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21.
P Franco O Massa M Garcia-Rocha F Chiaradonna C Iaccarino I Correas E Mendez J Avila F Blasi MP Stoppelli 《Canadian Metallurgical Quarterly》1998,273(42):27734-27740
We recently reported that in vivo phosphorylation of urokinase-type plasminogen activator on Ser138/303 prevents its catalytic-independent ability to promote myelomonocytic cell adherence and motility. We now show that Ca2+ activated, phospholipid-dependent protein kinase C from rat brain phosphorylates in vitro a peptide corresponding to prourokinase residues 133-143 (DGKKPSSPPEE) and the full-length molecule on Ser138/139. The in vivo involvement of the protein kinase C isoenzyme family is supported by the finding that inhibition of kinase C activity prevents prourokinase phosphorylation on Ser138/303 in A431 human carcinoma cells. Conversely, a short treatment of A431 cells with phorbol myristate acetate increases the extent of phosphorylated prourokinase and, concomitantly, affects its function; under these conditions, the capability of prourokinase to up-regulate U937 monocyte-like cell adherence is severely impaired, although receptor binding is unaltered. By the aid of a "phosphorylation-like" variant (Ser138 to Glu) we show that modification of Ser138 is sufficient to confer to prourokinase the antagonistic properties observed following in vivo stimulation of protein kinase C activity. These observations provide the first evidence that protein kinase C directs the formation of a receptor competitive antagonist by regulating the in vivo phosphorylation state of prourokinase. 相似文献
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We show how to support efficient back traversal in a unidirectional list, using small memory and with essentially no slowdown in forward steps. Using O(lgn) memory for a list of size n, the i’th back-step from the farthest point reached so far takes O(lgi) time in the worst case, while the overhead per forward step is at most ? for arbitrary small constant ?>0. An arbitrary sequence of forward and back steps is allowed. A full trade-off between memory usage and time per back-step is presented: k vs. kn1/k and vice versa. Our algorithms are based on a novel pebbling technique which moves pebbles on a virtual binary, or n1/k-ary, tree that can only be traversed in a pre-order fashion. 相似文献
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Del Villar I. Matias I.R. Arregui F.J. Claus R.O. 《Nanotechnology, IEEE Transactions on》2005,4(2):187-193
A fiber-optic sensor sensitive to hydrogen peroxide has been designed based on the electrostatic layer-by-layer self-assembly method. Prussian blue has been deposited in a polymeric structure formed by Poly(allylamine hydrochloride) and poly(acrylic acid). The concentration that can be detected range between 10/sup -6/-10/sup -3/ M, and recovery of the sensor after immersion into a reductive agent was demonstrated. The response of the sensor is independent of the pH for values that range between 4-7.4. Some rules for estimation of the refractive index of the material deposited and the thickness of the bilayers are also presented. 相似文献
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Matias Urbieta Ana Oliveira João Araújo Armanda Rodrigues Ana Moreira Sílvia Gordillo Gustavo Rossi 《Innovations in Systems and Software Engineering》2014,10(1):59-75
Spatial concerns of Web geographical information systems (Web-GIS) are inherently crosscutting and volatile: crosscutting because they affect multiple functionalities of Web-GIS systems, and volatile because their status may change often. If these concerns are not modularized properly, the quality of Web-GIS services, particularly with regard to adaptation and evolution, can be severely compromised. This paper uses aspect-orientation to model crosscutting and volatile spatial concerns. By modeling both types of concerns, crosscutting and volatile, as candidate aspects, one can use dynamic weaving to add or remove them from a system at runtime. The aspect-oriented approach proposed starts with the identification and specification of crosscutting concerns and follows by composing these using modeling aspects using a transformation approach, an aspect-oriented modeling technique. The conflicts that can emerge due to the composition order are also taken into consideration. Finally, this paper proposes a set of reusable GIS crosscutting concerns, documenting them in a concern catalogue. 相似文献
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Recent work on reactive flash sintering of powders of two oxides, bismuth and of iron oxide, into pure single phase bismuth ferrite, which was accomplished in a few seconds at low furnace temperatures, is expanded to four constituents, alumina, lithia, zirconia, and lanthana, to produce reasonably dense polycrystals of a predominantly single phase, cubic LLZO(Al). Transformation and sintering occur concurrently at a furnace temperature near 700°C, in ambient atmosphere, in just a few seconds. The process may simplify the preparation of complex ceramics with new chemistries and dopants, which are predicted from ab intio calculations to have special attributes, not only because the powders sinter quickly at low temperatures, but also because the need for stoichiometric powders as starting materials is obviated. 相似文献
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We report the results of an empirical study demonstrating the value of using physiological compliance as a measure of social presence during digital game playing. The physiological activity (facial EMG, electrodermal activity, cardiac activity and respiration) of 21 dyads were acquired synchronously while they were playing a digital game either cooperatively or competitively and either at home or in the laboratory. Physiological compliance was defined as the correlation between the physiological signals of the dyad members. The results of this study confirm that physiological compliance is higher in a conflicting situation than when playing cooperatively. Importantly, the results also demonstrate that physiological compliance is related to self-reported social presence. This suggests that physiological compliance is not limited to negative situations but rather increases due to rich interactions. Only minor differences in physiological compliance were observed between home play and laboratory play, suggesting the ecological validity of laboratory measures. Finally, we propose that compliance measures can be considered as objective indices of social presence in digital gaming. 相似文献
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Metal‐Organic Framework Surface Functionalization: GraftFast Surface Engineering to Improve MOF Nanoparticles Furtiveness (Small 40/2018)
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