In vitro degradation of dermal sheep collagen cross-linked using a water-soluble carbodiimide

Bacterial collagenase was used to study the susceptibility of dermal sheep collagen (DSC) cross-linked with a mixture of the water-soluble carbodiimide 1-ethyl-3-(3-dimethyl aminopropyl)-carbodiimide hydrochloride and N-hydroxysuccinimide (E/N-DSC) towards enzymatic degradation. Contrary to non-cross-linked DSC (N-DSC), which had a rate of weight-loss of 18.1% per hour upon degradation, no weight loss was observed for E/N-DSC during a 24 h degradation period. The tensile strength of the E/N-DSC samples decreased during this time period, resulting in partially degraded samples having 80% of the initial tensile strength remaining. The susceptibility of E/N-DSC samples towards enzymatic degradation could be controlled by varying the degree of cross-linking of the samples. Ethylene oxide sterilization of E/N-DSC samples made the material more resistant against degradation compared with non-sterilized E/N-DSC samples. This may be explained by a decrease of the adsorption of bacterial collagenase onto the collagen owing to reaction of ethylene oxide with remaining free amine groups in the collagen matrix.     

Child health and the social environment of white and black children

Both poverty and other factors associated with race are related to child health. However, the mechanisms of these relationships have not been adequately specified. The purpose of this study was to explore the relationship of the social environment to child health status in black and white children and further, to explore whether the patterns of the effects of social class were different by race. This study provides further evidence that the social environment is strongly associated with child health status. Several risk factors are similar for both white and black children: mothers who view their own health as fair or poor are much more likely to rate their children in poor health. The presence of childhood chronic medical conditions is independently associated with poor health status regardless of race. However, the relative importance of several social risks for poor health status differs between white and black children. Specifically, while low family income is a consistent risk factor for poor health among white children, low income alone is not a risk factor for black children. Among black children, other social risks that are associated with poverty, such as low maternal education and a mother's perception of her own health as poor, increased the risk of poorer health in the child.     

Methods to assess physical activity with special reference tomotion sensors and accelerometers

Motion sensors may be applied for the assessment of physical activity. This paper reviews the evolution of these instruments from the mechanical pedometer to the electronic accelerometer. We conclude that for accurate assessment of physical activity under free living conditions the recently introduced accelerometer looks most promising, although little information was available regarding the reliability of these instruments. Subsequently, reliability of an accelerometer with a three-directional sensor was examined. Intrainstrument variation in a bench test was less than 8% during four measurements over a week. Interinstrument variation during treadmill experiments while subjects wore two accelerometers at the same time was on average 22% and was not improved after adjustment for differences found in the bench test. Reproducibility in the treadmill experiment was approximately 76, 85, and 95% at 3, 5, and 7 km/h, respectively. Bench testing revealed that the sensitivity of a piezoelectric element is prone to shifts, probably due to mechanical, electromagnetic, and/or temperature shock, which may be encountered during outdoor application. However, the relevance of the bench test in this study may be questioned, as results did not correspond with the findings in subjects. This needs further investigation.     

A high-frequency nonquasi-static analytical model including gate leakage effects for on-chip decoupling capacitors

This paper presents a compact model for on-chip decoupling capacitors (decaps) including gate-oxide leakage. The model makes use of only four parameters, namely, channel resistance, gate-oxide capacitance, and two parameters to quantify gate-oxide leakage, to predict the static and dynamic response of decaps. Quality indices have been defined to enable development of decap design guidelines and evaluation of performance of such capacitors. The model shows how the gate leakage and longer channel lengths severely affect the performance of on-chip decaps for both low and high frequencies. The model also shows that lumped models of decaps at high frequencies fail and have to be substituted by a distributed model. Application of the model uncovers tradeoffs for thin- and thick-oxide capacitors in an available 90-nm CMOS technology. For a general-purpose technology, a reference capacitance value has been realized using decaps with a discrete width and length. Our model predicts that thick-oxide n-channel (p-channel) capacitors require /spl sim/3.37x (/spl sim/3.31x) more silicon area and /spl sim/1.70x (/spl sim/1.17x) degraded time response as compared to their thin-oxide versions. The time response is even more degraded (/spl prop/L/sup 2/) when longer channel decaps are used. This paper contributes by defining performance benchmarks for decaps.     

Factors affecting contrasting results between self-reported and performance-based levels of physical limitation

Molecular electrostatic potential (MEP) maps of certain 3-methoxy flavone derivatives having different anti-picornavirus activities have been studied. Geometries of the molecules were optimised and charge distributions computed using the AM1 molecular orbital method. Hybridization displacement charges (HDC) were combined with the L?wdin charge distributions to compute the MEP maps. Reliability of the method of computing MEP maps was tested by studying certain other molecules for which ab initio MEP results are available. The anti-picornavirus activities of the flavones have been shown to be related with negative MEP values in two regions, one near the 3-methoxy group and another in a diagonally opposite region near the substituent attached to the C7 atom of the molecules.     

Activity of the plasma membrane H(+)-ATPase and optimal glycolytic flux are required for rapid adaptation and growth of Saccharomyces cerevisiae in the presence of the weak-acid preservative sorbic acid

The weak acid sorbic acid transiently inhibited the growth of Saccharomyces cerevisiae in media at low pH. During a lag period, the length of which depended on the severity of this weak-acid stress, yeast cells appeared to adapt to this stress, eventually recovering and growing normally. This adaptation to weak-acid stress was not due to metabolism and removal of the sorbic acid. A pma1-205 mutant, with about half the normal membrane H+-ATPase activity, was shown to be more sensitive to sorbic acid than its parent. Sorbic acid appeared to stimulate plasma membrane H+-ATPase activity in both PMA1 and pma1-205. Consistent with this, cellular ATP levels showed drastic reductions, the extent of which depended on the severity of weak-acid stress. The weak acid did not appear to affect the synthesis of ATP because CO2 production and O2 consumption were not affected significantly in PMA1 and pma1-205 cells. However, a glycolytic mutant, with about one-third the normal pyruvate kinase and phosphofructokinase activity and hence a reduced capacity to generate ATP, was more sensitive to sorbic acid than its isogenic parent. These data are consistent with the idea that adaptation by yeast cells to sorbic acid is dependent on (i) the restoration of internal pH via the export of protons by the membrane H+-ATPase in an energy-demanding process and (ii) the generation of sufficient ATP to drive this process and still allow growth.