3D true-phase polarity recovery with independent phase estimation using three-tier stacks based region growing (3D-TRIPS)

Objectives

A postprocessing technique termed 3D true-phase polarity recovery with independent phase estimation using three-tier stacks based region growing (3D-TRIPS) was developed, which directly reconstructs phase-sensitive inversion-recovery images without acquisition of phase-reference images. The utility of this technique is demonstrated in myocardial late gadolinium enhancement (LGE) imaging.

Materials and methods

A data structure with three tiers of stacks was used for 3D-TRIPS to directly achieve reliable region growing for successful background-phase estimation. Fifteen patients undergoing postgadolinium 3D phase-sensitive inversion recovery (PSIR) cardiac LGE magnetic resonance imaging (MRI) were recruited, and 3D-TRIPS LGE reconstructions were compared with standard PSIR. Objective voxel-by-voxel comparison was performed. Additionally, blinded review by two radiologists compared scar visibility, clinical acceptability, voxel polarity error, or groups and blurring.

Results

3D-TRIPS efficiently reconstructed postcontrast phase-sensitive myocardial LGE images. Objective analysis showed an average 95% voxel-by-voxel agreement between 3D-TRIPS and PSIR images. Blinded radiologist review demonstrated similar image quality between 3D-TRIPS and PSIR reconstruction.

Conclusion

3D-TRIPS provided similar image quality to PSIR for phase-sensitive myocardial LGE MRI reconstruction. 3D-TRIPS does not require acquisition of a reference image and can therefore be used to accelerate phase-sensitive LGE imaging.

     

X‐ray micrographic imaging system based on COTS CMOS sensors

This paper presents the use of commercial off the shelf CMOS image sensors for the acquisition of X‐ray images with high spatial resolution. The X‐ray images, with application in biology, electronic components inspection, and paleontology research, are obtained with 8‐keV photons from a Cu tube. The quantum efficiency of the detector is estimated using attenuation lengths of photons in the sensor and compared to traditional scintillator conversion layers. The spatial resolution observed with the sensor is limited by the charge redistribution produced after photon interaction with Si.     

Separable identification of nonlinear aggregate power system loads

An identification algorithm for a power system load model is proposed in this paper. The overall non-convex identification problem is separated into convex and non-convex subproblems, allowing for a global optimum to be found.     

Illumination with solid state lighting technology

High-power light-emitting diodes (LEDs) have begun to differentiate themselves from their more common cousins the indicator LED. Today these LEDs are designed to generate 10-100 lm per LED with efficiencies that surpass incandescent and halogen bulbs. After a summary of the motivation for the development of the high-power LED and a look at the future markets, we describe the current state of high-power LED technology and the challenges that lay ahead for development of a true "solid state lamp." We demonstrate record performance and reliability for high-power colored and white LEDs and show results from the worlds first 100-plus lumen white LED lamp, the solid state equivalent of Thomas Edison's 20-W incandescent lightbulb approximately one century later     

国内高端品牌市场特征与发展机遇

“可承受类高端消费品”是近年来时尚产业高速发展下出现的新名词,即指集高级材料、精湛做工和优秀设计为一体的、价格能为相对广泛的中高收入阶层所承受的优质时尚产品。这种产品既区别于受众极少的顶级奢侈品,也不同于普通消费阶层购买的低端产品。而眼镜产品也隶属于可承受类奢侈品的范围,这也是此次金融危机的重灾区。     

Computing Nash Equilibria for Scheduling on Restricted Parallel Links

We consider the problem of routing n users on m parallel links under the restriction that each user may only be routed on a link from a certain set of allowed links for the user. So, this problem is equivalent to the correspondingly restricted scheduling problem of assigning n jobs to m parallel machines. In a Nash equilibrium, no user may improve its own Individual Cost (latency) by unilaterally switching to another link from its set of allowed links.     

Self-diffusion in Solutions of Carboxylated Acrylic Polymers as Studied by Pulsed Field Gradient NMR. 1. Solvent Diffusion Studies

The diffusion constants of solvent in solutions of carboxylated acrylic random copolymers in isopropanol (IPA) have been investigated using the PFG NMR technique. The copolymers are composed of butyl methacrylate and methacrylic acid with a range of molar ratios (BMA/MAA 100/0 through to 60/40 in 10 mol% steps). It was found that the echo attenuation function depended on the diffusion time, and experiments with short and long diffusion times have been analysed separately. Two questions are addressed: the first concerns the influence of concentration on the polymer aggregation in solution, and the second concerns the influence of the BMA/MAA molar ratio on the diffusion constants of the solvent. The data obtained have been discussed using established physical models for diffusion. Using the model of Wang gave the relative mass of bound solvent per mass of polymer which increased with the mol% of MAA in the copolymer.     

Oleoyl‐ethanolamide (OEA): A bioactive lipid derived from oleic acid and phosphatidylethanol‐amine

Fatty acid ethanolamides are lipid compounds with biological activities. Among them, oleoyl‐ethanolamide (OEA) has attracted a great deal of interest because of its biological properties. OEA is found in low amount in foodstuffs, but mainly stems from endogenous synthesis. In rodents, intraperitoneal administration of OEA induces satiety and peripheral utilization of lipid substrate. Both in vitro studies and knock‐out animal models have revealed some mechanisms of action, such as PPARα signaling, selected neuron activation and endocrine (ghrelin) signaling. The proximal intestine seems to be a target organ for satiety control. Acute oral administration also induces satiety. Further studies are still required to better address the physiological impact of both oral and intraperitoneal OEA when administrated in the long term.     

Synthesis and In Vitro Evaluation of C-7 and C-8 Luteolin Derivatives as Influenza Endonuclease Inhibitors

The part of the influenza polymerase PA subunit featuring endonuclease activity is a target for anti-influenza therapies, including the FDA-approved drug Xofluza. A general feature of endonuclease inhibitors is their ability to chelate Mg²⁺ or Mn²⁺ ions located in the enzyme’s catalytic site. Previously, we screened a panel of flavonoids for PA inhibition and found luteolin and its C-glucoside orientin to be potent inhibitors. Through structural analysis, we identified the presence of a 3′,4′-dihydroxyphenyl moiety as a crucial feature for sub-micromolar inhibitory activity. Here, we report results from a subsequent investigation exploring structural changes at the C-7 and C-8 positions of luteolin. Experimental IC₅₀ values were determined by AlphaScreen technology. The most potent inhibitors were C-8 derivatives with inhibitory potencies comparable to that of luteolin. Bio-isosteric replacement of the C-7 hydroxyl moiety of luteolin led to a series of compounds with one-order-of-magnitude-lower inhibitory potencies. Using X-ray crystallography, we solved structures of the wild-type PA-N-terminal domain and its I38T mutant in complex with orientin at 1.9 Å and 2.2 Å resolution, respectively.     

Photocaged Hoechst Enables Subnuclear Visualization and Cell Selective Staining of DNA in vivo

Selective targeting of DNA by means of fluorescent labeling has become a mainstay in the life sciences. While genetic engineering serves as a powerful technique and allows the visualization of nucleic acid by using DNA-targeting fluorescent fusion proteins in a cell-type- and subcellular-specific manner, it relies on the introduction of foreign genes. On the other hand, DNA-binding small fluorescent molecules can be used without genetic engineering, but they are not spatially restricted. Herein, we report a photocaged version of the DNA dye Hoechst33342 (pcHoechst), which can be uncaged by using UV to blue light for the selective staining of chromosomal DNA in subnuclear regions of live cells. Expanding its application to a vertebrate model organism, we demonstrate uncaging in epithelial cells and short-term cell tracking in vivo in zebrafish. We envision pcHoechst as a valuable tool for targeting and interrogating DNA with precise spatiotemporal resolution in living cells and wild-type organisms.