Prevalence and risk factors for Salmonella and Campylobacter spp. carcass contamination in broiler chickens slaughtered in Quebec, Canada

An observational study was conducted to estimate prevalence and risk factors for Salmonella and Campylobacter spp. carcass contamination in broiler chickens. Eighty-two lots were sampled in four slaughterhouses located in the province of Québec, Canada, over a 10-month period. Carcass contamination was evaluated by the carcass rinse technique for about 30 birds per lot. Exposure to potential risk factors was evaluated based on data from questionnaires, meteorology, and cecal cultures. Multivariable binomial negative regression models were used for risk factor analysis at the lot level. The prevalence of Salmonella-positive carcasses was 21.2% (95% confidence interval: 15.7 to 26.7%). Significant risk factors (P < 0.05) associated with a higher proportion of positive carcasses within lots were Salmonella-positive cecal culture, low rainfall during transportation to the slaughterhouse, temperature of > or = 0 degree C during transportation to the slaughterhouse, and a > or = 4-h waiting period in shipping crates before slaughtering. The prevalence of Campylobacter-positive carcasses was 35.8% (95% confidence interval: 27.1 to 44.5%). Lots containing birds with Campylobacter-positive cecal culture results, lots of birds that were slaughtered at the end of the week, and lots with at least 20% of birds with digestive contents detected in the jejunum at time of slaughtering had a significantly higher proportion (P < 0.05) of contaminated carcasses. These results support the importance of preharvest control measures implemented during rearing to reduce contamination of the final product. Weather during transportation to slaughter and the day of the week that birds were slaughtered also were associated with carcass contamination; further studies are needed to determine the underlying mechanisms by which these factors influence carcass contamination.     

Effect of natural and controlled fermentation on flatus‐producing compounds of beans (Phaseolus vulgaris)

Fermentation of grain legumes is an efficient method to reduce the concentration of α‐galactosidic compounds that are known to be flatulence producers. Soluble dietary fibre has also been implicated in flatulence production; however, little information exists about the effectiveness of fermentation in diminishing the effects of these compounds. The objective of this work was to study the effect of natural fermentation (NF) and controlled fermentation (CF) on the content of α‐galactosides and dietary fibre in dry beans (Phaseolus vulgaris) for 24, 48, 72 and 96 h. After 48 h, the pH during NF dropped from 6.15 to 4.00 and the nominal acidity increased six times; for CF, however, although the decrease in pH was similar to that for NF, the nominal acidity increased only three times after 48 h. Insoluble fibre content did not change the pH significantly after 96 h for NF and CF. Soluble fibre suffered an apparent removal after 48 h of NF and underwent a sharp reduction of 66% after 96 h of CF. The concentration of stachyose (the main α‐galactoside in raw beans) diminished notably after 48 h and 96 h NF (72% and 95% respectively), whereas with CF only 11% was removed after 96 h. NF of P vulgaris seems to be more effective than CF in reducing the flatulence‐producer factors (α‐galactosides and soluble dietary fibre). Copyright © 2003 Society of Chemical Industry     

Modulation of Starch Digestibility in Breakfast Cereals Consumed by Subjects with Metabolic Risk: Impact on Markers of Oxidative Stress and Inflammation during Fasting and the Postprandial Period

Scope : Decreasing postprandial glycaemic excursions may have a beneficial effect on inflammatory and oxidative stress profiles. In this study, we investigated the impact of carbohydrate digestibility modulation per se, as a means of reducing the glycaemic response, on metabolic and inflammatory responses in subjects with metabolic risk factors. Methods and results : Twenty healthy subjects with metabolic risk consumed a cereal product either high in Slowly Digestible Starch (HSDS) or low in SDS (LSDS) at breakfast daily for 3 weeks, in a cross‐over design. Following each 3‐week session, postprandial glycaemia, insulinaemia, the lipid profile, inflammation and oxidative stress markers were assessed and compared to those induced by ingestion of a glucose solution (as a reference). The 2‐h glycaemic and insulinaemic responses were significantly lower following the HSDS breakfast compared with the LSDS breakfast or glucose. No significant differences between the products were observed in terms of the lipid profile, C‐reactive protein, IL‐6 and tumour necrosis factor alpha. We observed a slight increase in fasting lipid peroxidation markers, including an increase in plasma malondialdehyde (MDA) and a decrease in whole blood glutathione (GSH), without significant alteration of urinary F2‐isoprostanes or plasma glutathione peroxidase (GPx) activity. Conclusion : Consumption of HSDS products for 3 weeks significantly altered both postprandial glycaemia and insulinaemia, but was not sufficient to modify the inflammatory profile. Consumption of both cereal products was associated with a slightly higher fasting oxidative stress profile.     

Preparation of a hydrophobic recycled jute-based nonwoven using a titanium dioxide/stearic acid coating

This work aims at developing a hydrophobic treatment for jute fiber-based nonwovens. Three solutions of titanium dioxide (TiO₂) nanoparticles were prepared through a sol–gel method by varying the molar ratio of the various constituents. The nonwoven was pretreated with these solutions before being impregnated with different concentrations of stearic acid. The TiO₂ nanoparticles synthesized are amorphous; their size varies with the concentration of ethanol used as a solvent in the sol–gel method. The nanoparticle coating produced on the jute fibers is uniform. The nonwoven wettability was evaluated by measuring its water contact angle and retention time; the nonwoven became hydrophobic at the lowest fatty acid concentration tested. An increase in the stability of the hydrophobicity was observed when the TiO₂ nanoparticle pretreatment was used compared to the application of the stearic acid treatment only. No detrimental effect of the hydrophobic treatment on the nonwoven mechanical performance and thermal stability was observed. These results demonstrate the potential of the TiO₂ nanoparticle/stearic acid treatment as a fast method to provide a stable hydrophobicity to recycled jute-based nonwovens.     

Comparison of two models for the estimation of usual intake addressing zero consumption and non-normality

Various models exist for estimating the usual intake distribution from dietary intake data. In this paper, we compare two of these models, the Iowa State University Foods (ISUF) model and the betabinomial-normal (BBN) model and apply them to three different datasets. Intake data are obtained by aggregating over multiple food products and are often non-normal. The ISUF and BBN model both address non-normality. While the two models have similar structures, they show some differences. The ISUF model includes an additional spline transformation for improving the normality of the intake amount distribution, while the BBN model includes the possibility of addressing covariates, such as age or sex. Our analyses showed that for two of the example datasets both models produced similar estimates of the higher percentiles of the usual intake distribution. However, for the third dataset, where the intake amount distribution appear to be multimodal, both models produced different percentile estimates.     

Species identification of meat products by ELISA

ELISA methods used in this study are proved to detect low contents of animal species (pork, beef, sheep and poultry), even in highly processed foods. They present the advantages of being robust, cheap and easy to perform. Nevertheless, F factors, determining the threshold values of the test, need to be validated for each species.     

Analyse du comportement d’un sol argileux sous sollicitations hydriques cycliques

Expansive soils swell and shrink regularly when subjected to moisture changes. Clayey soils are available worldwide and are a continual source of concern causing substantial damage to civil engineering structures. Cyclic expansion and shrinkage of clays and associated movements of foundations may result in cracking and fatigue to structures. In France, the damage caused by this phenomenon was estimated to be more than 3.3 billion euros in 2002 (Vincent in 3^ème conférence SIRNAT-Forum des journées pour la Prévention des Risques Naturels, Orléans, janv. 2003) and the Paris region is one of the most affected. The objective of this study is to investigate the swell–shrink behaviour of a natural clayey soil considered to be responsible for a lot of damage observed on buildings in the Paris region, and thus contributing to the characterisation and understanding of expansive clayey soils. The studied soil, Argile verte de Romainville, is a lagoonal-marine deposit and is part of the Paris Basin Tertiary (Oligocene) formations (Fig. 1). It is a clayey soil sampled in the eastern region of Paris. The mineralogical and geotechnical properties of the soil are presented in Table 1. The soil contains quartz (15–20%), carbonates (12–20%) and traces of mica and feldspars. X-ray diffraction showed that carbonates are essentially dolomite and the clay minerals are dominantly illite, kaolinite and a small amount of smectite (Fig. 2). A grain size analysis shows that the clay content (<2 μm) varies between 78 and 80%. The study of its microstructure by means of the scanning electron microscope indicates that the clayey soil has structural elements oriented in the direction of bedding. The structure of the sample generally consisted of dense and continuous clay matrices with very limited visible pore spaces (Fig. 3). At its natural water content (w = 25%), the soil shows mainly a unimodal pore size distribution with an average pore radius of 0.07 μm and a very limited porosity with radii larger than 10 μm (Fig. 4). To assess the effect of suction on the simultaneous changes in void ratio and degree of saturation under zero external stresses, drying–wetting tests are performed on the natural samples. The osmotic technique (Polyethylene glycol solutions) and various salt solutions are used to control the suction values ranging from 1 to 300 MPa. Once equilibrium is reached at the given suction, the samples are weighed and their volume is measured. A synthesis of the drying–wetting paths is given on Fig. 5. The swelling potential of the soil is evaluated using both indirect (or empirical methods Tables 2 and 3) and direct methods. Swell percentage and swell pressure of the soil are measured in a conventional oedometer apparatus according to ASTM (D 4546-85). The test specimens are 70 mm in diameter and the height varies between 12 and 24 mm. The swell percentage is measured under a nominal pressure of 0.7, 2.0 and 6.3 kPa. Swelling pressure of the soil is measured by the conventional consolidation test method (free swell and load, ASTM D 4546-85 method A) and by a constant volume method (ASTM D 4546-85 method C). The test parameters and results for each specimen are given in Tables 4 and 5, and on Fig. 7. Cyclic swell–shrink tests are carried out on similar samples taken from the same monolith. A scheme that permits the study of the clayey soil behaviour at the extreme states of wetting and drying is chosen. The test begins by wetting the samples at their natural moisture content and density. When swelling is stabilized, the water is removed from around the samples and they are dried in an oven maintained at 45°C until the vertical deformation (shrinkage) is stabilised and are then rewetted and so on. Some experiments are stopped at different swelling phases for microstructural study of the soil. The test parameters of the specimens are given in Table 9 and the results are shown in Figs. 9 and 10. The evolution of the microstructure during wetting and drying cycles is investigated using scanning electron microscope and mercury intrusion porosimetry. Observations are made only on soil specimens taken at the end of the swelling phase of the selected cycles. In order to preserve the microstructure, the specimens are cut in small pieces, frozen by liquid nitrogen and finally sublimated. The results of the drying–wetting path including the water retention curve are shown on Fig. 5. The results show that on the drying path (in the void ratio versus water content plane) the soil first follows nearly the saturation line and then, as the water content decreases, the void ratio tends towards a constant value. A shrinkage limit of w = 14.5 % and a corresponding suction value of 15 MPa is deduced from this path. An air entry value of 10 MPa is obtained from degree of saturation versus suction curve. The wetting path shows that the wetting–drying path is reversible for suction values higher than 60 MPa. The different indirect methods used to assess the swelling potential of the Argile verte de Romainville show a general agreement with respect to its swelling potential ranging from high to very high (Table 3). Examination of the free swell test results shows that the Argile verte de Romainville exhibits swell percentage in the range of 15–26% and that its degree of swelling depends on the initial conditions (water content, dry density) and the applied load (Table 4). The higher the water content and the applied load, the lower the swell percentage. A specimen taken parallel to the bedding plane shows similar values of swell percentage with a steep volume change versus time curve indicating an anisotropy of permeability. The two direct methods used to assess the swelling pressure of the Argile verte de Romainville give different values (Table 5). The values obtained by the constant volume method are relatively close and are about 700 kPa. Lower values varying between 360 and 540 kPa are obtained by the conventional consolidation test (free swell-consolidation). This indicates that besides the initial conditions, the swelling pressure is strongly dependent on the stress path followed. The results obtained from the wetting–drying cycle tests show that the magnitude of the first swell cycle is controlled by the initial water content, the maximum deformation occurring on the second cycle and the stabilization of swelling deformation from the third cycle (Figs. 9, 10). Furthermore, the experimental data indicate that upon repeated wetting and drying, the swelling rate of the soil becomes faster, which is explained by an increase in permeability of the soil due to the development of preferential flow paths (micro cracks) on drying. With an increasing number of cycles, a permanent increase in the volume of the samples is observed. This suggests that the swelling–shrinkage behaviour of expansive soils is not completely reversible. Mercury intrusion porosimetry analysis and SEM observations before and after different numbers of cyclic swelling indicate that the swelling–shrinkage cycles are accompanied by a continual reconstruction of the soil structure (Figs. 11, 12). The mercury intrusion porosimetry results show that with an increasing number of wetting–drying cycles the pore volume and the average diameter of the pores increase progressively (Fig. 11). Larger modifications are observed in the pores with radius in the range of 0.1–5 μm. SEM observations also show further destruction of large aggregates and disorientation of structural elements as the number of cycles increases (Fig. 12). After the fifth cycle, the soil original structure is totally lost and a disoriented homogeneous and loose structure with more homogeneous pore spaces is observed (Fig. 12d).