Quality assurance concepts for adhesive bonding of composite aircraft structures – characterisation of adherent surfaces by extended NDT

In order to ensure the performance of adhesively joined load-critical composite structures, suitable technologies are needed to steadily monitor adherent surfaces prior to bonding and to detect adhesion properties of bonded components. A novel class of non-destructive testing (NDT) techniques, classified as extended non-destructive testing (ENDT), is required to ascertain selected physicochemical properties which are important for the performance of adhesive bonds in place of detecting material defects like conventional NDT methods do. The European FP7 project, ‘ENCOMB – Extended non-destructive testing of composite bonds’ aims in the identification, development, adaptation and validation of ENDT methods for characterisation of adherent surfaces and adhesive bond quality. Here, recent NDT techniques such as optically stimulated electron emission (OSEE) and aerosol wetting test (AWT) as well as laser-induced breakdown spectroscopy (LIBS) were advanced and applied in field, and without contacting carbon fibre-reinforced polymer (CFRP) surfaces for detecting different contamination layers such as release agent, moisture or hydraulic oil as well as thermal degradation of CFRP adherent surfaces before performing an adhesive bonding process. Sensitivity and accuracy of these techniques allow distinguishing surface states which are suitable for bonding of CFRP adherents from surface states which are unfavourable for bonding. ENDT using OSEE, AWT and LIBS facilitated the detection of layers of release agent as thin as one nanometre and thin layers resulting from hydraulic oil. OSEE investigations of adherent surfaces before adhesive bonding allowed the indication of all surface states of potential CFRP adherents, which according to previous studies, were related to application scenarios reducing the joint strength of resulting adhesive joints by 20–70%.     

Reliable identification of closely related Issatchenkia and Pichia species using artificial neural network analysis of Fourier‐transform infrared spectra

A reliable identification system for closely related species of the genera Issatchenkia and Pichia was established, using artificial neural network‐based Fourier‐transform infrared (FTIR) spectroscopy; 16 common Pichia species and all five known Issatchenkia species were included. A total of 238 strains isolated from a large variety of habitats were used as reference strains to generate an artificial neural network (ANN) identification system. This system consists of 10 single subnets connected to an ANN with four consecutive levels. An internal validation of the system, using unknown spectra of each reference strain, yielded an identification rate of 99.2%. To evaluate the performance of the ANN in routine diagnostics, 1608 spectra of 179 strains unknown to the ANN were used as a test dataset in an external validation. An overall identification rate of 98.6%, including a success rate of 100% for two common species, P. anomala and P. membranifaciens, demonstrates considerable potential of this FTIR‐based artificial neural network for the identification of closely related yeast species. Copyright © 2008 John Wiley & Sons, Ltd.     

Adhesion of Neurons and Glial Cells with Nanocolumnar TiN Films for Brain-Machine Interfaces

Coupling of cells to biomaterials is a prerequisite for most biomedical applications; e.g., neuroelectrodes can only stimulate brain tissue in vivo if the electric signal is transferred to neurons attached to the electrodes’ surface. Besides, cell survival in vitro also depends on the interaction of cells with the underlying substrate materials; in vitro assays such as multielectrode arrays determine cellular behavior by electrical coupling to the adherent cells. In our study, we investigated the interaction of neurons and glial cells with different electrode materials such as TiN and nanocolumnar TiN surfaces in contrast to gold and ITO substrates. Employing single-cell force spectroscopy, we quantified short-term interaction forces between neuron-like cells (SH-SY5Y cells) and glial cells (U-87 MG cells) for the different materials and contact times. Additionally, results were compared to the spreading dynamics of cells for different culture times as a function of the underlying substrate. The adhesion behavior of glial cells was almost independent of the biomaterial and the maximum growth areas were already seen after one day; however, adhesion dynamics of neurons relied on culture material and time. Neurons spread much better on TiN and nanocolumnar TiN and also formed more neurites after three days in culture. Our designed nanocolumnar TiN offers the possibility for building miniaturized microelectrode arrays for impedance spectroscopy without losing detection sensitivity due to a lowered self-impedance of the electrode. Hence, our results show that this biomaterial promotes adhesion and spreading of neurons and glial cells, which are important for many biomedical applications in vitro and in vivo.     

egoisst: a negotiation support system for electronic business-to-business negotiations in e-commerce

Electronic negotiations can range from simple offer exchanges to complex communicative acts concerning packages of products and services. In contrast to dominant approaches aiming at automating the negotiation process (e.g. auction models), we introduce the notion of negotiation support for human negotiators conducting complex electronic negotiations. The negotiation support system egoisst for business-to-business electronic commerce is presented that is based on theories of communication and information systems and that combines communication and document management. egoisst has been successfully validated for e-negotiations in the construction industry.     

Investigation of natural biofilms formed during the production of drinking water from surface water embankment filtration

Populations of bacteria in biofilms from different sites of a drinking water production system were analysed. Polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis (DGGE) analyses revealed changing DNA band patterns, suggesting a population shift during bank filtration and processing at the waterworks. In addition, common DNA bands that were attributed to ubiquitous bacteria were found. Biofilms even developed directly after UV disinfection (1-2m distance). Their DNA band patterns only partly agreed with those of the biofilms from the downstream distribution system. Opportunistic pathogenic bacteria in biofilms were analysed using PCR and Southern blot hybridisation (SBH). Surface water appeared to have a direct influence on the composition of biofilms in the drinking water distribution system. In spite of preceding filtration and UV disinfection, opportunistic pathogens such as atypical mycobacteria and Legionella spp. were found in biofilms of drinking water, and Pseudomonas aeruginosa was detected sporadically. Enterococci were not found in any biofilm. Bacterial cell counts in the biofilms from surface water to drinking water dropped significantly, and esterase and alanine-aminopeptidase activity decreased. beta-glucosidase activity was not found in the biofilms. Contrary to the results for planktonic bacteria, inhibitory effects were not observed in biofilms. This suggested an increased tolerance of biofilm bacteria against toxic compounds.     

Regulation of CD4+ and CD8+ T Cell Biology by Short-Chain Fatty Acids and Its Relevance for Autoimmune Pathology

The gut microbiota encodes a broad range of enzymes capable of synthetizing various metabolites, some of which are still uncharacterized. One well-known class of microbiota-derived metabolites are the short-chain fatty acids (SCFAs) such as acetate, propionate, butyrate and valerate. SCFAs have long been considered a mere waste product of bacterial metabolism. Novel results have challenged this long-held dogma, revealing a central role for microbe-derived SCFAs in gut microbiota-host interaction. SCFAs are bacterial signaling molecules that act directly on host T lymphocytes by reprogramming their metabolic activity and epigenetic status. They have an essential biological role in promoting differentiation of (intestinal) regulatory T cells and in production of the anti-inflammatory cytokine interleukin-10 (IL-10). These small molecules can also reach the circulation and modulate immune cell function in remote tissues. In experimental models of autoimmune and inflammatory diseases, such as inflammatory bowel disease, multiple sclerosis or diabetes, a strong therapeutic potential of SCFAs through the modulation of effector T cell function was observed. In this review, we discuss current research activities toward understanding a relevance of microbial SCFA for treating autoimmune and inflammatory pathologies from in vitro to human studies.     

Development of Viral-Vectored Vaccines and Virus Replicon Particle-Based Neutralisation Assay against Mayaro Virus

Mayaro virus (MAYV) is an emerging alphavirus causing acute febrile illness associated with chronic polyarthralgia. Although MAYV is currently restricted to tropical regions in South America around the Amazon basin, it has the potential to spread globally by Aedes species mosquitoes. In addition, there are currently no specific therapeutics or licenced vaccines against MAYV infection. We have previously shown that an adenovirus based Mayaro vaccine (ChAdOx1 May) was able to provide full protection against MAYV challenge in vaccinated A129 mice and induced high neutralising antibody titres. In this study, we have constructed a replication deficient simian adenovirus (ChAdOx2) and a Modified Ankara Virus (MVA) based vaccine expressing the MAYV structural cassette (sMAYV) similar to ChAdOx1 May, and characterised recombinant MAYV E2 glycoprotein expressed in a mammalian system for immune monitoring. We demonstrate that ChAdOx2 May was able to induce high antibody titres similar to ChAdOx1 May, and MVA May was shown to be an effective boosting strategy following prime vaccination with ChAdOx1 or ChAdOx2 May. In order to measure MAYV neutralising ability, we have developed a virus replicon particle-based neutralisation assay which effectively detected neutralising antibodies against MAYV. In summary, our study indicates the potential for further clinical development of the viral vectored MAYV vaccines against MAYV infections.     

User Innovation in Techniques: A Case Study Analysis in the Field of Medical Devices

Users are the primary source of new techniques, i.e. systematic activities by which a complex task is accomplished. Even though user‐generated techniques are a pervasive phenomenon which can be observed in many different contexts, existing research on user innovation has focused on product and service innovations. We focus on this important area of user innovation by studying four cases in the field of medical devices to analyse the processes by which users generate and diffuse new techniques as well as the interdependencies between user‐generated techniques and subsequent changes to product use and product innovation. Our findings suggest that user innovation in techniques triggers product innovation by users and manufacturers. Therefore, users' contributions to total innovation output are much higher than previously considered. Additionally, the diffusion of techniques differs significantly from the diffusion of new products. It can only be achieved with the involvement of diffusion agents and their interpersonal interaction and joint performance of the technique with possible adopters. As the diffusion of a technique is often a prerequisite for the diffusion of any related product, companies are forced to re‐think their marketing strategies.