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Nadia Turton Neve Cufflin Mollie Dewsbury Olivia Fitzpatrick Rahida Islam Lowidka Linares Watler Cara McPartland Sophie Whitelaw Caitlin Connor Charlotte Morris Jason Fang Ollie Gartland Liv Holt Iain P. Hargreaves 《International journal of molecular sciences》2022,23(13)
Mitochondrial respiratory chain (MRC) disorders are a complex group of diseases whose diagnosis requires a multidisciplinary approach in which the biochemical investigations play an important role. Initial investigations include metabolite analysis in both blood and urine and the measurement of lactate, pyruvate and amino acid levels, as well as urine organic acids. Recently, hormone-like cytokines, such as fibroblast growth factor-21 (FGF-21), have also been used as a means of assessing evidence of MRC dysfunction, although work is still required to confirm their diagnostic utility and reliability. The assessment of evidence of oxidative stress may also be an important parameter to consider in the diagnosis of MRC function in view of its association with mitochondrial dysfunction. At present, due to the lack of reliable biomarkers available for assessing evidence of MRC dysfunction, the spectrophotometric determination of MRC enzyme activities in skeletal muscle or tissue from the disease-presenting organ is considered the ‘Gold Standard’ biochemical method to provide evidence of MRC dysfunction. The purpose of this review is to outline a number of biochemical methods that may provide diagnostic evidence of MRC dysfunction in patients. 相似文献
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Haq Nawaz Nadia Zafar Raheela Jabeen Adnan Amjad Mohibullah Shah Saba Munir 《European Journal of Lipid Science and Technology》2023,125(11):2300053
The repeated use of cooking oils and ghee for the deep frying of food materials may affect their nutritional quality. The present study evaluated the effect of repeated frying on the physicochemical characteristics and antiradical potential of canola oil and ghee. The oil and ghee were used for frying of fish and chicken for 2, 4, 6, 8, and 10 frying cycles followed by the analysis of physicochemical, oxidative stress, and antiradical parameters. Regression analysis of the data showed a frying cycle-dependent significant linear increase in saponification (R2 = 0.9507–0.9748), peroxide and acid values (R2 = 0.956–0.9915), and malondialdehyde (MDA) production (R2 = 0.9058–0.9557) of canola oil and ghee subjected to fish and chicken frying but exponential increase in saponification value (R2 = 0.9778) and MDA production (R2 = 0.7407) of canola oil and ghee used for fish frying. The increase in the number of frying cycles linearly decreased the iodine value (R2 = 0.9781–0.9924), and 1, 1-diphenyl-2-picrylhydrazyl, hydroxyl, and 2, 2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) radical scavenging potential (R2 = 0.9089–0.9979) of canola oil and ghee. Repeated frying in cooking oil and ghee increases oxidative stress and decreases their physicochemical and antioxidant qualities. Canola oil was comparatively more oxidative resistant than canola ghee. The regression equations derived from regression analysis will guide researchers to conduct similar types of univariate studies. 相似文献
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Juzheng Zhang Jianmin Zheng Nadia Magnenat‐Thalmann 《Computer Animation and Virtual Worlds》2015,26(3-4):237-245
How to endow the virtual characters with personalized behavior patterns remains a challenging problem. Instead of heuristically designing behaviors for certain personalities, this paper bridges the gap between personalities and behaviors using a medium concept, mood, to make the behaviors of the characters consistent enough to convey their personalities, while flexible enough to make appropriate response in various situations. We propose a personality‐characterized mood dynamics model, in which the emotion weights are computed as a solution of a convex optimization problem that is constructed to make the overall mood approaches the personality after sufficient interactions. The convergence of the algorithm is demonstrated by numerical simulations. The implementation of the personality‐characterized mood dynamics model enables an emotion‐oriented virtual human, Sophie, to show personalized behaviors in the emotional interactions with users. Copyright © 2015 John Wiley & Sons, Ltd. 相似文献
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