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71.
Adriana Marinoiu Elena Carcadea Ada Sacca Alessandra Carbone Claudia Sisu Andreea Dogaru Mircea Raceanu Mihai Varlam 《International Journal of Hydrogen Energy》2021,46(22):12242-12253
Here in, we describe an ultrafast, single-step microwave irradiation route (MW) to prepare graphene supported Pt nanoparticles, during which the small Pt nanoparticles are distributed uniformly on a reduced graphene oxide surface. This route provides evident advantages namely low cost, easiness, low time consuming and high yield in comparison to actual chemical methods to develop efficient Pt/rGO catalyst with Pt content close to state-of-the-art commercial composition. The structure and composition of prepared samples have been studied by specific techniques, while the electrocatalytic stability has been studied using ex-situ and in-situ measurements. High performance and electrochemically stable catalyst for PEM fuel cells was developed using the sample with highest loading and good dispersion. The fabricated Pt-rGO-based MEA was investigated for durability under fuel starvation in comparison with commercial Pt/C-based MEA. The electrocatalytic activity was investigated and the electrochemical response revealed the higher stability during accelerated degradation test under fuel starvation in comparison with commercial Pt/C. This study promotes the applicability of described preparation method to noble or transition metal nanoparticles embedded on graphene-based materials. 相似文献
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Water Resources Management - Improving drinking water source monitoring is crucial for efficiently managing the drinking water treatment process and ensuring the delivery of safe water. Data mining... 相似文献
74.
Liu Y Mihai C Kubiak RJ Rebecchi M Bruzik KS 《Chembiochem : a European journal of chemical biology》2007,8(12):1430-1439
Accurate measurement of phosphatidylinositol-specific phospholipase C (PI-PLC) activity is important in view of the key role of this enzyme in signal-transduction pathways. In this work we synthesized enantiomerically pure phosphorothiolate analogues of all natural PI-PLC substrates, including those of phosphatidylinositol 4,5-bisphosphate (PI-4,5-P2), 4-phosphate (PI-4-P), 5-phosphate (PI-5-P) and unphosphorylated PI, in both long- and short-chain versions. The enzymatic cleavage of these substrates produces thiol analogues of diacyl glycerol, which can be quantified by UV absorbance after treatment with dipyridyl disulfide. The monodisperse dihexanoyl derivatives are suitable substrates for PI-PLC assay: they give rise to high enzyme activity, and provide excellent linear kinetic responses. For all substrates, we found a good linear correlation between the reaction rate and the amount of enzyme; this indicated the suitability of this assay for enzyme quantification. The short-chain substrates enable the enzyme specificity with variously phosphorylated inositol head groups to be established--unobstructed by substrate aggregation, "scooting" kinetics on micelles, or surface dilution effects. The kinetic results indicated allosteric behavior of PLC for all substrates tested. We found that substrates phosphorylated at the inositol 4-position (phosphorothiolate analogues of PI-4,5-P2 and PI-4-P) displayed very similar kinetic properties, and were cleaved with approximately 20- to 30-fold higher activity than the 4-nonphosphorylated substrates (analogues of PI-5-P and PI). Hence it appears that interactions between the enzyme and the 4-phosphate group of the substrate, but not its 5-phosphate group, is important for PI-PLC catalysis. In addition, the binding affinities of all four substrate types were found to be quite similar; this indicates that the energy of enzyme interaction with the 4-phosphate group is directed almost entirely to catalysis. 相似文献
75.
Thomas Bak Angus Graham Alla Sapronova Mihai Florian John Dalsgaard Sørensen Torben Knudsen Peng Hou Zhe Chen 《风能》2017,20(9):1665-1683
The paper presents a model of a reference wind farm. The model considers the wind and wave climatologies for a specific site from which two different wind farm layouts are derived. These layouts are examined through the effective wake‐enhanced turbulence intensity at the hub height for a given climatology, and a simple model for the influence on capital expenditures is proposed. An electrical design is presented, the cable losses are calculated and the energy yield is determined. An operation and maintenance model is established, and the associated operating expenditure is obtained. All of the models are then summarized in terms of a levelized cost of energy using a numerical simulation tool, which allows the layouts to be compared. The data and models are freely available online for others to use and may serve as a baseline for benchmarking and allow researchers to compare and discuss their results. Copyright © 2017 John Wiley & Sons, Ltd. 相似文献
76.
Masaharu Takeda Hiroyuki Hiraishi Toshikazu Takesako Sumio Tanase Norio Gunge 《Yeast (Chichester, England)》1996,12(3):241-246
The 36K protein attached at the 5′ end of the linear DNA plasmid pGKL2 from the yeast Kluyveromyces lactis was first purified and characterized. The terminal protein was purified from cells (1 kg wet weight) by ammonium sulphate precipitation and two rounds of centrifugation to equilibrium in CsCl gradients. The pGKL2 was present only in the post-microsomal supernatant. Approximately 10 mg of the purified pGKL2 was recovered and digested with DNase I. The terminal protein (final ca. 0·8 mg) was homogeneous by electrophoresis and we determined the N-terminal amino acid sequence up to ten residues, showing that it existed in the cryptic N-terminal domain of pGKL2-ORF2 (DNA polymerase) sequence. 相似文献
77.
Stefania Racovita Marin-Aurel Trofin Diana Felicia Loghin Marius-Mihai Zaharia Florin Bucatariu Marcela Mihai Silvia Vasiliu 《International journal of molecular sciences》2021,22(17)
Polybetaines, that have moieties bearing both cationic (quaternary ammonium group) and anionic groups (carboxylate, sulfonate, phosphate/phosphinate/phosphonate groups) situated in the same structural unit represent an important class of smart polymers with unique and specific properties, belonging to the family of zwitterionic materials. According to the anionic groups, polybetaines can be divided into three major classes: poly(carboxybetaines), poly(sulfobetaines) and poly(phosphobetaines). The structural diversity of polybetaines and their special properties such as, antifouling, antimicrobial, strong hydration properties and good biocompatibility lead to their use in nanotechnology, biological and medical fields, water remediation, hydrometallurgy and the oil industry. In this review we aimed to highlight the recent developments achieved in the field of biomedical applications of polybetaines such as: antifouling, antimicrobial and implant coatings, wound healing and drug delivery systems. 相似文献
78.
Zikov T Bibian S Dumont GA Huzmezan M Ries CR 《IEEE transactions on bio-medical engineering》2006,53(4):617-632
This paper reports on a novel method for quantifying the cortical activity of a patient during general anesthesia as a surrogate measure of the patient's level of consciousness. The proposed technique is based on the analysis of a single-channel (frontal) electroencephalogram (EEG) signal using stationary wavelet transform (SWT). The wavelet coefficients calculated from the EEG are pooled into a statistical representation, which is then compared to two well-defined states: the awake state with normal EEG activity, and the isoelectric state with maximal cortical depression. The resulting index, referred to as the wavelet-based anesthetic value for central nervous system monitoring (WAV(CNS)), quantifies the depth of consciousness between these two extremes. To validate the proposed technique, we present a clinical study which explores the advantages of the WAV(CNS) in comparison with the BIS monitor (Aspect Medical Systems, MA), currently a reference in consciousness monitoring. Results show that the WAV(CNS) and BIS are well correlated (r = 0.969) during periods of steady-state despite fundamental algorithmic differences. However, in terms of dynamic behavior, the WAV(CNS) offers faster tracking of transitory changes at induction and emergence, with an average lead of 15-30 s. Furthermore, and conversely to the BIS, the WAV(CNS) regains its preinduction baseline value when patients are responding to verbal command after emergence from anesthesia. We conclude that the proposed analysis technique is an attractive alternative to BIS monitoring. In addition, we show that the WAV(CNS) dynamics can be modeled as a linear time invariant transfer function. This index is, therefore, well suited for use as a feedback sensor in advisory systems, closed-loop control schemes, and for the identification of the pharmacodynamic models of anesthetic drugs. 相似文献
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