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81.
Nicholas Martinus D. Crawford D. Sinclair Colin A. Vincent 《Electrochimica acta》1977,22(10):1183-1187
A number of authors have recently interpreted the relative viscosities of electrolytic solutions in terms of an extended Jones—Dole equation containing a term Dc2: The B-coefficients resulting from such analyses are not directly comparable with those arising from the simple Jones—Dole expression because of the high negative correlation between the B and D terms. There is therefore some difficulty in relating results from both types of analysis. In this paper a number of data sets are examined statistically with a view to establishing a standard procedure for obtaining estimates of the B-coefficients which relate exclusively to the linear term and are not influenced by the effect of higher terms. Two methods which recognize the high correlation of B and D terms are suggested. 相似文献
82.
83.
Gillian M. Keith Kumaravinothan Sarma Neil McN. Alford Derek C. Sinclair 《Journal of Electroceramics》2004,13(1-3):305-309
Hexagonal BaTiO3 materials have been stabilised at room temperature according to the formula BaTi0.95M0.05 O3– where M = Mn, Fe, Co and Ni. Dense ceramics (> 96% of the theoretical X-ray density) were sintered at 1450C in flowing O2 gas from calcined powders prepared by the mixed oxide route at 1300C. All samples were single-phase and the bulk conductivity, b, measured by Impedance Spectroscopy and Q.f measured by microwave dielectric resonance methods showed a strong dependence on the type of dopant. b at 300C was 10–7, 10–5.5, 10–5.5 and 10–4 Scm–1 for M = Mn, Fe, Ni and Co, respectively and Q.f at 5 GHz was 7790, 6670, 2442 and 1291 GHz, for M = Mn, Fe, Ni and Co, respectively. The correlation between b and Q.f is attributed to the presence of oxygen vacancies and/or mixed valency of the dopant ions. 相似文献
84.
85.
We measure with a gated intensified CCD camera the cross-polarized backscattered light from a linearly polarized laser beam penetrating a cloud made of spherical particles. In accordance with previously published results we observe a clear azimuthal pattern in the recorded images. We show that the pattern is symmetrical, that it originates from second-order scattering, and that higher-order scattering causes blurring that increases with optical depth. We also find that the contrast in the symmetrical features can be related to measurement of the optical depth. Moreover, when the blurring contributions are identified and subtracted, the resulting pattern provides a pure second-order scattering measurement that can be used for retrieval of droplet size. 相似文献
86.
87.
Smith CJ Wilson ID Abou-Shakra F Payne R Parry TC Sinclair P Roberts DW 《Analytical chemistry》2003,75(6):1463-1469
The use of high performance liquid chromatography coupled with inductively coupled plasma mass spectrometry (HPLC-ICPMS) as means for the quantitative determination of ZD0473, a platinum anticancer drug, and its related biologically active "aqua" compounds in biofluid samples is described. The performance of the resulting HPLC-ICPMS method was compared with that of a conventional HPLC-triple quadrupole mass spectrometer-based (HPLC-MS/MS) system for properties such as limit of detection, linearity, and reproducibility using spiked samples. The methods were then applied to the determination of plasma ultrafitrate concentrations of ZD0473 in dog plasma samples obtained following intravenous and oral administration at 0.5 and 6 mg/kg, respectively. These experiments showed that both methods were capable of providing accurate and precise results but that the HPLC-ICPMS method had advantages of extended linear range and superior sensitivity, providing a limit of quantification of 0.1 ng/mL for ZD0473, as compared to 5 ng/mL using the current HPLC-MS/MS method. In addition, by using a single combined HPLC-ICPMS/MS/MS system, it was possible to determine the relative MS/MS response of the aqua compounds for the first time. 相似文献
88.
Glidle A Yasukawa T Hadyoon CS Anicet N Matsue T Nomura M Cooper JM 《Analytical chemistry》2003,75(11):2559-2570
We describe a method, based on X-ray photoelectron spectroscopy (XPS) measurements, to assess the extent of protein adsorption or binding on a variety of different muTAS and biosensor interfaces. Underpinning this method is the labeling of protein molecules with either iodine- or bromine-containing motifs by using protocols previously developed for radiotracer studies. Using this method, we have examined the adsorption and binding properties of a variety of modified electrodeposited polymer interfaces as well as other materials used in muTAS device fabrication. Using polymer interfaces modified with poly(propylene glycol) (PPG) chains, our results indicate that a chain of at least approximately 30 monomer units is required to inhibit nonspecific adsorption from concentrated protein solutions. The XPS methodology was also used to probe specific binding of avidins and enzyme conjugates thereof to biotinylated and mixed biotin/PPG-modified polymer interfaces. In one example, using competitive binding, it was established that the mode of binding of a peroxidase-streptavidin conjugate to a biotinylated modified polymer interface was primarily via the streptavidin moiety (as opposed to nonspecific binding via the enzyme conjugate). XPS evaluation of nonspecific and specific peroxidase-streptavidin immobilization on various functionalized polymers has guided the design and fabrication of functionalized interdigitated electrodes in a biosensing muTAS device. Subsequent characterization of this device using scanning electrochemical microscopy (SECM) corroborated the adsorption and binding previously inferred from XPS measurements on macroscale electrodes. 相似文献
89.
Ravet N Abouimrane A Armand M 《Nature materials》2003,2(11):702; author reply 702-702; author reply 703
90.
Advances in biotechnology in recent decades have revolutionized our understanding of early mammalian development and promise to provide ever more finely tuned and precisely targeted techniques for genetic enhancement of domestic animal species. In demonstrating what is both technically and biologically possible, not only in mice but also in larger animal species, research has provided hope that previously intractable diseases and genetic defects can be successfully combated. Crucial to this research is the ability to culture oocytes, embryos and somatic cells in vitro and to sustain their development without inducing adverse short- or long-term consequences. There is a need to refine current culture strategies in farm animal species to avoid jeopardizing their dependent technologies. A key to resolving current limitations of culture strategies is to identify, acknowledge and then address those features of in vitro culture that compromise early regulation of mammalian development. The aim of this review is to appraise critically in vitro embryo and somatic cell production strategies in the context of their impact on developmental competence and normality at embryonic, fetal and later stages. In addition, effects of physically manipulating embryos and cells, most notably via nuclear and gene transfer technologies, are considered with a view to identifying how detrimental consequences can be avoided. 相似文献