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排序方式: 共有886条查询结果,搜索用时 281 毫秒
51.
Ito A Shinkai M Hakamada K Honda H Kobayashi T 《Journal of Bioscience and Bioengineering》2001,92(6):598-601
We demonstrated the effectiveness of radiation-inducible expression of the TNF-alpha gene for cancer therapy in vitro. The TNF-alpha gene under the control of the stress-inducible promoter, gadd 153, was introduced into the human glioma cell line, U251-SP. Without cobalt-60 gamma irradiation, no cytotoxicity against the transfected cells was observed. When the transfected cells were irradiated with 10 or 20 gray (Gy), the gadd 153 promoter was highly induced and the expression level of TNF-alpha increased. Five days after the irradiation, the TNF-alpha productions of each cell irradiated with 10 and 20 Gy were 30 and 100 times higher than the basal level, respectively. The cytotoxicities against the transfected cells 5 d after irradiation with 10 and 20 Gy were 79% or 91%, respectively, which are much higher than those against the nontransfected cells that were irradiated at the same dose (43% and 78%, respectively). These results demonstrate that the gadd 153-TNF-alpha system may be an effective tool for radiosurgery of malignant brain tumors. 相似文献
52.
Ito T Kikuta H Nagamori E Honda H Ogino H Ishikawa H Kobayashi T 《Journal of Bioscience and Bioengineering》2001,91(3):245-250
Efficient lipase production by two-step fed-batch culture of an organic solvent-tolerant bacterium, Pseudomonas aeruginosa LST-03, was investigated. When FB synthetic medium was used in flask culture, no lipase activity was detected, whereas lipase was produced at 2.3 I.U./ml in C2 complex medium. However, lipase production was induced in FB medium when a fatty acid was added to the culture broth in the stationary phase. Among fatty acids tested, long chain saturated fatty acids, such as C18 (stearic acid) and C20 (arachidic acid), were found to function as effective inducers for the production of lipase, giving an activity level almost the same as that obtained in C2 medium in flask culture. Two-step lipase production, comprised of a growth phase in fed-batch mode and a production phase in which lipase was induced by the addition of 5% (v/v) stearic acid, was carried out in a jar-fermentor. In the growth phase, the maximum cell concentration at 16 h was only 20 in terms of the optical density at 660 nm (OD660), and a low level of lipase production (8 I.U./ml) was obtained after 167 h. This was considered to be due to the exhaustion of several medium components brought about by the use of an unsuitable medium or feeding solution. After analyzing the contents of the compounds in the culture broth by inductively coupled plasma spectrometry for metal ions and HPLC for anions, a modified FB medium was designed. When this modified FB medium was used in two-step fed-batch culture, the maximum cell concentration reached an OD660 of 55 (30.2 g-dry cells/l) at 16.5 h, and lipase was produced at 96 I.U./ml after 35 h, which is approximately 40 times higher than the production level obtained in flask culture using C2 medium. 相似文献
53.
54.
A Low-Power Low-Voltage 10-bit 100-MSample/s Pipeline A/D Converter Using Capacitance Coupling Techniques 总被引:2,自引:0,他引:2
This paper presents a low-power low-voltage 10-bit 100-MSample/s pipeline analog-to-digital converter (ADC) using capacitance coupling techniques. A capacitance coupling sample-and-hold stage achieves high SFDR with 1.0-V supply voltage at a high sampling rate. A capacitance coupling folded-cascode amplifier effectively saves the power consumption of the gain stages of the ADC in a 90-nm digital CMOS technology. The SNDR and the SFDR are 55.3 dB and 71.5 dB, respectively, and the power consumption is 33 mW 相似文献
55.
M. Matsuoka T. Hayasaka Y. Fukushima Y. Honda 《International journal of remote sensing》2013,34(2):221-248
Land cover is classified over East Asia using 250‐m Moderate Resolution Imaging Spectroradiometer (MODIS) land surface reflectance, MODIS snow cover and Operational Linescan System (OLS) human settlement data. The classification method includes a decision tree classification scheme that considers 11 kinds of land surface features derived from the OLS product and the time series of two MODIS products in 2000. The decision tree was defined manually based on the experiment because of insufficient training data, ease of tuning by visual interpretation, and extensibility to further research. The resulting classification is compared to three kinds of reference data, i.e. MODIS land cover product, Chinese digital land cover map, and Chinese census. The land cover classification can be input into a hydrological model applied to the Yellow River in China. 相似文献
56.
Daniel Kassahun Nitin K. Tripathi Kiyoshi Honda 《International journal of remote sensing》2013,34(19):4213-4225
Satellite‐based monitoring is an indispensable tool to guide soil‐specific crop management. However, it has attained little success in the estimation of soil nutrients due to the limitations incurred from inherent spectral characteristics. In this study, spectral band cloning (SBC) is developed and proposed to augment the soil nutrient predictive capabilities of broadband satellite data. Fine‐spectral channels of spectrometers were synchronized with coarse resolution of IRS satellite data to generate nutrient‐sensitive cloned IRS bands. Soil samples, collected at the time of satellite image acquisition in Lop Buri, Thailand, were analyzed both spectrally and chemically, viz., soil organic matter (OM), phosphorus, potassium and iron. The resulting SBC‐based models showed acceptable correlations, which otherwise were unattainable from raw IRS bands through prevailing models. Accuracy and validation measures showed good agreements between the measured and estimated nutrient surfaces. It is concluded that the SBC is a promising method of quantitative soil nutrient mapping, and could further be used for identification and mapping of other indiscernible biophysical parameters. 相似文献
57.
Sujittra Charoenhirunyingyos Kiyoshi Honda Daroonwan Kamthonkiat Amor V. M. Ines 《International journal of remote sensing》2013,34(23):8033-8051
Leaf area index (LAI) and actual evapotranspiration (ETa) from satellite observations were used to estimate simultaneously the soil hydraulic parameters of four soil layers down to 60 cm depth using the combined soil water atmosphere plant and genetic algorithm (SWAP–GA) model. This inverse model assimilates the remotely sensed LAI and/or ETa by searching for the most appropriate sets of soil hydraulic parameters that could minimize the difference between the observed and simulated LAI (LAIsim) or simulated ETa (ETasim). The simulated soil moisture estimates derived from soil hydraulic parameters were validated using values obtained from soil moisture sensors installed in the field. Results showed that the soil hydraulic parameters derived from LAI alone yielded good estimations of soil moisture at 3 cm depth; LAI and ETa in combination at 12 cm depth, and ETa alone at 28 cm depth. There appeared to be no match with measurement at 60 cm depth. Additional information would therefore be needed to better estimate soil hydraulic parameters at greater depths. Despite this inability of satellite data alone to provide reliable estimates of soil moisture at the lowest depth, derivation of soil hydraulic parameters using remote sensing methods remains a promising area for research with significant application potential. This is especially the case in areas of water management for agriculture and in forecasting of floods or drought on the regional scale. 相似文献
58.
Volcanic disasters can cause severe loss of human life and damage to property. The main damage is caused during an eruption and from subsequent erosion of deposited materials. Heavy rainfall in volcanic areas erodes volcanic deposits, mainly pyroclastic flows and ash fall deposits, which flow as lahar to the foothill of the mountain and cause drastic damage to economically important areas. This post-eruption disaster becomes complex due to the occurrence of stream captures and watershed breakouts that lead to devastating lahars. Continuous monitoring of such geomorphic and hydrologic changes is necessary to cope with changing hazard conditions. Therefore it is important to update the watershed boundaries in order to study current hazard conditions and develop mitigation plans for future disasters. Changes of geomorphic and watershed boundary have occurred in the Mayon Volcano in the Philippines mainly as a result of a major volcanic eruption of 1993, due to which mitigation structures were constructed and modified in the low lying areas. In this study interferometry was used to develop DEM from SAR data to delineate watershed boundaries. New lava flows, pyroclastic flows and lahar deposits in the watersheds were mapped using elevation changes, coherence and intensity derived from the SAR images. Updating geomorphic features of the watersheds and their boundaries using SAR provides a new weather independent alternative technique for monitoring the effect of volcanic activity. 相似文献
59.
Toshio Honda 《Sequential Analysis》2013,32(1):73-89
Let X1, X2... be i.i.d. observations with mean μ and variance σ2, and suppose that given a sample of size n one wishes to estimate μ by Xn, subject to the loss function Ln= A2(Xn-μ)2+n. When σ2 is unknown, the optimal sample size n0 for minimizing the risk Rn = E{Ln} cannotbe found. To resolve this problem, Robbins (1959) proposed a sequential procedure whenXi is normally distributed, and then the asymptotic properties have been extensively studied. In this paper, we apply Hall's three stage procedure to the problem without specifying the distribution of Xi and derive the second order asymptotic expansions of the expected sample size and the risk. 相似文献
60.
Toshio Honda 《Sequential Analysis》2013,32(3-4):239-251
Suppose that {Xn} is a strongly mixing process with unknow marginal density f(x) and that we estimate f(x) by a kernel estimator [fcirc]n(x|hn)and want to achive the MISE no larger than some preassigned postive number w. However,the appropriate sample size n*depends on a functional of the unknow density function. Therefore some sequential procedure is required and we adopt a fully sequential procedure. In this paper we investigate the asymptotic properties of the procedure and show that the producure is asymptotically efficient in a certain sense as w→0. The results are almost the same in the i.i.d. setting. our result extend a class of models to which the methodology can be applied. For example economic variable,experiments on a single subject in which obervation are not indepent, and so on. 相似文献