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131.
Yagüe J Paradela A Ramos M Ogueta S Marina A Barahona F López de Castro JA Vázquez J 《Analytical chemistry》2003,75(6):1524-1535
The emergence of proteomics has placed great interest in the understanding of the mechanisms of MS/MS fragmentation of peptides under low-energy collision-induced dissociation. In this work, we describe the presence of anomalous fragments, which correspond to neutral loss elimination of internal amino acids from ions of the b series in quadrupole ion trap MS/MS spectra from naturally occurring peptides. Internal amino acid elimination occurred preferentially with aliphatic amino acids. The phenomenon was more apparent when doubly charged precursors were fragmented and was inhibited when peptides were N-acetylated at the N-terminus. Fragmentation of isomeric peptides where some internal amino acids were relocated in N-terminal position produced MSn spectra indistinguishable from those of the original peptides, indicating that some b ions underwent a structural rearrangement process. Formation of anomalous fragments required a minimum activation time. Our data are consistent with a nucleophile attack of the N-terminal nitrogen over the electrophilic carbonyl carbon at one peptide bond, forming a cyclic b ion intermediate that, by reopening at preferential sites, exposes internal amino acids to the C-terminal side. 相似文献
132.
We compare the three basic algorithms for model-based clustering on high-dimensional discrete-variable datasets. All three algorithms use the same underlying model: a naive-Bayes model with a hidden root node, also known as a multinomial-mixture model. In the first part of the paper, we perform an experimental comparison between three batch algorithms that learn the parameters of this model: the Expectation–Maximization (EM) algorithm, a winner take all version of the EM algorithm reminiscent of the K-means algorithm, and model-based agglomerative clustering. We find that the EM algorithm significantly outperforms the other methods, and proceed to investigate the effect of various initialization methods on the final solution produced by the EM algorithm. The initializations that we consider are (1) parameters sampled from an uninformative prior, (2) random perturbations of the marginal distribution of the data, and (3) the output of agglomerative clustering. Although the methods are substantially different, they lead to learned models that are similar in quality. 相似文献
133.
Wireless Personal Communications - 相似文献
134.
The dielectric properties of a polydomain triglycine sulfate (TGS) crystal near the Curie temperature (20 Hz to 20 kHz) were compared with those of monodomain crystals (containing -alanine impurity or gamma-irradiated). The frequency and temperature dependences of ac conductivity were used to assess its effect on the dielectric properties of TGS nearT
C. The crystals were found to differ in the variation of the real part of permittivity with temperature, which was tentatively attributed to the difference in the density of domain walls in the paraelectric phase near T
C. 相似文献
135.
Wilson Keith G.; Chochinov Harvey Max; McPherson Christine J.; Skirko Merika Graham; Allard Pierre; Chary Srini; Gagnon Pierre R.; Macmillan Karen; De Luca Marina; O'Shea Fiona; Kuhl David; Fainsinger Robin L.; Karam Andrea M.; Clinch Jennifer J. 《Canadian Metallurgical Quarterly》2007,26(3):314
Objective: To investigate the attitudes of terminally ill individuals toward the legalization of euthanasia or physician-assisted suicide (PAS) and to identify those who would personally desire such a death. Design: In the Canadian National Palliative Care Survey, semistructured interviews were administered to 379 patients who were receiving palliative care for cancer. Patients who expressed a desire for physician-hastened death were followed prospectively. Main Outcome Measures: Attitudes toward the legalization of euthanasia or PAS were determined, as was the personal interest in receiving a hastened death. Demographic and clinical characteristics were also recorded, including a 22-item structured interview of symptoms and concerns. Results: There were 238 participants (62.8%) who believed that euthanasia and/or PAS should be legalized, and 151 (39.8%) who would consider making a future request for a physician-hastened death. However, only 22 (5.8%) reported that, if legally permissible, they would initiate such a request right away, in their current situations. This desire for hastened death was associated with lower religiosity (p = .010), reduced functional status (p = .024), a diagnosis of major depression (p 相似文献
136.
Eakin Elizabeth G.; Bull Sheana S.; Riley Kimberley M.; Reeves Marina M.; McLaughlin Patty; Gutierrez Silvia 《Canadian Metallurgical Quarterly》2007,26(4):392
Objective: The Resources for Health trial evaluates a social-ecologically based lifestyle (physical activity and diet) intervention targeting low-income, largely Spanish-speaking patients with multiple chronic conditions. Design: A randomized controlled trial was conducted with 200 patients recruited from an urban community health center and assigned to intervention and usual care conditions. Intervention involved 2 face-to-face, self-management support and community linkage sessions with a health educator, 3 follow-up phone calls, and 3 tailored newsletters. Main Outcome Measures: Primary outcomes measured at 6-months were changes in dietary behavior and physical activity. Changes in multilevel support for healthy living were evaluated as a secondary outcome. Results: After adjustment for age, sex, language, and number of chronic conditions, significant intervention effects were observed for dietary behavior and multilevel support for healthy lifestyles but not for physical activity. Conclusion: The Resources for Health intervention provides an effective and practical model for improving health behavior among low-income, Spanish-speaking patients with multiple chronic conditions. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
137.
Silvia Angelone Marina Cauhapé Casaux Manuel Borghi Fernando O. Martinez 《Materials and Structures》2016,49(5):1655-1665
Nowadays, the disposal of plastic waste is an issue of major concern worldwide because of its considerable volume and growth. An option to tackle this problem is to recycle this waste. This alternative reduces the quantity of net discards, conserves both material and energy and provides a comparatively simple way to make a substantial reduction in the overall volume of solid waste. The purpose of this study is to investigate an environmentally friendly approach about the influence of recycling different percentages of urban and rural plastic waste by adding them in a dry process on an asphalt mixture, through a comparative laboratory study. The resulting mixtures are analyzed considering their volumetric parameters and the values from diverse laboratory mechanical tests. The performance tests include, Marshall stability, Marshall quotient, indirect tensile strength, fracture energy, resilient modulus, permanent deformation and creep compliance, which were carried out on unmodified and modified hot asphalt mixtures. The obtained results are presented and discussed in this paper showing that the reuse of recycled plastics in asphalt mixtures is a viable alternative that contributes to the reduction of plastic wastes as well as the protection of the environment. 相似文献
138.
In this work, we examine the classic problem of robot navigation via visual simultaneous localization and mapping (SLAM), but introducing the concept of dual optical and thermal (cross-spectral) sensing with the addition of sensor handover from one to the other. In our approach we use a novel combination of two primary sensors: co-registered optical and thermal cameras. Mobile robot navigation is driven by two simultaneous camera images from the environment over which feature points are extracted and matched between successive frames. A bearing-only visual SLAM approach is then implemented using successive feature point observations to identify and track environment landmarks using an extended Kalman filter (EKF). Six-degree-of-freedom mobile robot and environment landmark positions are managed by the EKF approach illustrated using optical, thermal and combined optical/thermal features in addition to handover from one sensor to another. Sensor handover is primarily targeted at a continuous SLAM operation during varying illumination conditions (e.g., changing from night to day). The final methodology is tested in outdoor environments with variation in the light conditions and robot trajectories producing results that illustrate that the additional use of a thermal sensor improves the accuracy of landmark detection and that the sensor handover is viable for solving the SLAM problem using this sensor combination. 相似文献
139.
Alexander Kromka Marina Davydova Bohuslav Rezek Milan Vanecek Martin Stuchlik Petr Exnar Martin Kalbac 《Diamond and Related Materials》2010,19(2-3):196-200
Toxic gas sensing device with metal electrodes built into nanocrystalline diamond (NCD) is investigated. The NCD morphology is controlled via seeding and/or deposition time. The surface properties and morphology of NCD are studied using scanning electron microscopy (SEM) and atomic force microscopy (AFM). AFM measurements reveal increase in NCD surface area by up to 13%. Gas sensing properties of H-terminated NCD device show high sensitivity towards oxidizing species where the surface conductivity is increased by an order of magnitude for humid air and by three orders of magnitude for COCl2. The surface conductivity exhibits a small decrease to reducing spices (CO2, NH3). 相似文献
140.
A culture-independent and two culture-dependent real time (rt-) PCR approaches were developed to quantitatively identify Listeria monocytogenes in raw milk and soft cheeses. The optimised rt-PCR revealed 100% inclusivity and exclusivity. DNA- and cell-based standard curves showed a good linearity of response (R2 ≥ 0.987 and R2 ≥ 0.998, respectively) for five orders of magnitude (39 × 105 – 3 × 100 genome equivalents and 106–101 CFU equivalents, respectively) with about 100% relative accuracy and inter-assay variability ≤0.90%. Up to 1 genome equivalent/and 10 CFU/reaction were quantified in the DNA and cell standard curves, respectively. The rt-PCR was then combined with a liquid- (MPN technique) or a solid- (ALOA and PALCAM) based enumeration. The diagnostic sensitivity of the different approaches was investigated in artificially contaminated raw milk and soft cheeses. The rt-PCR culture-independent approach performed well in raw milk and (with a lower sensitivity) in stracchino cheese-based standard curves. MPN/rt-PCR was the best approach to enumerate low levels of L. monocytogenes in raw milk and stracchino cheese, while the ALOA-based rt-PCR quantification was more effective than the PALCAM-based. These performances were confirmed when 23 real samples of raw milk and soft cheeses by both the rt-PCR approaches were assayed. 相似文献