CTAB), n-octyltrimethylammonium bromide, tetramethylammonium bromide, and sodium dodecylbenzene sulfonate, subsequently by γ-ray induced reduction in N-methyl-2-pyrrolidone (NMP) at room temperature. GO can be reduced by the electrons generated from the radiolysis of NMP under γ-ray irradiation, and reduced GO is simultaneously functionalized by the radiolytic product of NMP. Cationic surfactant CTAB with longer alkyl chains can effectively promote the reduction process of GO by preventing the aggregation of graphene sheets, which has been testified by X-ray photoelectron spectroscopy, X-ray diffraction, thermogravimetric analysis, Raman spectroscopy, and Fourier transform infrared spectroscopy analyses. Furthermore, when the as-prepared graphene/polyaniline composites are used for supercapacitor electrode materials, there is a highest specific capacitance of 484 F g^?1 at a current density of 0.1 A g^?1 for the graphene produced in the presence of cationic surfactant CTAB.     

南京四桥索鞍吊装系统刚柔耦合动力学仿真

以南京长江第四大桥的散索鞍（北锚）吊装系统为原型,综合应用UG、Ansys和ADAMS对索鞍吊装过程进行刚柔耦合动力学仿真分析,并将分析的数据成功地应用到索鞍吊装系统的设计优化中,为随后索鞍顺利吊装就位打下了坚实的理论基础。     

碳氢燃料自燃化学特性的实验研究

本文介绍了标准参考燃料及其混合物化自然化学特性的实验研究结果。