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61.
Lipochitin Oligosaccharides Immobilized through Oximes in Glycan Microarrays Bind LysM Proteins 下载免费PDF全文
Dr. Nicolai N. Maolanon Dr. Mickael Blaise Dr. Kasper K. Sørensen Prof. Mikkel B. Thygesen Dr. Emiliano Cló Dr. John T. Sullivan Prof. Clive W. Ronson Prof. Jens Stougaard Prof. Ola Blixt Prof. Knud J. Jensen 《Chembiochem : a European journal of chemical biology》2014,15(3):425-434
Glycan microarrays have emerged as novel tools to study carbohydrate–protein interactions. Here we describe the preparation of a covalent microarray with lipochitin oligosaccharides and its use in studying proteins containing LysM domains. The glycan microarray was assembled from glycoconjugates that were synthesized by using recently developed bifunctional chemoselective aminooxy reagents without the need for transient carbohydrate protecting groups. We describe for the first time the preparation of a covalent microarray with lipochitin oligosaccharides and its use for studying proteins containing LysM domains. Lipochitin oligosaccharides (also referred to as Nod factors) were isolated from bacterial strains or chemoenzymatically synthesized. The glycan microarray also included peptidoglycan‐related compounds, as well as chitin oligosaccharides of different lengths. In total, 30 ligands were treated with the aminooxy linker molecule. The identity of the glycoconjugates was verified by mass spectrometry, and they were then immobilized on the array. The presence of the glycoconjugates on the array surface was confirmed by use of lectins and human sera (IgG binding). The functionality of our array was tested with a bacterial LysM domain‐containing protein, autolysin p60, which is known to act on the bacterial cell wall peptidoglycan. P60 showed specific binding to Nod factors and to chitin oligosaccharides. Increasing affinity was observed with increasing chitin oligomer length. 相似文献
62.
For the life-cycle analysis (LCA) of deteriorating engineering systems, it is critical to model and incorporate the various deterioration processes and associated uncertainties. This paper proposes a renewal-theory life-cycle analysis (RTLCA) with state-dependent stochastic models (SDSMs) that describe the deterioration processes. The SDSMs capture the multiple deterioration processes and their interactions through modelling the changes in the system state variables due to different deterioration processes. Then proper capacity and demand models that take the time-variant state variables as input are adopted to fully capture the impact of deterioration processes on the capacity, demand, and other time-variant performance indicators of the engineering system. The SDSMs are then integrated into RTLCA to efficiently evaluate various life-cycle performance quantities such as availability, operation cost and benefits of the engineering system. To implement the proposed formulation, a sampling-based approach is adopted to simulate samples from the relevant probability density functions (PDFs) to estimate the life-cycle performance quantities, while stochastic simulation-based approach is adopted to estimate the time-variant performance indicators needed to inform intervention activities. As an illustration, the proposed formulation is used to analyse the life-cycle performances of an example reinforced concrete bridge subject to deterioration due to corrosion and seismic loading. 相似文献
63.
Patrick Rozier Mathieu Morcrette Olivier Szajwaj Vincent Bodenez Mickael Dolle Christine Surcin Loic Dupont Jean Marie Tarascon 《Israel journal of chemistry》2008,48(3-4):235-249
The present paper is in part a continuation of our recent work dealing with the fully reversible Li-driven Cu extrusion/injection of Cu in Cu2.33V4O11. The peculiar electrochemical property of such a compound was mainly ascribed to both structural considerations (presence of anchoring oxygen, giving flexibility to the [V4O11] layers) and electronic considerations, such as the existence of a delicate balance between the two Cu+/Cu2+ and V4+/V5+ redox centers. To support such suggestions, numerous compounds were revisited for their electrochemical–structural interplay. Network dimensionality, lattice flexibility, and cationic mobility parameters were addressed by exploring in depth the richness of the Cu–V–O system, while the importance of the donor/acceptor energy levels was checked via the choice of compounds having the right energy match between the two redox centers. As an extensive trial-and-error approach was not realistic, we relied on simple considerations based on the ionization energy of the various d-metals to narrow down metal pairs having redox levels occurring within the same range of energies. Bearing this in mind, it was logical to consider compounds still containing Cu ions but with Nb instead of V or compounds having V and Ag instead of Cu. Regardless of our effort, it turned out that the number of potential candidates worth considering for practical applications is still very limited, in spite of the elegance of this new Li reactivity mechanism. 相似文献
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65.
Nicolas Delpouve Mickael Arnoult Allisson Saiter Eric Dargent Jean‐Marc Saiter 《Polymer Engineering and Science》2014,54(5):1144-1150
Amorphous phase dynamics in Poly(lactic acid) (PLA) with different crystallinity degrees have been investigated from the vitreous state to the glass transition by means of two calorimetric methods. Temperature Modulated Differential Scanning Calorimetry was used to characterize the heat capacity signals and the average cooperativity length at the glass transition in non‐aged materials. Standard DSC was used to study the physical aging. It is shown that amorphous and fully crystallized PLA exhibit different relaxation parameters. For semicrystalline PLA with an intermediate degree of crystallinity, the peaks of the enthalpy of recovery and the out‐of‐phase heat capacity component are bimodal. The bimodality of the peaks is attributed to the relaxations of the inter‐spherulitic and intra‐spherulitic amorphous phases, respectively. Thus, in partially crystallized PLA, the non‐crystalline fraction of the material could be divided in three fractions, namely the Rigid Amorphous Fraction, the inter‐spherulitic Mobile Amorphous Phase (MAP), and the intra‐spherulitic MAP. Each of them exhibits a distinct molecular mobility. POLYM. ENG. SCI., 54:1144–1150, 2014. © 2013 Society of Plastics Engineers 相似文献
66.
Rémy Boulesteix Adrian Goldstein Camille Perrière Alexandre Maître Mickael Katz Christophe Coureau Christian Sallé 《Journal of the European Ceramic Society》2021,41(3):2085-2095
This paper presents experimental data and its interpretation regarding the forming of YAG and spinel green-bodies, intended for transparent parts fabrication, by the pressure slip casting (PSC) method. Conditions for an optimal operation are established based on the modeling of the filtration kinetics. It emerges that the method is able to provide highly sinterable green parts by ensuring that the cakes porosity exhibits low average size and narrow size distribution. Results were compared with other popular forming approaches like slip casting (SC) and cold isostatic pressing (CIP). PSC was found as superior, to the other approaches, as far as obtainment of high sinterability green- bodies is concerned. In the case of YAG, it was shown that PSC method even allows the replacement of the traditional long vacuum firings by a two stage densification operation in which an initial air-firing is completed by a hot isostatic pressing step. 相似文献
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68.
Arnal B Pernot M Tanter M 《IEEE transactions on ultrasonics, ferroelectrics, and frequency control》2011,58(2):369-378
The clinical applicability of high-intensity focused ultrasound (HIFU) for noninvasive therapy is today hampered by the lack of robust and real-time monitoring of tissue damage during treatment. The goal of this study is to show that the estimation of local tissue elasticity from shear wave imaging (SWI) can lead to the 2-D mapping of temperature changes during HIFU treatments. This new concept of shear wave thermometry is experimentally implemented here using conventional ultrasonic imaging probes. HIFU treatment and monitoring were, respectively, performed using a confocal setup consisting of a 2.5-MHz single-element transducer focused at 30 mm on ex vivo samples and an 8-MHz ultrasound diagnostic probe. Thermocouple measurements and ultrasound-based thermometry were used as a gold standard technique and were combined with SWI on the same device. The SWI sequences consisted of 2 successive shear waves induced at different lateral positions. Each wave was created using 100-μs pushing beams at 3 depths. The shear wave propagation was acquired at 17,000 frames/s, from which the elasticity map was recovered. HIFU sonications were interleaved with fast imaging acquisitions, allowing a duty cycle of more than 90%. Elasticity and temperature mapping was achieved every 3 s, leading to realtime monitoring of the treatment. Tissue stiffness was found to decrease in the focal zone for temperatures up to 43°C. Ultrasound-based temperature estimation was highly correlated to stiffness variation maps (r2 = 0.91 to 0.97). A reversible calibration phase of the changes of elasticity with temperature can be made locally using sighting shots. This calibration process allows for the derivation of temperature maps from shear wave imaging. Compared with conventional ultrasound-based approaches, shear wave thermometry is found to be much more robust to motion artifacts. 相似文献
69.
Arnal B Pernot M Tanter M 《IEEE transactions on ultrasonics, ferroelectrics, and frequency control》2011,58(8):1603-1611
The clinical applicability of high-intensity focused ultrasound (HIFU) for noninvasive therapy is currently hampered by the lack of robust and real-time monitoring of tissue damage during treatment. The goal of this study is to show that the estimation of local tissue elasticity from shear wave imaging (SWI) can lead to a precise mapping of the lesion. HIFU treatment and monitoring were respectively performed using a confocal setup consisting of a 2.5-MHz single element transducer focused at 34 mm on ex vivo samples and an 8-MHz ultrasound diagnostic probe. Ultrasound-based strain imaging was combined with shear wave imaging on the same device. The SWI sequences consisted of 2 successive shear waves induced at different lateral positions. Each wave was created with pushing beams of 100 μs at 3 depths. The shear wave propagation was acquired at 17,000 frames/s, from which the elasticity map was recovered. HIFU sonications were interleaved with fast imaging acquisitions, allowing a duty cycle of more than 90%. Thus, elasticity and strain mapping was achieved every 3 s, leading to real-time monitoring of the treatment. When thermal damage occurs, tissue stiffness was found to increase up to 4-fold and strain imaging showed strong shrinkages that blur the temperature information. We show that strain imaging elastograms are not easy to interpret for accurate lesion characterization, but SWI provides a quantitative mapping of the thermal lesion. Moreover, the concept of shear wave thermometry (SWT) developed in the companion paper allows mapping temperature with the same method. Combined SWT and shear wave imaging can map the lesion stiffening and temperature outside the lesion, which could be used to predict the eventual lesion growth by thermal dose calculation. Finally, SWI is shown to be robust to motion and reliable in vivo on sheep muscle. 相似文献