Biomass production and small-scale testing of freeze-dried lactic acid bacteria starter strains for cassava fermentations

Based on their predominance in Gari fermentations, as well as suitable technological properties, Lactobacillus plantarum, Lactobacillus fermentum, Weissella paramesenteroides and Leuconostoc mesenteroides strains were investigated for their suitability for development as starter strains for this African traditional fermented cassava product. The strains were grown in optimized growth media in 2 L fermenters, harvested and freeze dried, and then tested in lab-scale cassava mash fermentation trials for their ability to ferment the cassava. The strains performed well and rapidly increased the titratable acidity from 1.1 to 1.3% at 24 h to 1.3–1.6% at 48 h. The benefit of including starter cultures was that it lowered the pH of the product much faster and to lower levels than in the uninoculated control fermentation. The results furthermore indicated that especially the L. plantarum-group strains could be produced as starter strains at low cost. Overall, the results of this study showed that starter strains could be easily and economically produced, and thus represent a feasible possibility for further development for application in the field.     

Sequential analysis of the effects of naltrexone on the environmental meditation of self-injurious behavior.

Accumulated evidence shows that biology and the environment can mediate self-injurious behavior (SIB) in persons with mental retardation. Whether pharmacological treatment alters the environmental mediation of self-injury is unclear. Opioid antagonist effects on sequential dependencies for self-injury were studied in the context of experimental single-subject double-blind placebo-controlled designs. Direct observational data were collected for 4 adult subjects in real time on daily rate of SIB and staff interactions. Clinically significant reductions (i.e., ≥ 33%) in SIB rate were observed for 3 of the 4 subjects. For all subjects, the magnitude of the sequential dependency between staff behavior and self-injury was significantly greater during treatment with naltrexone than during treatment with a placebo. Results are discussed in relation to behavioral mechanisms of action regulating medication effects for self-injury. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Haven or hell? Telework,flexibility and family in the e-society: a Marxist analysis

The reconfiguration of the home-work boundary that at-home telework entails has significant implications for gender issues and the use of ubiquitous information and communications technologies (ICTs). By presenting a Marx-inspired dialectical analysis of the family and home as both ‘haven and hell’, we develop a critique of proposed advantages for women home workers. Not only do we question the ability of ICTs to deliver the promises made on their behalf – we show how this socio-technical innovation may in fact contribute to compounding the double-burden of work associated with gender roles within the home. Contemporary critical understanding of the e-society should incorporate the influence of at-home teleworking because of its implications for the use of ubiquitous ICTs in the home environment, the shaping of work relations and its impact on gender issues. This increasing use of ICTs outside of the workplace is matched by the growing consensus within the European Union on the desirability of flexible working coupled with family friendly policies. This paper explores some of the rhetoric and research surrounding the proposed benefits of at home ‘telework’ and the likely cost–benefits, from an employee's perspective, in terms of increased freedom, reduced burden and ‘flexibility’.     

Inferential structure of the NEO Five-Factor Inventory: Construct validity of the Big Four personality clusters.

Four studies investigated the dimensionality of the NEO Five-Factor Inventory (NEO-FFI; P. T. Costa & R. R. McCrae, 1992). In Study 1, four inferential dimensions and four clusters represented the NEO-FFI when 114 undergraduates freely sorted items into categories. Construct validity for four item-clusters derived from the inferential space was obtained in Study 2 based on self-report with 304 undergraduates. Study 3 validated these inferential clusters using self and peer reports for 420 undergraduates. Study 4 validated the cluster scales for predicting quality of life and significant social and cultural behaviours for 110 undergraduates. Implications for implicit personality theory, the number of dimensions issue in personality, and test construction are discussed. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Frequency-stabilized mode-locked solid-state laser system forprecision range-Doppler imaging

We report measurements which show that an actively stabilized cw mode-locked Nd:YLF laser, in combination with a flashlamp-pumped Nd:glass amplifier, can achieve better than 1 cm resolution of distant rotating targets using range-Doppler imaging. To do this, we have produced trains of 50 ps mode-locked pulses with less than 25 kHz peak-to-peak optical frequency broadening and jitter of the laser modes. This frequency stability is achieved by active control of the oscillator cavity length using an external cavity as a reference. Cavity length stabilization can also reduce mode-locked laser timing jitter if the jitter is caused by cavity optical path length changes common to all laser modes. In our laser, however, the active optical-frequency-stabilization did not significantly improve laser pulse timing stability from the approximate 2-ps jitter levels achieved in our passively stabilized cavity. Analysis of the data indicates that a significant fraction of the timing jitter was due to laser cavity path length changes that varied from mode to mode     

CCR5/delta(ccr5) heterozygosity: a selective pressure for the syncytium-inducing human immunodeficiency virus type 1 phenotype. NIAID AIDS Clinical Trials Group Protocol 241 Virology Team

Mechanisms underlying the delay in dominance of syncytium-inducing (SI) phenotype HIV-1 (human immunodeficiency virus type 1) in vivo are unknown. Both random mutational events and selective pressures operative only late in the disease process have been suggested to underlie the shift from CCR5 to alternative coreceptor usage. Among the moderately advanced patients who entered AIDS Clinical Trials Group protocol 241, SI viral phenotype was more common among CCRS/delta(ccr5) heterozygotes (7/7, 100%) than among CCR5/CCR5 homozygotes (29/88, 33%; P < .001, Fisher's exact test). Other characteristics did not differ at study entry by CCR5 genotype, including median CD4 cell counts, plasma RNA levels, and infectious HIV-1 titers in circulating cells. These data indicate that CCR5/delta(ccr5) heterozygosity, which decreases cell-surface levels of CCR5 available to serve as an HIV-1 entry coreceptor, is a selective pressure for evolution of T cell line-tropic viruses that use an alternative coreceptor.     

Laboratory abnormalities in thrombotic thrombocytopenic purpura. Canadian Apheresis Group

Thrombotic thrombocytopenic purpura is an uncommon disorder that requires prompt recognition and intervention to prevent death. To date, information regarding the classic laboratory abnormalities in the disease has been derived from small numbers of patients whose laboratory tests have been done at many different sites. We report the laboratory findings in 135 patients who presented with thrombotic thrombocytopenic purpura to 17 Canadian centres. 50 men and 85 women had a mean platelet count of 25.3+/-19.4x10(9)/l. The initial platelet count correlated with mortality; 32% of patients with a platelet count of 20x10(9)/l or less died compared with 18% of patients with a platelet count >20x10(9)/l (P=0.058). The platelet-associated IgG was elevated in 88% at presentation whereas the indirect platelet suspension immunofluorescence test was positive in only 18%, 93% of the sera showed reactivity against platelets following protein blotting. All sera tested also showed reactivity against endothelial cells. Immune complexes were seen in all patients, whereas the platelet aggregating factor was detected in 59%. Although the von Willebrand factor was elevated in the majority of patients at entry, the multimer pattern was variable and showed no predictive pattern. Renal dysfunction was common (18%).     

Assessment of regional left ventricular long-axis motion with MR velocity mapping in healthy subjects

We present a case of trichothiodystrophy associated with a hypereosinophilic syndrome. This case had been followed for 30 years. Trichothiodystrophy was characterized by lamellar ichthyosis, hair and nail dystrophies, kyphoscoliosis, congenital luxation of the hip. The hypereosinophilic syndrome was characterized by an itching urticaria-like eruption. Although the patient's general condition had remaining stable for 30 years, during the last year it worsened and the patient suddenly died. The authors discuss about the significance of this association.     

Identification of myoglobin in human smooth muscle

Myoglobin (Mb) has been believed to be absent generally from mammalian smooth muscle tissue. Examination of human rectal, uterine, bladder, colon, small intestine, arterial, and venous smooth muscle by immunohistochemical techniques shows that each of these tissues is immunopositive for both smooth muscle myosin and human Mb. Mb-specific primers were used for the polymerase chain reaction to generate cDNA from smooth muscle tissues. Southern hybridization with a Mb-specific probe gave a very strong signal with the cDNA from rectum, weaker signals from small intestine and uterus, a faint signal from colon, and no signal from bladder tissue. High performance liquid chromatography analysis coupled with sequence determination has shown that contaminating heme-binding serum albumin as well as hemoglobin in extracts of smooth muscle seriously compromise any heme-based or spectrophotometric assay of Mb. Combined affinity and size exclusion chromatography, however, provide the necessary resolution. The cDNA-derived amino acid sequence of human smooth muscle Mb was found to be identical to that of Mb from striated muscle.