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31.
Synthetic biology depends on the ability to rapidly produce strains with improved phenotypes but is limited by the ability to rapidly produce strain collections with directed mutations. Here, we present a system capable of overcoming this limitation through automated P1-phage transductions of Escherichia coli. By combining the Keio collection of single-gene deletion E. coli mutants with P1-phage, it is possible to generate an engineered host-strain collection consisting of every possible gene deletion mutant. This strategy was tested by transducing 355 genetic markers from the Keio collection into five different host strains, and it achieved a 98% success rate. This method offers an improved mechanism for rapidly engineering collections of microbes and provides one method for rapidly deploying a broader synthetic biology effort. 相似文献
32.
33.
Sónia M. Almeida-Luz Miguel A. Vega-Rodríguez Juan A. Gómez-Púlido Juan M. Sánchez-Pérez 《Applied Soft Computing》2011,11(1):410-427
In this work we present two new approaches to solve the location management problem, respectively, based on the location areas and the reporting cells strategies. The location management problem corresponds to the management of the network configuration with the objective of minimizing the costs involved. We use the differential evolution algorithm to find the best configuration for the location areas and the reporting cells strategies, which principally considers the location update and paging costs. With this work we want to define the best values to the differential evolution configuration, using test networks and also realistic networks, as well as compare our results with the ones obtained by other authors. These two new approaches applied to this problem have given us very good results, when compared with those obtained by other authors. 相似文献
34.
Ignacio Algredo-Badillo Miguel Morales-Sandoval 《Computers & Electrical Engineering》2010,36(3):565-577
Applications of wireless communications networks are emerging continuously. To offer a good level of security in these applications, new standards for wireless communications propose solutions based on cryptographic algorithms working on special modes of operation. This work presents a custom hardware architecture for the AES-CCM protocol (AES-CCMP) which is the basis for the security architecture of the IEEE 802.11i standard. AES-CCMP is based on the AES-CCM algorithm that performs the Advanced Encryption Standard (AES) in CTR with CBC-MAC mode (CCM mode), plus specialized data formatting modules, providing different security services through iterative and complex operations. Results of implementing the proposed architecture targeting FPGA devices are presented and discussed. A comparison against similar works shows significant improvements in terms of both throughput and efficiency. 相似文献
35.
Soledad Escolar Díaz Florin Isaila Alejandro Calderón Mateos Luis Miguel Sanchez García David E. Singh 《The Journal of supercomputing》2010,51(1):76-93
In the last years the Wireless Sensor Networks’ (WSN) technology has been increasingly employed in various application domains.
The extensive use of WSN posed new challenges in terms of both scalability and reliability. This paper proposes Sensor Node
File System (SENFIS), a novel file system for sensor nodes, which addresses both scalability and reliability concerns. SENFIS
can be mainly used in two broad scenarios. First, it can transparently be employed as a permanent storage for distributed
TinyDB queries, in order to increase the reliability and scalability. Second, it can be directly used by a WSN application
for permanent storage of data on the WSN nodes. The experimental section shows that SENFIS implementation makes an efficient
use of resources in terms of energy consumption, memory footprint, flash wear levelling, while achieving execution times similarly
with existing WSN file systems. 相似文献
36.
Pablo Quintía Roberto Iglesias Miguel A. Rodríguez Carlos V. Regueiro 《Robotics and Autonomous Systems》2010,58(12):1306-1315
One of the main problems of robots is the lack of adaptability and the need for adjustment every time the robot changes its working place. To solve this, we propose a learning approach for mobile robots using a reinforcement-based strategy and a dynamic sensor-state mapping. This strategy, practically parameterless, minimises the adjustments needed when the robot operates in a different environment or performs a different task.Our system will simultaneously learn the state space and the action to execute on each state. The learning algorithm will attempt to maximise the time before a robot failure in order to obtain a control policy suited to the desired behaviour, thus providing a more interpretable learning process. The state representation will be created dynamically, starting with an empty state space and adding new states as the robot finds new situations that has not seen before. A dynamic creation of the state representation will avoid the classic, error-prone and cyclic process of designing and testing an ad hoc representation. We performed an exhaustive study of our approach, comparing it with other classic strategies. Unexpectedly, learning both perception and action does not increase the learning time. 相似文献
37.
Miguel Juliá Óscar Reinoso Arturo Gil Mónica Ballesta Luis Payá 《Engineering Applications of Artificial Intelligence》2010,23(4):473-486
In this paper we present a hybrid reactive/deliberative approach to the multi-robot integrated exploration problem. In contrast to other works, the design of the reactive and deliberative processes is exclusively oriented to the exploration having both the same importance level. The approach is based on the concepts of expected safe zone and gateway cell. The reactive exploration of the expected safe zone of the robot by means of basic behaviours avoids the presence of local minima. Simultaneously, a planner builds up a decision tree in order to decide between exploring the current expected safe zone or changing to other zone by means of travelling to a gateway cell. Furthermore, the model takes into account the degree of localization of the robots to return to previously explored areas when it is necessary to recover the certainty in the position of the robots. Several simulations demonstrate the validity of the approach. 相似文献
38.
Jose M. Chaves-González Miguel A. Vega-Rodríguez Juan A. Gómez-Pulido Juan M. Sánchez-Pérez 《Digital Signal Processing》2010,20(3):806-823
Skin colour detection is a technique very used in most of face detectors to find faces in images or videos. However, there is not a common opinion about which colour space is the best choice to do this task. Therefore, the motivation for our study is to discover which colour model is the best option to build an efficient face detector which can be embedded in a functional face recognition system. We have studied 10 of the most common and used colour spaces doing different comparisons among them, in order to know which one is the best option for human skin colour detection. In concrete, we have studied the models: RGB, CMY, YUV, YIQ, YPbPr, YCbCr, YCgCr, YDbDr, HSV—or HSI—and CIE-XYZ. To make the comparison among them, we have used 15 truth images where the skin colour of a face is clearly separated from the rest of the image (background, eyes, lips, hair, etc.). Thus we can compare at level pixel each colour model, doing a detailed study of each format. We present the final conclusions comparing different results, such as: right detections, false positives and false negatives for each colour space. According to the obtained results, the most appropriate colour spaces for skin colour detection are HSV model (the winner in our study), and the models YCgCr and YDbDr. 相似文献
39.
Brain-machine interfaces (BMIs) transform the activity of neurons recorded in motor areas of the brain into movements of external actuators. Representation of movements by neuronal populations varies over time, during both voluntary limb movements and movements controlled through BMIs, due to motor learning, neuronal plasticity, and instability in recordings. To ensure accurate BMI performance over long time spans, BMI decoders must adapt to these changes. We propose the Bayesian regression self-training method for updating the parameters of an unscented Kalman filter decoder. This novel paradigm uses the decoder's output to periodically update its neuronal tuning model in a Bayesian linear regression. We use two previously known statistical formulations of Bayesian linear regression: a joint formulation, which allows fast and exact inference, and a factorized formulation, which allows the addition and temporary omission of neurons from updates but requires approximate variational inference. To evaluate these methods, we performed offline reconstructions and closed-loop experiments with rhesus monkeys implanted cortically with microwire electrodes. Offline reconstructions used data recorded in areas M1, S1, PMd, SMA, and PP of three monkeys while they controlled a cursor using a handheld joystick. The Bayesian regression self-training updates significantly improved the accuracy of offline reconstructions compared to the same decoder without updates. We performed 11 sessions of real-time, closed-loop experiments with a monkey implanted in areas M1 and S1. These sessions spanned 29 days. The monkey controlled the cursor using the decoder with and without updates. The updates maintained control accuracy and did not require information about monkey hand movements, assumptions about desired movements, or knowledge of the intended movement goals as training signals. These results indicate that Bayesian regression self-training can maintain BMI control accuracy over long periods, making clinical neuroprosthetics more viable. 相似文献
40.
Luca Carlone Miguel Kaouk Ng Jingjing Du Basilio Bona Marina Indri 《Journal of Intelligent and Robotic Systems》2011,63(2):283-307
In this paper we investigate the problem of Simultaneous Localization and Mapping (SLAM) for a multi robot system. Relaxing some assumptions that characterize related work we propose an application of Rao-Blackwellized Particle Filters (RBPF) for the purpose of cooperatively estimating SLAM posterior. We consider a realistic setup in which the robots start
from unknown initial poses (relative locations are unknown too), and travel in the environment in order to build a shared
representation of the latter. The robots are required to exchange a small amount of information only when a rendezvous event
occurs and to measure relative poses during the meeting. As a consequence the approach also applies when using an unreliable
wireless channel or short range communication technologies (bluetooth, RFId, etc.). Moreover it allows to take into account
the uncertainty in relative pose measurements. The proposed technique, which constitutes a distributed solution to the multi
robot SLAM problem, is further validated through simulations and experimental tests. 相似文献