Characterization of rpoH in Acetobacter pasteurianus NBRC3283

The RpoH in Acetobacter pasteurianus NBRC3283 was characterized. It was revealed that the rpoH controls the expression of groEL, dnaKJ, grpE, and clpB to different extents. In addition, the rpoH disruption mutant became apt to be affected by heat, ethanol, and acetic acid, indicating its importance in acetic acid fermentation.     

CONVENTIONAL BASIC DESIGN FOR CONVECTION OR CONDUCTION DRYERS

Abstract

The items to be considered prior to selection of dryers are explained, and a simple method for a rough estimation of dryer sizes was proposed based on data obtained from operating industrial dryers.

The equations of basic design for batch or continuous type dryers were derived. The heat was supplied to materials by convection and/or conduction. The equations were simplified to the case when the falling rate of drying is proportional to the moisture content of materials under the constant drying conditions. The heat transfer coefficient used in the equations can be determined based on the calculations or the data obtained from the experimental or industrial dryers. The equations are useful for estimating the scale-up effect of dryers.     

The use of the block cycle length as a safe and efficient means of interrupting sustained ventricular tachycardia and its pharmacological modification

In nine patients who had inducible monomorphic sustained ventricular tachycardia (VT), rapid pacing was performed in 11 episodes of morphologically distinct VT at progressively shorter cycle lengths and VT was interrupted at a critical cycle length. The VT interrupting critical cycle length was defined as the block cycle length (BCL) and the effect of Class I antiarrhythmic drugs were examined. Both the VT cycle length (VTCL) and the BCL were prolonged after administration of either drug. The overall mean ratio of the BCL to the VTCL was unchanged after procainamide administration, but increased after the use of mexiletine. The ratio, however, varied in individual VTs and the BCL after treatment with Class I antiarrhythmic drugs could not be predicted from the ratio baseline value, although the ratio was always > 60% and the hazard of VT acceleration might be avoided if the BCL is used.     

Functional analysis of peripheral blood B cells in patients with X-linked agammaglobulinemia

X-linked agammaglobulinemia (XLA) is a primary immunodeficiency disease caused by mutations of Bruton tyrosine kinase (Btk); Btk plays an essential role in the development of mature B cells. However, small numbers of B cells ("leaky B cells") are present in the peripheral blood of most XLA patients. In this study, we analyzed the function of these leaky B cells obtained from XLA patients. Enough numbers of B cells were available for analysis from five of nine XLA patients originally screened. Sequence analysis revealed missense mutations of Btk in four of the five XLA patients. No mutation was found in the coding region of Btk in one patient. Western blotting and/or flow cytometric analysis failed to detect Btk protein in all five patients. B cells isolated from peripheral blood of these XLA patients were CD5-, CD20+, CD19+, and CD21-. If stimulated with anti-CD40 and IL-4, XLA B cells proliferated normally and produced significant amounts of IgE. Anti-CD40 stimulation of XLA B cells resulted in normal expression of CD23. In addition, three of the five XLA patients studied were immunized with bacteriophage phiX174 and produced low but detectable levels of antiphage-specific Ab. Similarly, X-linked immunodeficiency mice, which carry a missense mutation in Btk, produced substantial amounts of antiphage Ab. These results indicate that CD40 signaling is intact in B cells lacking demonstrable Btk, and that leaky B cells in XLA patients can proliferate, undergo isotype switching, and differentiate into specific Ab-producing cells.     

非吸湿多孔物料板块等温干燥速度的简捷算法

通过下列处理导出了非吸湿多孔物料板块等温干燥速度的简捷算法:(1)利用水分传递系数恒定的近似,通过解析传质偏微分方程得到表示干燥速度与平均湿含量关系的方程组;(2)把该方程组外推用于水传质系数可变的体系。该方法无需确定水传质系数与湿含量的关系,仅根据一条测定曲线即可预测任何等温干燥条件下的干燥速度曲线。用新的简捷算法计算的干燥速度曲线与偏微分方程的直接数值解吻合很好。     

In vitro and in vivo antimicrobial properties of silver-containing hydroxyapatite prepared via ultrasonic spray pyrolysis route

Hydroxyapatite (HAp), with its high biocompatibility and osteoconductivity, readily absorbs proteins, amino acids and other substances, which in turn favor the adsorption and colonization of bacteria. To prevent bacterial growth and biofilm formation on HAp discs, silver-containing (1–20 mol%) HAp (Ag-HAp) powders were synthesized using an ultrasonic spray pyrolysis (USSP) technique. The X-ray diffraction (XRD) peaks were very broad, indicating low crystallinity, and this induced the release of Ag⁺ ions from Ag-HAp powders. In addition, a gradual increase in Ca^2 + ion release was observed. These results suggest that dissolution of Ca^2 + ion in Ag-HAp triggered the release of Ag⁺ ions.The antimicrobial efficacy of Ag-HAp disc was tested against Staphylococcus aureus. Samples with Ag contents of more than 5 mol% were found to be highly effective against bacterial colonization and biofilm formation in vitro. In vivo antibacterial tests using bioluminescent strains also showed reductions in the viability of bacteria with Ag-HAp (5 mol%) discs. Biocompatibility tests using a modified Transwell® insert method showed that Ag-HAp (5 mol%) discs have negative effects on osteoblast proliferation. These results indicate that Ag-HAp (5 mol%) has effective antibacterial activity and good biocompatibility both in vitro and in vivo together with good biocompatibility, thus confirming its utility as a bactericidal material.     

Effect of Carrier Frequency and Circuit Resistance on Iron Loss of Non‐Oriented Electrical Steel Sheet under Single‐Phase Full‐Bridge PWM Inverter Excitation

Because of the development of power electronics technology, pulse width modulation (PWM) inverters are now being used to drive motors in order to achieve precise and energy‐efficient control. Precise estimation of the increase in iron loss due to the high harmonic components of flux, including the carrier frequency, is important in the design of motors excited by a PWM inverter. We measured the iron losses of non‐oriented electrical steel sheets that were excited using a single‐phase full‐bridge PWM inverter, and examined the influence of the carrier frequency and circuit resistance on the iron loss. We showed that the iron loss increased because of the generation of minor loops when the circuit resistance was high. Therefore, the circuit resistance should be decreased in an actual motor system.     

Design, synthesis, and in vitro antifungal activity of 1-[(4-substituted-benzyl)methylamino]-2-(2,4-difluorophenyl)-3-(1H-1,2,4-triazol-1-yl)propan-2-ols

As part of our studies focused on the design of 1‐[((hetero)aryl‐ and piperidinylmethyl)amino]‐2‐phenyl‐3‐(1H‐1,2,4‐triazol‐1‐yl)propan‐2‐ols as antifungal agents, we report the development of new extended benzylamine derivatives substituted at the para position by sulfonamide or retrosulfonamide groups linked to alkyl or aryl chains. These molecules have broad‐spectrum antifungal activities not only against Candida spp., including fluconazole‐resistant strains, but also against a filamentous species (A. fumigatus). Concerning fluconazole resistance, selected compounds exhibit the capacity to overcome CDR and ERG11 gene upregulation and to maintain antifungal activity despite a recognized critical CYP51 substitution in C. albicans isolates. Synthesis, investigation of the mechanism of action by sterol analysis in a C. albicans strain, and structure–activity relationships (SARs) are reported.