One-pot hydrothermal synthesis of uniformly cubic Co3O4 nanocrystals

Cubic Co₃O₄ nanocrystals (NCs) were synthesized by a one-pot hydrothermal reaction in the presence of the oxidant KClO₃ and the capping reagent polyvinylpyrrolidone (PVP). The as-prepared Co₃O₄ NCs were uniformly cubic with sharp edges and good crystallinity and showed flat surfaces with {100} facets. The capped PVP could be removed without losing cubic shape by heating the NCs at 300 °C.     

Structure Analysis of Molten Ba–Ge Alloys Using Electrostatic Levitation Technique Combined With High-Energy X-Ray Diffraction

High-energy synchrotron X-ray diffraction experiments were performed for clathrate-forming barium–germanium (Ba–Ge) alloys in the liquid state near the eutectic composition. The accurate structure information of highly reactive melts has been obtained by applying electrostatic levitation technique as a containerless method. The structure information obtained from the reverse Monte Carlo simulation suggests that the short-range ordering between the Ge atoms occurs with the addition of Ba atoms in the liquid Ba–Ge alloys. This can be associated with the formation of the cage-like structure composed of the Ge atoms in the liquid alloys near the eutectic composition.     

Unexpected discoveries and S-invention of design requirements: important vehicles for a design process

Designers, during a conceptual design process, do not just synthesize solutions that satisfy initially given requirements, but also invent design issues or requirements that capture important aspects of the given problem. How do they do this? What becomes the impetus for the invention of important issues or requirements? So-called ‘unexpected discoveries', the acts of attending to visuo-spatial features in sketches which were not intended when they were drawn, are believed to contribute to it. The purpose of the present research is to verify this hypothesis. Analysing the cognitive processes of a practising architect in a design session, we found that in about a half of his entire design process there were bi-directional relations between unexpected discoveries and the invention of issues or requirements. Not only did unexpected discoveries become the driving force for the invention of issues or requirements, but also the occurrence of invention, in turn, tended to cause new unexpected discoveries. This has provided empirical evidence for two anecdotal views of designing. First, designing is a situated act; designers invent design issues or requirements in a way situated in the environment in which they design. We call inventions of this sort situated-invention (S-invention). Secondly, a design process progresses in such a way that the problem-space and the solution-space co-evolve. Further, this has brought a pedagogical implication as well as an insight about an important aspect of learning by experience in design.     

Parallel processing of network calculations in order to speed up transient stability analysis

Power systems have become very large, and in addition the forthcoming open electricity market will make power system operation more complex. Therefore, power system operation must become more efficient and flexible. Very fast power system simulation is a means of achieving more sophisticated power system on‐line monitoring and control, and parallel computing is a key technology for very fast power system simulation. This paper proposes an efficient and fast parallel network calculation algorithm that will contribute to the development of fast power system simulation programs. Numerical examples show that the proposed method using six processors is about 4.0 times as fast as the usual serial algorithm when applied to a large‐scale radial power system and about 3.2 times as fast when applied to a large‐scale loop power system. © 2001 Scripta Technica, Electr Eng Jpn, 135(3): 26–36, 2001     

Conditional transformation of immunoglobulin mutation pattern from gene conversion into point mutation by controlling XRCC3 expression in the DT40 B cell line

A hypermutating B cell line DT40 is useful for screening antibodies and improving affinity of the selected antibodies in vitro. To perform affinity maturation efficiently, we generated an engineered DT40 line whose immunoglobulin mutation pattern can be transformed from gene conversion into point mutation by conditional suppression of XRCC3 expression.     

Structure of effective catalyst layers around bubbles in a fluidized catalyst bed

In a fluidized catalyst bed, the reactant gas transfers from the bubble phase to the emulsion phase and reactions proceed in the emulsion phase. The catalyst particles around the bubbles should contact the gases containing a high concentration of the reactants. Therefore, the effect of the catalysts around the bubbles is very important for estimating the conversion and selectivity in the reactor. In order to study the role of these catalysts, the hydrogenation of carbon dioxide was carried out in a fluidized catalyst bed. Based on the results, the amount of the catalyst that was effective for the reaction was calculated. In addition, the shape of the bubbles ascending in the fluidized catalyst bed was observed using a fast X-ray computer tomography (CT) scanner. The structure of the bubbles in the fluidized catalyst bed was very complicated and the surface area of the bubbles was much greater than the obtained when assuming spherical shaped bubble. By assuming that effective catalysts existed around the bubbles, the thickness of catalyst layer was obtained. Finally, the 3-dimensional images of the catalyst layers around the bubbles were reconstructed.     

Osteogenic activity of human periosteal sheets cultured on salmon collagen-coated ePTFE meshes

Our animal implantation studies have demonstrated that, after osteogenic processing, cultured human periosteal sheets form osteoid tissue ectopically without the aid of conventional scaffolding materials. To improve the osteogenic activity of these periosteal sheets, we have tested the effects of including a scaffold made of salmon collagen-coated ePTFE mesh. Periosteal sheets were produced with minimal manipulation without enzymatic digestion. Outgrown cells penetrated into the coated mesh fiber networks to form complex multicellular layers and increased expression of alkaline phosphatase activity in response to the osteoinduction. In vitro mineralization was notably enhanced in the original tissue segment regions, but numerous micro-mineral deposits were also formed on the coated-fiber networks. When implanted subcutaneously into nude mice, periosteal sheets efficiently form osteoid around the mineral deposits. These findings suggest that the intricate three-dimensional mesh composed of collagen-coated fibers substantially augmented the osteogenic activity of human periosteal sheets both in vitro and in vivo.     

Physiological role of Ca2+-permeable nonselective cation channel in endothelin-1-induced contraction of rabbit aorta

The alpha-amylase of Streptomyces sp. IMD 2679 was subject to catabolite repression. Four different growth rates were achieved when the organism was grown at 40 degrees C and 55 degrees C in the presence and absence of cobalt, with an inverse relationship between alpha-amylase production and growth rate. Highest alpha-amylase yields (520 units/ml) were obtained at the lowest growth rate (0.062 h-1), at 40 degrees C in the absence of cobalt, while at the highest growth rate (0.35 h-1), at 55 degrees C in the presence of cobalt, alpha-amylase production was decreased to 150 units/ml. As growth rate increased, the rate of specific utilisation of the carbon source maltose also increased, from 46 to 123 micrograms maltose (mg biomass)-1 h-1. The pattern and levels of alpha-glucosidase (the enzyme degrading maltose) detected intracellularly in each case, indicate that growth rate effectively controls the rate of feeding of glucose to the cell, and thus catabolite repression.