Reconstruction of defects of the trachea

The trachea has a complex anatomy to fulfill its tasks. Its unique fibro-cartilaginous structure maintains an open conduit during respiration, and provides vertical elasticity for deglutition, mobility of the neck and speech. Blood vessels pierce the intercartilaginous ligaments to perfuse the ciliated epithelium, which ensures effective mucociliary clearance. Removal of a tracheal segment affected by benign or malignant disease requires airtight restoration of the continuity of the tube. When direct approximation of both tracheal ends is no longer feasible, a reconstruction is needed. This may occur in recurrent short-segment defects in a scarred environment, or in defects comprising more than half the length of the trachea. The resulting gap must be filled with vascularized tissue that restores the mucosal lining and supports the semi-rigid, semi-flexible framework of the trachea. For long-segment or circular defects, restoration of this unique biomechanical profile becomes even more important. Due to the inherent difficulty of creating such a tube, a tracheostomy or palliative stenting are often preferred over permanent reconstruction. To significantly improve and sustain quality of life of these patients, surgeons proposed innovative strategies for complex tracheal repair. In this review, we provide an overview of current clinical applications of tracheal repair using autologous and allogenic tissues. We look at recent advances in the field of tissue engineering, and the areas for improvement of these first human applications. Lastly, we highlight the focus of our research, in an effort to contribute to the development of optimized tracheal reconstructive techniques.     

<Emphasis Type="Italic">Zea Mays</Emphasis>: Benzoxazolinone Detoxification Under Sulfur Deficiency Conditions—A Complex Allelopathic Alliance Including Endophytic <Emphasis Type="Italic">Fusarium Verticillioides</Emphasis>

Benzoxazolinone detoxification is similar in plants grown under sulfur deficiency conditions and in control plants grown with an optimal S supply. However, when S-deficient plants were treated with metolachlor before benzoxazolin-2(3H)-one (BOA) incubation, detoxification was reduced, as indicated by a lower accumulation of the detoxification products BOA-6-O-glucoside and glucoside carbamate and by a loss of BOA-6-OH glucosyltransfease activity. Root colonizing microorganisms and the endophytic fungus Fusarium verticillioides participated in benzoxazolinone detoxification by converting the compound to 2-acetamidophenol (AAP) or O-hydroxyphenyl malonamic acid (OHPMA), a process accompanied by the appearance of phenoxazinone. Maize roots, however, absorbed AAP and OHPMA only in traces. Absorbed traces of OHPMA stimulated maize radicle growth, and traces of AAP stimulated cress. Phenoxazinone inhibited the growth of cress radicles at concentrations higher than 500 μM, whereas maize radicles were hardly affected. F. verticillioides did not convert benzoxazolinone to any known microbial degradation product when the medium of maize seedlings was inoculated with the fungus under sterile condition. Plant–fungus interactions seem to be important in plant survival of allelopathic attacks. This study points to a complicated network of allelopathic interactions that are influenced by biotic and abiotic factors, including herbicides.     

Estimating postprandial glucose fluxes using hierarchical Bayes modelling

A new stochastic computational method was developed to estimate the endogenous glucose production, the meal-related glucose appearance rate (R(a meal)), and the glucose disposal (R(d)) during the meal tolerance test. A prior probability distribution was adopted which assumes smooth glucose fluxes with individualized smoothness level within the context of a Bayes hierarchical model. The new method was contrasted with the maximum likelihood method using data collected in 18 subjects with type 2 diabetes who ingested a mixed meal containing [U-(13)C]glucose. Primed [6,6-(2)H(2)]glucose was infused in a manner that mimicked the expected endogenous glucose production. The mean endogenous glucose production, R(a meal), and R(d) calculated by the new method and maximum likelihood method were nearly identical. However, the maximum likelihood gave constant, nonphysiological postprandial endogenous glucose production in two subjects whilst the new method gave plausible estimates of endogenous glucose production in all subjects. Additionally, the two methods were compared using a simulated triple-tracer experiment in 12 virtual subjects. The accuracy of the estimates of the endogenous glucose production and R(a meal) profiles was similar [root mean square error (RMSE) 1.0±0.3 vs. 1.4±0.7μmol/kg/min for EGP and 2.6±1.0 vs. 2.9±0.9μmol/kg/min for R(a meal); new method vs. maximum likelihood method; P=NS, paired t-test]. The accuracy of R(d) estimates was significantly increased by the new method (RMSE 5.3±1.9 vs. 4.2±1.3; new method vs. ML method; P<0.01, paired t-test). We conclude that the new method increases plausibility of the endogenous glucose production and improves accuracy of glucose disposal compared to the maximum likelihood method.     

Laser-ablated volume and depth as a function of pulse duration in aluminum targets

The ablated depth and volume per laser pulse from an aluminum target were measured for pulse durations that ranged from 80 fs to 270 ps at an average fluence of approximately 100 J/cm2 and a wavelength of 0.8 microm. The ablated volume showed a flat maximum for subpicosecond pulses and a minimum for approximately 6 ps. The crater diameters were rather constant up to pulse durations of approximately 6 ps and increased for larger pulse durations. As a result, the ablated depth also showed a plateau for subpicosecond pulses but decreased monotonically with pulse duration. A physical interpretation of these results and their consequences for laser applications are discussed.     

Investigation of the state of local thermodynamic equilibrium of a laser-produced aluminum plasma

In this work, the assumption of local thermodynamic equilibrium (LTE) for a laser-induced plasma in ambient air is examined experimentally using two different laser systems, namely an infrared short-pulse Ti : Sapphire laser and an ultraviolet long-pulse XeCl excimer laser. The LTE assumption is investigated by examining the plasma produced at a laser fluence of 10 J/cm(2) from aluminum targets containing iron and magnesium impurities. The excitation temperature is deduced from Boltzmann diagrams built from a large number of spatially integrated neutral iron lines distributed from 3.21 to 6.56 eV. It is shown that at any time after the end of the laser pulse, the neutral excited states are in excellent Boltzmann equilibrium. Detailed investigation of Boltzmann equilibrium further validates previous temperature measurements using less accurate diagrams. However, observations of ion lines provide some evidence that the ionized species do not obey Saha equilibrium, thereby indicating departure from LTE. This could be explained by the fact that the plasma cannot be considered as stationary for these species.     

Sequential unfolding of novelty and pleasantness appraisals of odors: Evidence from facial electromyography and autonomic reactions.

We investigated the effects of odors on appraisal processes and consequent emotional responses. The main goal was to test whether an odor is detected as novel or familiar before it is evaluated as pleasant or unpleasant. Participants performed a recognition task in which they were presented with pairs of unpleasant or pleasant odors (sample and target odors). Within a pair, the sample and target were either identical or different to assess participants’ novelty detection; unpleasant and pleasant target odors were contrasted to examine participants’ appraisal of intrinsic pleasantness. We measured facial expressions using electromyography and physiological reactions using electrocardiogram and electrodermal activity in response to odors. The earliest effects on facial muscles and heart rate occurred in response to novelty detection. Later effects on facial muscles and heart rate were related to pleasantness evaluation. This study is the first to demonstrate the existence of a sequence of appraisal checks for odors eliciting emotional reaction. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Conservation in PR China

Chinese planners have recently changed the policy towards historic cities and sites. After the Communist victory in 1949 the housing problem was emphasized, especially during the first period under Soviet influence and during the Cultural Revolution. Historic buildings, characteristic clay-and-straw houses and other memorials of the imperial and colonial past seemed elements to be eliminated from modern society. However, after the fall of the Gang of Four in 1976, the history of the people became an important issue. This article, based on a recent study tour to PR China, offers a personal view of the development of the conservation movement in China.     

Chemische Untersuchungen an Speiseölen unter besonderer Berücksichtigung polyensäurereicher Öle

Analysis of Edible Oils with Special Consideration of Oils Containing Polyunsaturated Fatty Acids 32 different edible oils including several oils containing unsaturated fatty acids were controlled with reference to quality and oxidation state by determination of acid content, saponification value, peroxide value, thiobarbituric acid value, volatile substances and smoke point before and after heating. Except for the fact that heating always causes a reduction of quality of oils, native olive oils and sunflower oils proved to be very susceptible for oxidation whereas refined “table” oils and corn oils showed higher stability.