Metabolic behavior in rats of a nonprotein microemulsion resembling low-density lipoprotein

A protein-free microemulsion (LDE) with a lipid composition resembling that of low-density lipoprotein (LDL) was used in metabolic studies in rats to compare LDE with the native lipoprotein. LDE labeled with radioactive lipids was injected into the bloodstream of male Wistar rats, and plasma kinetics of the labeled lipids were followed on plasma samples collected at regular intervals for 12 h after injection. The 24-h LDE uptake by different tissues was also measured in tissue samples excised after the animals had been sacrificed. We found that LDE plasma kinetics were similar to those described for native LDL [fractional clearance rate (FCR) of cholesteryl ester, 0.42±0.11 h⁻¹]. The major site for LDE uptake was the liver, and the tissue distribution of the LDE injected radioactivity was as one would expect for LDL. To test whether LDE was taken up by the specific LDL receptors, the LDE emulsion was injected into rats treated with 17α-ethinylestradiol, which is known to increase the activity of these receptors; as expected, removal of LDE from the bloodstream increased (FCR=0.90±0.35 h⁻¹). On the other hand, saturation of the receptors that remove remnants by prior infusion of massive amounts of lymph chylomicrons did not change LDE plasma kinetics. These results indicate that LDE is cleared from plasma by B,E receptors and not by the E receptors that remove remnants. Incorporation of free cholesterol into LDE increased LDE plasma clearance. Incubation studies also showed that LDE incorporates a variety of apolipoproteins, including apo E, a ligand for recognition of lipoproteins by specific receptors. Our data suggest that LDE can be a useful tool to test LDL metabolism and B,E receptor function.     

1H nuclear magnetic resonance study of polyurethane prepolymers from toluene diisocyanate and polypropylene glycol

Polyurethane prepolymers prepared from toluene 2,4‐diisocyanate, toluene 2,6‐diisocyanate, and polypropylene glycol with a ratio between the isocyanate and hydroxyl groups equal to 2 were analyzed by ¹H nuclear magnetic resonance (NMR) spectroscopy in acetone‐d₆. Different temperatures and concentrations were used. Toluene 2,4‐dimethylurethane and toluene 2,6‐dimethylurethane were synthesized and used as model compounds to assign prepolymers signals. Measurements of spin–lattice relaxation time T₁ by “inversion recovery” experiments were carried out on toluene 2,4‐diisocyanate, toluene 2,6‐diisocyanate, toluene 2,4‐dimethylurethane, toluene 2,6‐dimethylurethane, and polyurethane prepolymers. Differences in T₁ times were used to interpret prepolymers spectra, by means of the strong observed effect on protons due to the presence of adjacent isocyanate groups. © 2002 Wiley Periodicals, Inc. J Appl Polym Sci 87: 347–357, 2003     

X-ray crystal structure of the dye 2-cyano-4-bromo-4′-N,N-diethylaminoazobenzene

The crystal structure and molecular conformation of 2-cyano-4-bromo-4′-N,N-diethylaminoazobenzene (C₁₇H₁₇N₄Br, mol. wt. = 357·2 a.m.u) has been determined from X-ray diffraction data; triclinic, P$\text{1}$ (No. 2), a = 10·132(11) Å, b = 12·216(16) Å, c = 6·966(11) Å, α = 104·21(9)°, β = 92·67(12)°, γ = 97·22(7)°, V = 826·5(9) ų, Z = 2, D_c = 1·436 g cm^?3, F(000) = 378, λ(MoKα) = 0·71069 Å, μ(MoKα) = 26·0 cm^?1. The structure was solved by the multiple solution direct method and refined by full-matrix least-squares to R = 0·059 for 1538 independent observed reflections. The azobenzene skeleton is planar to within 0·06 Å. Most significant bonding data are: NN, 1·290(8) Å; BrC, 1·866(6) Å; mean CN (azo) 1·380(8) Å; NNC, 113·6(4) and 115·3(4)°; NCC (cis relative to NN) 125·9(4)° and 126·7(4)°; NCC (trans) 116·8°(5)° and 116·1(4)°.     

Breaking Bad in Cyberspace: Understanding why and how Black Hat Hackers Manage their Nerves to Commit their Virtual Crimes

Information Systems Frontiers - What is happening in hacker’s minds when they are committing criminal activities? How black hat hackers manage nerves, which is about managing fear and...     

A scalable solution to the nearest neighbor search problem through local-search methods on neighbor graphs

Pattern Analysis and Applications - Nearest neighbor search is a powerful abstraction for data access; however, data indexing is troublesome even for approximate indexes. For intrinsically...     

The Resource-Energy Nexus as a Key Factor for Circular Economy

The extraction of raw materials is associated with energy input and CO₂ emissions. What is obvious for extraction from mining, however, also applies to recycling. Mostly, recycling is preferred for reasons of climate protection or because of the geological scarcity of raw materials, which is controversially discussed. While in mining, the declining ore grade is a driver for the energy demand, in case of recycling it is the dissipation of materials into products or waste. As concentration decreases, the effort required also increases disproportionately. The “closing the loop” metaphor of Circular Economy is therefore inappropriate in its stricter meaning. It is rather about optimizing the overall system and finding the optimal recycling rate. However, first, it must be clarified what the political goals for Circular Economy are.     

Compatibilization of an immiscible blend of EPDM and POM with an Ionomer

Immiscible blends of ethylene-propylene-diene-monomer (EPDM) and polyoxymethylene (POM), when EPDM is the major phase were compatibilized on the addition of an ionomer, poly(ethylene-co-methacrylic acid). The inclusion of the ionomer reduced the interfacial tension between the two phases, such that the diameter of the POM domains were significantly reduced to between 0.5 and 2 μm, typical of that required to toughen ductile polymers. The mechanical properties of the resultant compatibilized blends were significantly enhanced with increases in Young's modulus (↑54%), tensile strength (σ, ↑139%), elongation at break (ε, ↑97%), and tensile toughness (↑500%) with increasing ionomer content, relative to EPDM rubber alone. The ShoreA hardness of the compatibilized blend was 70.1 compared with 56.8 for the immiscible binary blend and, 50.2 for neat EPDM rubber.     

The Nyquist stability criterion in the Nichols chart

The Nyquist stability criterion is a widely used technique for determining in the complex s‐plane the stability of a dynamical system with feedback. This paper presents a practical and comprehensive method to compute the Nyquist stability criterion directly in the Nichols (magnitude/phase) chart. The proposed method also gives guidelines to design controllers to stabilize unstable plants when dealing with frequency domain techniques like the quantitative feedback theory robust control. Copyright © 2015 John Wiley & Sons, Ltd.     

Parallel simulation of Population Dynamics P systems: updates and roadmap

Population Dynamics P systems are a type of multienvironment P systems that serve as a formal modeling framework for real ecosystems. The accurate simulation of these probabilistic models, e.g. with Direct distribution based on Consistent Blocks Algorithm, entails large run times. Hence, parallel platforms such as GPUs have been employed to speedup the simulation. In 2012, the first GPU simulator of PDP systems was presented. However, it was able to run only randomly generated PDP systems. In this paper, we present current updates made on this simulator, involving an input modu le for binary files and an output module for CSV files. Finally, the simulator has been experimentally validated with a real ecosystem model, and its performance has been tested with two high-end GPUs: Tesla C1060 and K40.     

Performance bounds in linear control of unstable MIMO systems with pole location constraint

This paper proposes a methodology to compute quadratic performance bounds when the closed loop poles of a discrete-time multivariable control loop are confined to a disk, centred at the origin, and with radius less than one. The underlying philosophy in this constraint is to avoid certain undesirable dynamic features which arise in quadratic optimal designs. An expression for the performance loss due to the pole location constraint is also provided. Using numerical examples, we show that the performance loss is compensated by an improved transient performance, specially visible in the control signals.