Dependence of apparent diffusion coefficients on axonal spacing, membrane permeability, and diffusion time in spinal cord white matter

Paraneoplastic pemphigus (PNP) is an autoimmune blistering disease that occurs in association with underlying neoplasms. Patients with PNP develop characteristic IgG autoantibodies directed against multiple antigens, most of which have been identified as cytoplasmic proteins of the plakin family (desmoplakin I, II, BPAG1, envoplakin, and periplakin). This study identified cell surface target antigens of PNP. We focused on desmoglein (Dsg) 3 and Dsg1, the autoantigens of pemphigus vulgaris and pemphigus foliaceus. ELISA using baculovirus-expressed recombinant Dsgs (rDsg3, rDsg1) has revealed that 25 out of 25 PNP sera tested were positive against Dsg3 and 16 of 25 were positive against Dsg1. All of 12 PNP sera tested immunoprecipitated Dsg3. Removal of anti-Dsg3 autoantibodies by immunoadsorption was sufficient to eliminate the ability of PNP sera to induce cutaneous blisters in neonatal mice in vivo. Furthermore, anti-Dsg3-specific antibodies that were affinity purified from PNP sera were proven to be pathogenic and caused blisters in neonatal mice. These findings indicate that Dsg3 and Dsg1 are the cell surface target antigens in PNP and that IgG autoantibodies against Dsg3 in PNP sera play a pathogenic role in inducing loss of cell adhesion of keratinocytes and causing blister formation.     

既能防水又能透气的机壳

在设计高性能电子系统时,工程师都明白进行器件保护是非常重要的问题,既要保护其免受干扰又要保护敏感电子器件不被损坏。随着人类越来越依赖电子系统,保护措施对于系统可靠性和使用寿命的保证变得至关重要。敏感且昂贵的电子系统的应用环境也大大拓展了。长期以来,电子系统已经被广泛用于从舰船技术到食品生产设备等几乎一切领域之中,而近年来无线通信设备的普及是电子系统应用扩展过程中的最新进展,甚至包括刮胡刀和牙刷在内的消费产品也需要保护其中的敏感元器件。然而,工程师发现的问题是,对于设备外壳(或机壳)而言,既需要阻止或防止外…     

Patient-controlled sedation and analgesia, using propofol and alfentanil, during colonoscopy: a prospective randomized controlled trial

BACKGROUND AND STUDY AIMS: Patient-controlled sedation (PCS) enables titration of dosage to an individual's requirements and is potentially useful in colonoscopy. The aim was to compare the value of patient-controlled sedation, using propofol and alfentanil, with that of intravenous diazemuls and pethidine during colonoscopy. PATIENTS AND METHODS: Following randomization, 66 patients undergoing colonoscopy received either an intravenous bolus of pethidine (50 mg) and diazemuls (10-20 mg) prior to colonoscopy or were connected to an infusion pump containing propofol (10 mg/ml) and alfentanil (25 microg/ml). Patients self-administered 0.5 ml boluses as often as they required. Pain and sedation score were recorded by a nurse specialist and on a patient questionnaire. An anaesthetist was present throughout the procedure. RESULTS: PCS provided lighter sedation (median sedation score, 3 versus 4; P=0.0001), less analgesia (median pain score, 1 versus 0; P=0.004), a smaller maximum fall in systolic blood pressure (23 mmHg versus 33 mmHg; P=0.02) and a faster recovery time (median 10 min versus 40 min; P=0.0001), irrespective of the dose administered, compared with a diazemuls-pethidine combination. The duration of the procedure was unaffected. All patients were satisfied with their level of sedation. CONCLUSIONS: Patient-controlled sedation is an effective alternative to premedication with narcotic/benzodiazepine combinations during colonoscopy.     

Local surface shape estimation of 3-D textured surfaces usingGaussian Markov random fields and stereo windows

The problem of extracting the local shape information of a 3-D texture surface from a single 2-D image by tracking the perceived systematic deformations the texture undergoes by virtue of being present on a 3-D surface and by virtue of being imaged is examined. The surfaces of interest are planar and developable surfaces. The textured objects are viewed as originating by laying a rubber planar sheet with a homogeneous parent texture on it onto the objects. The homogeneous planar parent texture is modeled by a stationary Gaussian Markov random field (GMRF). A probability distribution function for the texture data obtained by projecting the planar parent texture under a linear camera model is derived, which is an explicit function of the parent GMRF parameters, the surface shape parameters. and the camera geometry. The surface shape parameter estimation is posed as a maximum likelihood estimation problem. A stereo-windows concept is introduced to obtain a unique and consistent parent texture from the image data that, under appropriate transformations, yields the observed texture in the image. The theory is substantiated by experiments on synthesized as well as real images of textured surfaces     

Spatially controlled cell engineering on biodegradable polymer surfaces

Controlling receptor-mediated interactions between cells and template surfaces is a central principle in many tissue engineering procedures (1-3). Biomaterial surfaces engineered to present cell adhesion ligands undergo integrin-mediated molecular interactions with cells (1, 4, 5), stimulating cell spreading, and differentiation (6-8). This provides a mechanism for mimicking natural cell-to-matrix interactions. Further sophistication in the control of cell interactions can be achieved by fabricating surfaces on which the spatial distribution of ligands is restricted to micron-scale pattern features (9-14). Patterning technology promises to facilitate spatially controlled tissue engineering with applications in the regeneration of highly organized tissues. These new applications require the formation of ligand patterns on biocompatible and biodegradable templates, which control tissue regeneration processes, before removal by metabolism. We have developed a method of generating micron-scale patterns of any biotinylated ligand on the surface of a biodegradable block copolymer, polylactide-poly(ethylene glycol). The technique achieves control of biomolecule deposition with nanometer precision. Spatial control over cell development has been observed when using these templates to culture bovine aortic endothelial cells and PC12 nerve cells. Furthermore, neurite extension on the biodegradable polymer surface is directed by pattern features composed of peptides containing the IKVAV sequence (15, 16), suggesting that directional control over nerve regeneration on biodegradable biomaterials can be achieved.     

Nonhydrostatic Three-Dimensional Method for Hydraulic Flow Simulation. II: Validation and Application

A three-dimensional computational method, without the use of hydrostatic assumption, is developed to solve fluid flows for hydraulic applications. Numerical algorithms and verification of the nonhydrostatic model are described in our companion paper. The model employs unstructured grid technology with arbitrarily shaped cells, offering the potential to unify many grid topologies into a single formulation. Herein, the model is applied to two practical steady hydraulic flows to provide further validation of the model and demonstrate its use in practical flows. The flows in a hydroturbine draft tube and in the forebay of Rocky Reach Dam for the fish passage facility design are simulated. Comparisons with experimental data in the former and physical and field measurements in the latter establish the scope of the model.     

Visible emission from ZnO doped with rare-earth ions

We report the results of a cathodoluminescence (CL) and photoluminescence (PL) study of ZnO-bulk single crystals and epilayer thin-film samples grown on a sapphire (0001) substrate and doped by implantation with rare-earth ions (RE³⁺): Pr³⁺, Dy³⁺, Ho³⁺, Er³⁺, Tm³⁺ (bulk crystals, co-doped with Li), Sm³⁺, Dy³⁺, and Er³⁺ (epilayers). The PL and PL excitation (PLE) spectra of polycrystalline ZnO doped with RE³⁺ ions (Nd³⁺, Dy³⁺, Er³⁺, and Tm³⁺) and codoped with Li⁺, Cl⁻, and N⁻ ions have also been studied.     

Different effects of opiate withdrawal on dopamine turnover, uptake, and release in the striatum and nucleus accumbens

The purpose of these experiments was to further characterize changes in dopaminergic function that follow withdrawal from chronic opiate treatment. Withdrawal after treatment to a maximum dose of 120 mg/kg of morphine did not alter dopamine concentrations in the substantia nigra, ventral tegmental area, striatum, or nucleus accumbens; but did decrease concentrations of DOPAC and the ratio of DOPAC to dopamine in the lateral striatum and nucleus accumbens. Uptake of tritiated dopamine was diminished for withdrawn slices obtained from the striatum with no effect observed for tissue from the nucleus accumbens. Deficits of in vitro release of tritiated dopamine also occurred following withdrawal, with the nucleus accumbens being sensitive to dependence produced by a lower dose of morphine. In conclusion, opiate withdrawal produces a complex pattern of effects on dopaminergic function that is specific for the striatum and nucleus accumbens.     

Design and Implementation of a Composite Performance Evaluation Model for Heterogeneous Network Management Applications

This article examines the place of analytical models in realtime performance management applications. The main objectives are to define, specify and implement a system based on a composite model that provides the optimal software environment for performance analysis and tuning of heterogeneous networks.     

Mapping of genes predisposing to idiopathic generalized epilepsy

Idiopathic generalized epilepsy (IGE) is characterized by recurring generalized seizures in the absence of detectable brain lesions and/or metabolic abnormalities. Twin and family studies suggest that genetic factors play a key part in IGE. A multilocus model appears to best fit the observed inheritance patterns. Mapping of IGE-related genes has been previously attempted using parametric methods, with conflicting results. In particular, recent evidence argues both for and against a chromosome 6p locus (EJM1) for juvenile myoclonic epilepsy, a subtype of IGE. We have approached the problem of mapping IGE loci using non-parametric methods, which have recently been successful for other complex diseases. No evidence for linkage to chromosome 6p was obtained. However, we obtained evidence for involvement of a locus at chromosome 8q24, close to the marker D8S256. The same 8q24 region was previously implicated in families with benign neonatal familial convulsions (BNFC), a generalized epilepsy syndrome that is inherited as a simple dominant mendelian trait. There is an apparent conserved syntenic group of genes in human 8q24 and a region of mouse chromosome 15, which harbors the stargazer (stg) locus. Homozygous mutant mice at the stg locus show a form of generalized epilepsy that resembles human absence epilepsy. Our findings may have implications for a locus on 8q24 predisposing to IGE.