全文获取类型
收费全文 | 3067篇 |
免费 | 36篇 |
国内免费 | 13篇 |
专业分类
电工技术 | 102篇 |
综合类 | 4篇 |
化学工业 | 662篇 |
金属工艺 | 100篇 |
机械仪表 | 85篇 |
建筑科学 | 37篇 |
能源动力 | 169篇 |
轻工业 | 153篇 |
水利工程 | 11篇 |
石油天然气 | 6篇 |
无线电 | 368篇 |
一般工业技术 | 515篇 |
冶金工业 | 668篇 |
原子能技术 | 52篇 |
自动化技术 | 184篇 |
出版年
2023年 | 20篇 |
2022年 | 26篇 |
2021年 | 50篇 |
2020年 | 20篇 |
2019年 | 24篇 |
2018年 | 24篇 |
2017年 | 28篇 |
2016年 | 41篇 |
2015年 | 35篇 |
2014年 | 63篇 |
2013年 | 136篇 |
2012年 | 90篇 |
2011年 | 131篇 |
2010年 | 109篇 |
2009年 | 117篇 |
2008年 | 122篇 |
2007年 | 116篇 |
2006年 | 87篇 |
2005年 | 77篇 |
2004年 | 76篇 |
2003年 | 119篇 |
2002年 | 83篇 |
2001年 | 72篇 |
2000年 | 54篇 |
1999年 | 87篇 |
1998年 | 317篇 |
1997年 | 174篇 |
1996年 | 115篇 |
1995年 | 73篇 |
1994年 | 82篇 |
1993年 | 72篇 |
1992年 | 39篇 |
1991年 | 35篇 |
1990年 | 45篇 |
1989年 | 36篇 |
1988年 | 25篇 |
1987年 | 28篇 |
1986年 | 26篇 |
1985年 | 31篇 |
1984年 | 26篇 |
1983年 | 31篇 |
1982年 | 16篇 |
1981年 | 14篇 |
1980年 | 23篇 |
1979年 | 13篇 |
1978年 | 11篇 |
1977年 | 12篇 |
1976年 | 21篇 |
1975年 | 11篇 |
1974年 | 10篇 |
排序方式: 共有3116条查询结果,搜索用时 15 毫秒
71.
Deokho Lee Yohei Tomita Yukihiro Miwa Heonuk Jeong Ari Shinojima Norimitsu Ban Shintaro Yamaguchi Ken Nishioka Kazuno Negishi Jun Yoshino Toshihide Kurihara 《International journal of molecular sciences》2022,23(23)
Cardiovascular abnormality-mediated retinal ischemia causes severe visual impairment. Retinal ischemia is involved in enormous pathological processes including oxidative stress, reactive gliosis, and retinal functional deficits. Thus, maintaining retinal function by modulating those pathological processes may prevent or protect against vision loss. Over the decades, nicotinamide mononucleotide (NMN), a crucial nicotinamide adenine dinucleotide (NAD+) intermediate, has been nominated as a promising therapeutic target in retinal diseases. Nonetheless, a protective effect of NMN has not been examined in cardiovascular diseases-induced retinal ischemia. In our study, we aimed to investigate its promising effect of NMN in the ischemic retina of a murine model of carotid artery occlusion. After surgical unilateral common carotid artery occlusion (UCCAO) in adult male C57BL/6 mice, NMN (500 mg/kg/day) was intraperitoneally injected to mice every day until the end of experiments. Electroretinography and biomolecular assays were utilized to measure ocular functional and further molecular alterations in the retina. We found that UCCAO-induced retinal dysfunction was suppressed, pathological gliosis was reduced, retinal NAD+ levels were preserved, and the expression of an antioxidant molecule (nuclear factor erythroid-2-related factor 2; Nrf2) was upregulated by consecutive administration of NMN. Our present outcomes first suggest a promising NMN therapy for the suppression of cardiovascular diseases-mediated retinal ischemic dysfunction. 相似文献
72.
Long Preservation of AAV-Transduced Fluorescence by a Modified Organic Solvent-Based Clearing Method
Tao Lu Munehisa Shinozaki Narihito Nagoshi Masaya Nakamura Hideyuki Okano 《International journal of molecular sciences》2022,23(17)
The development of tissue clearing technologies allows 3D imaging of whole tissues and organs, especially in studies of the central nervous system innervated throughout the body. Although the three-dimensional imaging of solvent-cleared organs (3DISCO) method provides a powerful clearing capacity and high transparency, the rapid quenching of endogenous fluorescence and peroxide removal process decreases its practicability. This study provides a modified method named tDISCO to solve these limitations. The tDISCO protocol can preserve AAV-transduced endogenous EGFP fluorescence for months and achieve high transparency in a fast and simple clearing process. In addition to the brain, tDISCO was applied to other organs and even hard bone tissue. tDISCO also enabled us to visualize the long projection neurons and axons with high resolution. This method provides a fast and simple clearing protocol for 3D visualization of the AAV- transduced long projection neurons throughout the brain and spinal cord. 相似文献
73.
Minami Yamauchi Toshihiro Sato Ayana Otake Masaki Kumondai Yu Sato Masafumi Kikuchi Masamitsu Maekawa Hiroaki Yamaguchi Takaaki Abe Nariyasu Mano 《International journal of molecular sciences》2022,23(15)
Patients with liver diseases not only experience the adverse effects of liver-metabolized drugs, but also the unexpected adverse effects of renally excreted drugs. Bile acids alter the expression of renal drug transporters, however, the direct effects of bile acids on drug transport remain unknown. Renal drug transporter organic anion-transporting polypeptide 4C1 (OATP4C1) was reported to be inhibited by chenodeoxycholic acid. Therefore, we predicted that the inhibition of OATP4C1-mediated transport by bile acids might be a potential mechanism for the altered pharmacokinetics of renally excreted drugs. We screened 45 types of bile acids and calculated the IC50, Ki values, and bile acid–drug interaction (BDI) indices of bile acids whose inhibitory effect on OATP4C1 was >50%. From the screening results, lithocholic acid (LCA), glycine-conjugated lithocholic acid (GLCA), and taurine-conjugated lithocholic acid (TLCA) were newly identified as inhibitors of OATP4C1. Since the BDI index of LCA was 0.278, LCA is likely to inhibit OATP4C1-mediated transport in clinical settings. Our findings suggest that dose adjustment of renally excreted drugs may be required in patients with renal failure as well as in patients with hepatic failure. We believe that our findings provide essential information for drug development and safe drug treatment in clinics. 相似文献
74.
75.
Rapid and simultaneous enumeration of viable Enterobacteriaceae and viable Pseudomonas spp. in milk was achieved by using multicolor fluorescence in situ hybridization (FISH) with oligonucleotide probes based on 16S ribosomal RNA (rRNA) sequences in combination with a microcolony growth method (multicolor microcolony-FISH; MMC-FISH). The procedure of MMC-FISH method is rather simple; that is milk clearing, filtration of cells, incubation, hybridization and enumeration. Enumeration of targeted bacteria in logarithmic growth phase, stationary phase, or in a starved state in milk by MMC-FISH required 5-7 h, while it took 1-3 days to test for Escherichia coli and Pseudomonas putida by the conventional culture method. The numbers of E. coli and P. putida in each phase or in a starved state in milk determined by MMC-FISH were almost the same or greater than the number of colony forming units determined by the plate counting method. The MMC-FISH allows rapid examination of contamination in milk by viable Enterobacteriaceae and Pseudomonas spp. with growth potential. 相似文献
76.
Shiomi N Yamaguchi Y Nakai H Fujita T Katsuda T Katoh S 《Journal of Bioscience and Bioengineering》2006,102(3):206-209
The rates of degradation of cyanuric acid, a key intermediate in a metabolic pathway of s-triazine herbicides, were measured for Pseudomonas sp. NRRL B-12227. The rate of degradation was affected by the rate of cyanuric acid transport through cell membranes and the activity of cyanuric acid amidohydrolase inside the cells. At low concentrations of cyanuric acid, the acclimation of cells to cyanuric acid and/or added nutrients effectively enhanced the degradation rate. The strain was also applied to bioremediation using a Bioremediation with Self-Immobilization System (BSIS), in which Pseudomonas sp. NRRL B-12227 cells were co-immobilized with Bacillus subtilis, the latter of which secretes a viscous polymer, in a shallow layer of soil packed in a column. More than 70% of the Pseudomonas sp. NRRL B-12227 cells were co-immobilized with the B. subtilis in a 7.5 cm layer of the packed soil by self-aggregation. More than 60% of the 1 mM cyanuric acid supplied to the packed soil was degraded in this layer during a 72 h period. 相似文献
77.
Awazu K Nomura C Yamaguchi M Obana H 《Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan》2011,52(3):199-204
A simple and practical method was developed for the determination of histamine in fish and fish products by solid-phase extraction and fluorescence derivatization. Histamine was extracted with trichloroacetic acid. The extract was neutralized and diluted with phosphate buffer (pH 6.8), and cleaned up with a tandem-connected octadecyl silica (ODS) and strong cation exchange silica (SCX) cartridge. After removal of the solvent, histamine was derivatized with fluorescamine and analyzed by ion-paired reversed-phase high-performance liquid chromatography with fluorescence detection. Recovery tests of histamine from six kinds of fish and fish products showed acceptable recovery (83-92%) with low relative standard deviation (less than 5%). This method could be useful for determination of histamine in fish. 相似文献
78.
Molecular characterization of antibiotic-resistant salmonella isolates from retail meat from markets in northern Vietnam 总被引:1,自引:0,他引:1
A total of 118 Salmonella isolates were detected from 283 retail meat samples (135 pork and 148 chicken meat) purchased at retail markets in Northern Vietnam. Thirteen serovars, including Infantis, Anatum, Rissen, Reading, Emek, Typhimurium, Blockley, London, Newport, Derby, Weltevreden, Albany, and Hadar, were determined. Resistance to tetracycline (54.2%), sulfonamides (52.5%), streptomycin (41.5%), trimethoprim (36.4%), chloramphenicol (35.6%), and ampicillin (33.1%) was commonly seen in the Salmonella isolates. Fourteen [bla(TEM), bla(OXA-1), bla(PSE-1), aadA1, sul1, tetA, tetB, tetG, cmlA1, floR, dfrA1, dfrA12, aac(3)-IV, and aphA1-1AB ] of 17 resistance genes were detected from the isolates demonstrating resistance. Genes for plasmid-mediated quinolone resistance, such as qnrA, qnrB, qnrS, qepA, and acc(6')-1b-cr, were not detected in 23 quinoloneresistant isolates. The substitution TCC to TTC at codon 83 of gyrA was found in the 18 quinolone-resistant isolates. The data revealed that resistant Salmonella strains were widely distributed in Northern Vietnam via the food chain and that they might contain multiple genes specifying identical resistance phenotypes. Thus, further studies are necessary to clarify the mechanisms of antibiotic resistance in Salmonella strains and their spread in the livestock market. 相似文献
79.
Samsur M Takatani T Yamaguchi Y Sagara T Noguchi T Arakawa O 《Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan》2007,48(1):13-18
The paralytic shellfish poison (PSP)-producing dinoflagellate Gymnodinium catenatum (Gc) was fed to the short-necked clam Tapes japonica, and the accumulation, transformation and elimination profiles of PSP were investigated by means of high-performance liquid chromatography with postcolumn fluorescence derivatization (HPLC-FLD). The short-necked clams ingested most of the Gc cells (4 x 10(6) cells) supplied as a bolus at the beginning of the experiment, and accumulated a maximal amount of toxin (181 nmol/10 clams) after 12 hr. The rate of toxin accumulation at that time was 16%, which rapidly decreased thereafter. During the rearing period, a variation in toxin composition, derived presumably from the transformation of toxin analogues in the clams, was observed, including a reversal of the ratio of C2 to C1, and the appearance of carbamate (gonyautoxin (GTX) 2, 3) and decarbamoyl (dc) derivatives (decarbamoylsaxitoxin (dcSTX) and dcGTX2, 3), which were undetectable in Gc cells. The total amount of toxin contained in clams and residue (remaining Gc cells and/or excrement in the rearing tank) gradually declined, and only about 1% of the supplied toxin was detected at the end of the experiment. 相似文献
80.
Shibata H Takahashi M Yamaguchi I Kobayashi S 《Journal of Bioscience and Bioengineering》1999,88(3):244-249
Sulfides (S2),SH-) such as hydrogen sulfide belong to a class of sulfur compounds with unpleasant odors. In order to confer sulfide-oxidizing ability on the intestine-inhabiting bacteria, the sulfide-quinone oxidoreductase gene (sqr) in Rhodobacter capsulatus DSM-155 and genes for quinone biosynthesis (ubiC, ubiA and ispB) in Escherichia coli XL1 Blue-MRF' were transduced into E. coli BL21(DE3). Plasmids pT7-7 and pSTV were used as vectors of sqr, and ubiCA and ispB, respectively. The recombinants sqr-BL21(DE3) and ubiCA,ispB-sqr-BL21(DE3) were successfully constructed. The maximal sulfide-removing activities of the whole cells and membrane fractions of sqr-BL21(DE3) attained at pH 8.0 and 7.8, were 267 nmol/mg cells (dry weight)/min and 1250 nmol/mg membrane fraction (protein)/min, respectively. The molecular ratio of sulfide (S2-) oxidized and oxygen (O2) consumed was 2:1. SQR activity in the recombinant cells was positively restricted under anaerobic conditions and also by the addition of electron transfer inhibitors. Ubiquinone-8 (UQ-8) biosynthesis in the cells of ubiCA,ispB-sqr-BL21(DE3) increased as much as 2.2-fold compared with that of (pSTV)-sqr-BL21(DE3) during the 12-16 h incubation period. The maximal sulfide removal in the quinone-raised E. coli was attained slightly earlier, however, SQR activities thereafter were lower than those in (pSTV)-sqr-BL21(DE3). 相似文献