High-throughput SNP genotyping by SBE/SBH

Despite much progress over the past decade, current single nucleotide polymorphism (SNP) genotyping technologies still offer an insufficient degree of multiplexing when required to handle user selected sets of SNPs. In this paper we propose a new genotyping assay architecture combining multiplexed solution-phase single-base extension (SBE) reactions with sequencing by hybridization (SBH) using universal DNA arrays such as all k-mer arrays. Simulation results on datasets both randomly generated and extracted from the NCBI dbSNP database suggest that the SBE/SBH architecture provides a flexible and cost-effective alternative to genotyping assays currently used in the industry, enabling genotyping of up to hundreds of thousands of SNPs per assay     

A neuro-evolutive technique applied for predicting the liquid crystalline property of some organic compounds

A simple self-adaptive version of the differential evolution algorithm was applied for simultaneous architectural and parametric optimization of feed-forward neural networks, used to classify the crystalline liquid property of a series of organic compounds. The developed optimization methodology was called self-adaptive differential evolution neural network (SADE-NN) and has the following characteristics: the base vector used is chosen as the best individual in the current population, two differential terms participate in the mutation process, the crossover type is binomial, a simple self-adaptive mechanism is employed to determine the near-optimal control parameters of the algorithm, and the integration of the neural network into the differential evolution algorithm is performed using a direct encoding scheme. It was found that a network with one hidden layer is able to make accurate predictions, indicating that the proposed methodology is efficient and, owing to its flexibility, it can be applied to a large range of problems.     

Poly(2-hydroxyethyl methacrylate-co-dodecyl methacrylate-co-acrylic acid): synthesis,physico-chemical characterisation and nafcillin carrier

In the present study polymeric microbeads of poly(2-hydroxyethyl methacrylate-co-dodecyl methacrylate-co-acrylic acid) or p(HEMA-co-dDMA-co-AA) were synthesised and characterized through FT-IR and scanning electron microscopy (SEM); their swelling behavior against saline solution was explored and their in vitro cytotoxicity was evaluated. Further, in order to elucidate kinetic aspects regarding the ternary system p(HEMA-co-dDMA-co-AA), a mathematical model of the reactivity ratios of the comonomers in the terpolymer has been conceived and analyzed. An intensified tendency of AA units accumulation in the copolymer has been noticed, in spite of HEMA units, while dDMA conserves in the copolymer the fraction from the feed. Three compositions have been selected for nafcillin-loading and their in vitro release capacity was evaluated. The compositions of 80:10:10 and 75:10:15 M ratios appear suitable for further in vivo testing, in order to be used as drug delivery systems in the treatment of different osseous diseases.     

On the Structure of Linear and Cyclic Codes over a Finite Chain Ring

We generalise structure theorems of Calderbank and Sloane for linear and cyclic codes over ℤ _pa to a finite chain ring. Our results are more detailed and do not use non-trivial results from Commutative Algebra. Received: January 25, 1999; revised version: November 17, 1999     

Fundamental solutions in micropolar elasticity

In this paper the general form of the fundamental solutions and fundamental matrices associated with the equations of the micropolar elastostatics as well as the integral representation by means of these matrices are determined. For the dynamic problem the time-periodic fundamental solutions and general fundamental solutions are determined. In this last case the explicit solution is given for $\text{ρ(γ + ?) = jμ}$.