The interplay of cell–cell and cell–substrate adhesion in collective cell migration

Collective cell migration often involves notable cell–cell and cell–substrate adhesions and highly coordinated motion of touching cells. We focus on the interplay between cell–substrate adhesion and cell–cell adhesion. We show that the loss of cell-surface contact does not significantly alter the dynamic pattern of protrusions and retractions of fast migrating amoeboid cells (Dictyostelium discoideum), but significantly changes their ability to adhere to other cells. Analysis of the dynamics of cell shapes reveals that cells that are adherent to a surface may coordinate their motion with neighbouring cells through protrusion waves that travel across cell–cell contacts. However, while shape waves exist if cells are detached from surfaces, they do not couple cell to cell. In addition, our investigation of actin polymerization indicates that loss of cell-surface adhesion changes actin polymerization at cell–cell contacts. To further investigate cell–cell/cell–substrate interactions, we used optical micromanipulation to form cell–substrate contact at controlled locations. We find that both cell-shape dynamics and cytoskeletal activity respond rapidly to the formation of cell–substrate contact.     

Fault tolerance mechanisms for virtual data center architectures

A virtual data center (VDC) is a combination of interconnected virtual servers hosted on a physical data center that hosts multiple such VDCs. This enables efficient sharing of the data center’s resources while handling dynamic resource requirements of the clients. The SecondNet architecture (Guo et al. in Proceedings of ACMSIGCOMM conference on data communication, Barcelona, pp 63–74, 2009) realizes this VDC concept and includes a centralized VDC resource-mapping (virtual to physical) algorithm. Fault tolerance is an important requirement in data center-based services, in order to increase reliability and availability. In this paper, we propose a fault tolerance mechanism to handle server failures by efficiently migrating the virtual machines (VMs) hosted on the failed server to a new location. Using our mechanism, it is shown that recovery from all the faults is possible, even for a server utilization of 90 %. In order to reduce the impact of server failures on the VDCs hosted in the data center, we then present a new load balancing scheme based on clustering that efficiently allocates the VDCs on the data center. Using this scheme, we were able to reduce the affected number of VMs per server failure by 63 %, in case of a BCube network of size 625 nodes, and by 86 %, in case of a BCube network of size 1,296 nodes.     

Three optimizations for Assume–Guarantee reasoning with L<Superscript>*</Superscript>

The learning-based automated Assume–Guarantee reasoning paradigm has been applied in the last few years for the compositional verification of concurrent systems. Specifically, L^* has been used for learning the assumption, based on strings derived from counterexamples, which are given to it by a model-checker that attempts to verify the Assume–Guarantee rules. We suggest three optimizations to this paradigm. First, we derive from each counterexample multiple strings to L^*, rather than a single one as in previous approaches. This small improvement saves candidate queries and hence model-checking runs. Second, we observe that in existing instances of this paradigm, the learning algorithm is coupled weakly with the teacher. Thus, the learner completely ignores the details of the internal structure of the system and specification being verified, which are available already to the teacher. We suggest an optimization that uses this information in order to avoid many unnecessary membership queries (it reduces the number of such queries by more than an order of magnitude). Finally, we develop a method for minimizing the alphabet used by the assumption, which reduces the size of the assumption and the number of queries required to construct it. We present these three optimizations in the context of verifying trace containment for concurrent systems composed of finite state machines. We have implemented our approach in the ComFoRT tool, and experimented with real-life examples. Our results exhibit an average speedup of between 4 to 11 times, depending on the Assume–Guarantee rule used and the set of activated optimizations. This research was supported by the Predictable Assembly from Certifiable Components (PACC) initiative at the Software Engineering Institute, Pittsburgh.     

Lifelong exercise and stroke

OBJECTIVES: To examine the potential of lifelong patterns of increased physical activity to prevent stroke. DESIGN: Case-control study. SETTING: 11 general practices in west Birmingham. SUBJECTS: 125 men and women who had just had their first stroke and were aged 35-74 and 198 controls frequency matched for age and sex recruited over 24 months during 1988-90. Exclusion criteria were a previous history of stroke, mitral valvular heart disease combined with atrial fibrillation, primary or metastatic cerebral neoplasm, or coagulation disorder or myeloproliferative disease. MAIN OUTCOME MEASURES: Odds ratios for stroke related to lifetime history of exercise after 15 years of age. RESULTS: A history of vigorous exercise during the ages 15-25 appeared to protect from stroke: odds ratio adjusted for age and sex 0.33 (95% confidence interval 0.2 to 0.6). This effect was independent of other potential risk factors. Increasing years of participation in vigorous exercise between the ages of 15 and 55 produced an increasing protection from stroke (p < 0.001). In the 65 cases and 169 controls who were free of cardiac ischaemia, peripheral vascular disease, and poor health recent vigorous exercise and walking were protective against stroke: odds ratios of 0.41 (0.2 to 1.0) for recent vigorous exercise and 0.30 (0.1 to 0.7) for recent walking. CONCLUSIONS: Appreciable protection from stroke in later life is conferred by vigorous exercise in early adulthood. This increased level of physical activity should, if possible, be continued lifelong.     

Protein solubility and emulsifying capacity in frozen stored fish mince

Effect of initial freshness on the relationship between emulsifying capacity and protein solubility of frozen stored fish mince was investigated. The emulsifying capacity of proteins was significantly (P <0.05) affected by the initial freshness. A positive correlation (P <0.05) between protein solubility and the ability of the soluble proteins to emulsify and stabilise an emulsion was observed. Correlation between salt-soluble proteins and emulsifying capacity revealed that myofibrillar proteins (P <0.05) are the main proteins that decide the emulsion properties.     

Comparison of the effect of lansoprazole and omeprazole on intragastric acidity and gastroesophageal reflux in patients with gastroesophageal reflux disease

A prospective study of the pharmacokinetics of itraconazole solution was performed in 11 patients who underwent allogeneic BMT (day of BMT = day 0) after a conditioning regimen including total body irradiation (TBI). Itraconazole solution (400 mg once a day) was given 7 days before BMT and continued up to the end of neutropenia unless another antifungal treatment was necessary. Blood samples were collected before itraconazole intake (Cmin) and 4 h later (Cmax) every other day for assays of itraconazole (ITRA) and its active metabolite hydroxy-itraconazole (OH-ITRA). The mean values of Cmin ITRA and OH-ITRA, respectively, were 287 +/- 109 ng/ml and 629 +/- 227 ng/ml at day -1 and 378 +/- 147 ng/ml and 725 +/- 242 ng/ml at day +1. The maximum Cmin values were observed at day +3. Six patients at day -1 (54%) and 8 at day +1 (72%) had satisfactory residual plasma concentrations of at least 250 ng/ml of unchanged ITRA. From day +1 to day +9, eight patients discontinued the itraconazole treatment, five of them had satisfactory plasma residual concentrations at this time. This work shows a good bioavailability of itraconazole oral solution during the early phase after allogeneic BMT, but more data are needed for the late phases.     

Patterns of content, contextual, and working memory impairments in schizophrenia and nonamnesic alcoholism.

This study used tests of content memory (item recognition of words and abstract designs), context memory (order recognition of verbal and nonverbal items), and working memory (recognition at a short retention interval) to examine patterns of performance in 27 schizophrenic patients, 52 chronic alcoholic patients, and 66 healthy control participants. When performance was age- and IQ-adjusted the schizophrenia group was significantly impaired in item and order recognition of verbal and nonverbal material; the alcoholic group was impaired only in order recognition for both material types. Item- and order-recognition deficits in the schizophrenia group were greatest at the shortest retention intervals, a pattern previously observed in patients with Parkinson's disease, suggesting a prominence of a working memory deficit in schizophrenia. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Conductance studies on the interaction of hexamethylenetetramine with alkali halides in water and in formamide

The interaction of hexamethylenetetramine (HMT) with alkali halides in water and in formamide solution has been studied by employing conductance measurements. Conductance data of sodium and potassium halides in water and in formamide saturated by HMT at 50.0 ± 0.05° are reported at several temperatures within a range of 30 to 70°. Plots of ?log K against 1/T showed a break at the saturation temperature, where two straight lines intersect one another. Divergence of the pairs of straight lines in these ternary homogeneous systems has been found to be highly influenced by the structural properties of the electrolytes. The transitional behaviour in the conductance values is explained for the system, in terms of solute-solvent interactions involved in the electrolyte-solvent-nonelectrolyte systems.     

Compaction modelling of textile preforms for composite structures

This paper deals with modelling the compaction behaviour of dry fibre assemblies using an energy minimisation scheme. Compaction behaviour of textile preforms can significantly influence the resin permeability, fibre volume fraction and the geometry of individual tows. Tow geometry will in turn affect the elastic properties of the laminate, mode of damage initiation and progression. In this work, constitutive properties of yarns in bending and transverse compression were measured using Kawabata Evaluation System, and used for computing the potential energy stored in individual yarn segments within a preform. The compaction model has been experimentally verified for single and multi-layer 2D fabrics and 3D fabrics.