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101.
J Nesvera M Pátek J Hochmannová Z Abrhámová V Becvárová M Jelínkova J Vohradsky 《Canadian Metallurgical Quarterly》1997,179(5):1525-1532
The complete nucleotide sequence (4,826 bp) of the cryptic plasmid pGA1 from Corynebacterium glutamicum was determined. DNA sequence analysis revealed four putative coding regions (open reading frame A [ORFA], ORFA2, ORFB, and ORFC). ORFC was identified as a rep gene coding for an initiator of plasmid replication (Rep) according to the high level of homology of its deduced amino acid sequence with the Rep proteins of plasmids pSR1 (from C. glutamicum) and pNG2 (from Corynebacterium diphtheriae). This function was confirmed by deletion mapping of the minimal replicon of pGA1 (1.7 kb) which contains only ORFC. Deletion derivatives of pGA1 devoid of ORFA exhibited significant decreases in the copy number in C. glutamicum cells and displayed segregational instability. Introduction of ORFA in trans into the cells harboring these deletion plasmids dramatically increased their copy number and segregational stability. The ORFA gene product thus positively influences plasmid copy number. This is the first report on such activity associated with a nonintegrating bacterial plasmid. The related plasmids pGA1, pSR1, and pNG2 lacking significant homology with any other plasmid seem to be representatives of a new group of plasmids replicating in the rolling-circle mode. 相似文献
102.
Jan van Eijck 《Formal Aspects of Computing》1994,6(1):766-787
Presuppositions of utterances are the pieces of information you convey with an utterance no matter whether your utterance is true or not. We first study presupposition in a very simple framework of updating propositional information, with examples of how presuppositions of complex propositional updates can be calculated. Next we move on to presuppositions and quantification, in the context of a dynamic version of predicate logic, suitably modified to allow for presupposition failure. In both the propositional and the quantificational case, presupposition failure can be viewed as error abortion of procedures. Thus, a dynamic assertion logic which describes the preconditions for error abortion is the suitable tool for analysing presupposition. 相似文献
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107.
R Herruzo-Cabrera L Malo-González ME Calle Purón MJ Vizcaíno-Alcaide J Del Rey-Calero 《Canadian Metallurgical Quarterly》1993,9(4):442-446
A cross-sectional study was performed to obtain risk factors for hepatitis B disease, HBsAg carriers and immunised personnel, among 2470 workers in a general hospital in Madrid, Spain. The data obtained were analyzed with multiple logistic regression to obtain beta coefficients for variables. The results of the analysis show that being a nurse or being regularly exposed to blood are the most important risk factors for hepatitis B acquisition. The length of time working at the same job activity was also a risk factor. The resulting beta coefficients allow the construction for a hepatitis non-immunised, HBsAg carrier and immunised HBV status, which can select subjects for a hepatitis B vaccination program. 相似文献
108.
María P. Albano Liliana B. Garrido 《Journal of Materials Synthesis and Processing》2002,10(4):211-218
Si3N4powders coated with 6 wt% Y2O3and 4 wt% Al2O3were prepared by coprecipitation. The resulting powders were dispersed in water at different pH values and with addition of various amounts of ammonium polyacrylate (NH4PA) to produce 32 vol% slips. The influence of the amount of NH4PA solution added and pH on the rheological properties of 32 vol% coated Si3N4slips were studied. In addition, the sintered density of cast samples was determined and related to the degree of slip dispersion. The adsorption of the NH4PA on the coated particle surface was rather high and the surface became saturated near 0.86 mg/m2at pH 9.2. High NH4PA concentrations (1.7–3 wt%) were necessary to obtain well dispersed 32 vol% coated Si3N4slips at pH 9.2. The best stabilization was obtained with the addition of 2.3 wt% NH4PA; in this condition, the viscosity reached a minimum value of 35 mPa.s at 100 s–1. The slip viscosity increased with increasing pH from 9.2 to 10.2. Slips with low viscosities gave a more dense packing of cast samples and consequently higher sintered density values. 相似文献
109.
Certain new sulfide-smelting processes and coal-gasification processes generate high-strength sulfur dioxide streams, for
which a new process for converting sulfur dioxide to elemental sulfur needs to be developed because no process exists that
is generally and economically applicable to the treatment of such streams. A thermodynamic and experimental investigation
to develop a new process for converting sulfur dioxide to elemental sulfur by a cyclic process involving calcium sulfide and
calcium sulfate without generating secondary pollutants was carried out. In this process, the starting raw material, calcium
sulfate, is reduced by a suitable reducing agent, such as hydrogen, to produce calcium sulfide, which is used to reduce sulfur
dioxide to elemental sulfur vapor and calcium sulfate. The latter is, in turn, reduced to regenerate calcium sulfide. In this
Part I, detailed experimental results are presented on the kinetics of the reaction between sulfur dioxide and calcium-sulfide
pellets, which produces elemental sulfur and calcium sulfate. The experiments were carried out at temperatures between 1023
and 1088 K and sulfur-dioxide partial pressures between 9 and 60 kPa by the use of a thermogravimetric analysis (TGA) technique.
The rate of this reaction was demonstrated by the conversion of 40 pct calcium-sulfide pellets obtained from the hydrogen
reduction of fresh calcium sulfate in 10 minutes at 1073 K under a sulfur-dioxide partial pressure of 43 kPa. The reactivity
decreased somewhat during the first three cycles but remained largely unchanged thereafter up to the tenth cycle. This characteristic
of the pellets is important because the solids must be reusable for repeated cycles to avoid generating secondary pollutants.
A pore-blocking model was found to fit the reaction rate. The reaction is first order with respect to sulfur-dioxide partial
pressure and has an activation energy of 101 to 134 kJ/mol (24 to 32 kcal/mol) for calcium-sulfide pellets reacted and regenerated
several different times. Sulfur dioxide-containing streams from certain sources, such as the regenerator off-gas from an integrated-gasification,
combined-cycle, desulfurization unit and new sulfide-smelting plants, contain much higher partial pressures of SO2. In these cases, the rate of the first reaction is expected to be proportionally higher than in the test conditions reported
in this article. The reduction kinetics of calcium-sulfate pellets with hydrogen gas is reported in the accompanying Part
II. 相似文献
110.
Microbiological denitrification of red beet juice 总被引:1,自引:0,他引:1
Dorota Walkowiak-Tomczak 《European Food Research and Technology》2002,215(5):401-406
The purpose of study was the determination of optimal conditions for red beet juice denitrification by Paracoccus denitrificans bacteria as well as the evaluation of the usefulness of microbiologically treated juice for the production of a natural colouring preparation for foods. Total reduction of nitrates was found during bacterial culturing at 25 and 30 °C, at an initial pH of 7.0-8.0 and juice osmotic pressure of 900-1100 mOsm/kg. Microbiological denitrification procedure affected colour, taste and aroma of the juice. However, the use of juice as food colouring and component was made feasible by acidification and partial evaporation of water together with volatile substances. 相似文献