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151.
The budding yeast Saccharomyces cerevisiae has been a powerful model for the study of aging and has enabled significant contributions to our understanding of basic mechanisms of aging in eukaryotic cells. However, the laborious low‐throughput nature of conventional methods of performing aging assays limits the pace of discoveries in this field. Some of the technical challenges of conventional aging assay methods can be overcome by use of microfluidic systems coupled to time‐lapse microscopy. One of the major advantages is the ability of a microfluidic system to perform long‐term cell culture under well‐defined environmental conditions while tracking individual yeast. Here, recent advancements in microfluidic platforms for various yeast‐based studies including replicative lifespan assay, long‐term culture and imaging, gene expression, and cell signaling are discussed. In addition, emerging problems and limitations of current microfluidic approaches are examined and perspectives on the future development of this dynamic field are presented. 相似文献
152.
Moonsu Yoon Yanhao Dong Youngbin Yoo Seungjun Myeong Jaeseong Hwang Junhyeok Kim Seong‐Hyeon Choi Jaekyung Sung Seok Ju Kang Ju Li Jaephil Cho 《Advanced functional materials》2020,30(6)
A practical solution is presented to increase the stability of 4.45 V LiCoO2 via high‐temperature Ni doping, without adding any extra synthesis step or cost. How a putative uniform bulk doping with highly soluble elements can profoundly modify the surface chemistry and structural stability is identified from systematic chemical and microstructural analyses. This modification has an electronic origin, where surface‐oxygen‐loss induced Co reduction that favors the tetrahedral site and causes damaging spinel phase formation is replaced by Ni reduction that favors octahedral site and creates a better cation‐mixed structure. The findings of this study point to previously unspecified surface effects on the electrochemical performance of battery electrode materials hidden behind an extensively practiced bulk doping strategy. The new understanding of complex surface chemistry is expected to help develop higher‐energy‐density cathode materials for rechargeable batteries. 相似文献
153.
Jin Soo Kang Seoni Kim Dong Young Chung Yoon Jun Son Kyusik Jo Xiao Su Myeong Jae Lee Hwajoo Joo T. Alan Hatton Jeyong Yoon Yung‐Eun Sung 《Advanced functional materials》2020,30(9)
Given that a considerably large population suffers from shortage of water, there are numerous on‐going efforts to turn seawater into freshwater, and electrochemical desalination processes—particularly capacitive deionization (CDI)—have gained significant attention due to their high energy efficiency and reliable performance. Meanwhile, carbonaceous electrode materials, which are most commonly used in CDI systems, have poor long‐term stability due to unfavorable interactions with oxygen in saline water. Herein, rapid and vigorous inversion of surface charges in heteroatom‐doped carbon electrodes, which leads to a robust operation of CDI with high desalination capacity, is reported for the first time. By carbonization of coffee wastes, nitrogen‐ and sulfur‐codoped activated carbon with hierarchical micro/mesopores are prepared in an environmentally‐friendly manner, and this carbon results in a significantly higher inverted capacity than that of various activated carbon counterparts in long‐term CDI operations, without any sign of drop in performance. Investigations on the changes in physicochemical properties of the electrodes during the inversion disclose the favorable roles of nitrogen and sulfur dopants, which can be summarized as enlarging the difference between the surface charges of the two electrodes by chemical interactions with oxygen in the anode and carbon in the cathode. 相似文献
154.
Tae‐Woo Lee Taeyong Noh Hee‐Won Shin Ohyun Kwon Jong‐Jin Park Byoung‐Ki Choi Myeong‐Suk Kim Dong Woo Shin Yong‐Rok Kim 《Advanced functional materials》2009,19(10):1625-1630
Although significant progress has been made in the development of vacuum‐deposited small‐molecule organic light‐emitting diodes (OLEDs), one of the most desired research goals is still to produce flexible displays by low‐cost solution processing. The development of solution‐processed OLEDs based on small molecules could potentially be a good approach but no intensive studies on this topic have been conducted so far. To fabricate high‐performance devices based on solution‐processed small molecules, the underlying nature of the produced films and devices must be elucidated. Here, the distinctive characteristics of solution‐processed small‐molecule films and devices compared to their vacuum‐deposited counterparts are reported. Solution‐processed blue OLEDs show a very high luminous efficiency (of about 8.9 cd A–1) despite their simplified structure. A better hole‐blocking and electron‐transporting layer is essential for achieving high‐efficiency solution‐processed devices because the solution‐processed emitting layer gives the devices a better hole‐transporting capability and more electron traps than the vacuum‐deposited layer. It is found that the lower density of the solution‐processed films (compared to the vacuum‐deposited films) can be a major cause for the short lifetimes observed for the corresponding devices. 相似文献
155.
Yun-Jae Kim Man-Sik Myeong Kee-Bong Yoon 《International Journal of Pressure Vessels and Piping》2009,86(8):508-516
This paper provides effects of reinforcement shape and area on plastic limit loads of branch junctions under internal pressure and in-plane/out-of-plane bending, via detailed three-dimensional finite element limit analysis assuming elastic-perfectly plastic material behaviour. It is found that reinforcement is most effective when (in-plane/out-of-plane) bending is applied to the branch pipe. When bending is applied to the run pipe, reinforcement is less effective when bending is applied to the branch pipe. The reinforcement effect is the least effective for internal pressure. 相似文献
156.
Myeong Gi Lee Tae Hoon Ham Sang Hoon Song Dong Hwa Chung Won Byong Yoon 《Food science and biotechnology》2016,25(4):1073-1080
This study investigated the effects of high pressure homogenization (HPH) to replace a thermal pasteurization on the yeast cell inactivation and the volatile components of rice wine (RW). By applying HPH (172 MPa with 5 passes), the yeast count was reduced over 4 log cycles. The inactivation of yeast cells by HPH and a thermal processing, i.e. Holder pasteurization (HP), was quantitatively analyzed. In terms of the inactivation of yeast cells, the HPH at 172 MPa with 5 passes was equivalent to the HP at 65°C for 3 min. In RW, 34 volatile components were identified, including 8 alcohols, 18 esters, 2 acids, 1 carbonyls, 3 hydrocarbons, and 2 miscellaneous. Fruity volatiles were found more in HPH samples while components having fatty and oily characters were more detected in HP treated samples. 相似文献
157.
Eui-Chan Jeon Soojeong Myeong Jae-Whan Sa Jinsu Kim Jae-Hak Jeong 《Applied Energy》2010,87(1):205-210
Accurate estimation of greenhouse gas emissions is essential for developing an appropriate strategy to mitigate global warming. This study examined the characteristics of greenhouse gas emission from power plants, a major greenhouse gas source in Korea. The power plants examined use bituminous coal, anthracite, and sub-bituminous coal as fuel. The CO2 concentration from power plants was measured using GC–FID with methanizer. The amount of carbon, hydrogen, and calorific values in the input fuel was measured using an elemental analyzer and calorimeter. For fuel analysis, CO2 emission factors for anthracite, bituminous coal, and sub-bituminous coal were 108.9, 88.4, and 97.9 Mg/kJ, respectively. The emission factors developed in this study were compared with those for IPCC. The results showed that CO2 emission was 10.8% higher for anthracite, 5.5% lower for bituminous coal, and 1.9% higher for sub-bituminous coal than the IPCC figures. 相似文献
158.
Fermentation of Sargassum thunbergii by Kimchi‐Derived Lactobacillus sp. SH‐1 Attenuates LPS‐Stimulated Inflammatory Response Via Downregulation of JNK
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159.
160.
Sareer Ahmad Myeung Hoon Jo Muhammad Ikram Amjad Khan Myeong Ok Kim 《International journal of molecular sciences》2021,22(17)
The current study was undertaken to unveil the protective effects of Luteolin, a natural flavonoid, against amyloid-beta (Aβ1–42)-induced neuroinflammation, amyloidogenesis, and synaptic dysfunction in mice. For the development of an AD mouse model, amyloid-beta (Aβ1–42, 5 μL/5 min/mouse) oligomers were injected intracerebroventricularly (i.c.v.) into mice’s brain by using a stereotaxic frame. After that, the mice were treated with Luteolin for two weeks at a dose of 80 mg/kg/day. To monitor the biochemical changes, we conducted western blotting and immunofluorescence analysis. According to our findings, the infusion of amyloid-beta activated c-Jun N-terminal kinases (p-JNK), p38 mitogen-activated protein kinases, glial fibrillary acidic protein (GFAP), and ionized calcium adaptor molecule 1 (Iba-1) in the cortex and hippocampus of the experimental mice; these changes were significantly inhibited in Aβ1–42 + Luteolin-treated mice. Likewise, we also checked the expression of inflammatory markers, such as p-nuclear factor-kB p65 (p-NF-kB p65 (Ser536), tissue necrosis factor (TNF-α), and Interleukin1-β (IL-1β), in Aβ1–42-injected mice brain, which was attenuated in Aβ1–42 + Luteolin-treated mice brains. Further, we investigated the expression of pro- and anti-apoptotic cell death markers such as Bax, Bcl-2, Caspase-3, and Cox-2, which was significantly reduced in Aβ1–42 + Lut-treated mice brains compared to the brains of the Aβ-injected group. The results also indicated that with the administration of Aβ1–42, the expression levels of β-site amyloid precursor protein cleaving enzyme (BACE-1) and amyloid-beta (Aβ1–42) were significantly enhanced, while they were reduced in Aβ1–42 + Luteolin-treated mice. We also checked the expression of synaptic markers such as PSD-95 and SNAP-25, which was significantly enhanced in Aβ1–42 + Lut-treated mice. To unveil the underlying factors responsible for the protective effects of Luteolin against AD, we used a specific JNK inhibitor, which suggested that Luteolin reduced Aβ-associated neuroinflammation and neurodegeneration via inhibition of JNK. Collectively, our results indicate that Luteolin could serve as a novel therapeutic agent against AD-like pathological changes in mice. 相似文献