Atomic force microscopy of the morphology and mechanical behaviour of barnacle cyprid footprint proteins at the nanoscale

Barnacles are a major biofouler of man-made underwater structures. Prior to settlement, cypris larvae explore surfaces by reversible attachment effected by a ‘temporary adhesive’. During this exploratory behaviour, cyprids deposit proteinaceous ‘footprints’ of a putatively adhesive material. In this study, footprints deposited by Balanus amphitrite cyprids were probed by atomic force microscopy (AFM) in artificial sea water (ASW) on silane-modified glass surfaces. AFM images obtained in air yielded better resolution than in ASW and revealed the fibrillar nature of the secretion, suggesting that the deposits were composed of single proteinaceous nanofibrils, or bundles of fibrils. The force curves generated in pull-off force experiments in sea water consisted of regions of gradually increasing force, separated by sharp drops in extension force manifesting a characteristic saw-tooth appearance. Following the relaxation of fibrils stretched to high strains, force–distance curves in reverse stretching experiments could be described by the entropic elasticity model of a polymer chain. When subjected to relaxation exceeding 500 ms, extended footprint proteins refolded, and again showed saw-tooth unfolding peaks in subsequent force cycles. Observed rupture and hysteresis behaviour were explained by the ‘sacrificial bond’ model. Longer durations of relaxation (>5 s) allowed more sacrificial bond reformation and contributed to enhanced energy dissipation (higher toughness). The persistence length for the protein chains (L_P) was obtained. At high elongation, following repeated stretching up to increasing upper strain limits, footprint proteins detached at total stretched length of 10 µm.     

A 2D approximation of standard electrical steel losses and permeability

A first design approach for an electrical machine need not be done with the efforts of detailed magnetic field considerations. Often a more pragmatic way already fulfills the desired accuracy. Performing fast by hand calculations depends on the data that is available; for e.g., the lamination geometry or material. This article provides a table for different electrical steels that can be used in the analytical calculation of magnetic circuits. In comparison with existing publications this article shows two-dimensional functions for the specific iron losses and the relative permeability. Both magnetic flux density and frequency are input variables.     

Benefit evaluation for off‐site production in construction

Evaluating to what extent a component or building system should be produced off‐site is inadequate within the industry. The potential benefits of off‐site production (OSP) are commonly cited when justifying an OSP approach, yet holistic and methodical assessments of the applicability and overall benefit of these solutions, to a particular project, have been found to be deficient. Common methods of evaluation simply take material, labour and transportation costs into account when comparing various options, often disregarding other cost‐related items such as site facilities, crane use and rectification of works. These cost factors are usually buried within the nebulous preliminaries figure, with little reference to the building approach taken. Further, softer issues such as health and safety, effects on management and process benefits are either implicit or disregarded within these comparison exercises. Yet it is demonstrated that these issues are some of the most significant benefits of OSP. A series of case studies demonstrated that evaluation focus is almost solely on direct material and labour costs of components, without explicit regard for the wider cost or soft issue implications of OSP on a project. The paper argues that until evaluation is more holistic and value‐based rather than cost‐based, OSP uptake in construction will be slow.     

Motivation,commitment and the use of incentives in partnerships and alliances

The use of incentives in partnering and alliancing has been seen as an important way of reinforcing collaboration in the short term and helping to build trust between clients and contractors in the long term. Yet only rarely has the impact of incentives on such relationships been discussed, let alone subjected to systematic investigation. This is despite a wealth of theory and research which brings into question the use of incentives and reinforcers as ways of generating motivation and commitment. Drawing upon this theoretical knowledge base and using evidence from a number of case studies of partnerships and alliances, this paper demonstrates how a number of important cognitive and social dimensions affect the use and impact of incentives, sometimes in ways contrary to those intended. The conclusion drawn is that there are important limitations to the use of incentives as means of reinforcing collaboration and developing commitment and trust, and that this raises questions more generally about the assumptions that underlie many of the practical ‘tools and techniques’ commonly associated with partnering and alliancing.     

Thermal comfort in outdoor urban spaces: understanding the human parameter

The research being undertaken seeks to achieve a better understanding of the richness of microclimatic characteristics in outdoor urban spaces, and the comfort implications for the people using them. The underlying hypothesis is that these conditions influence people’s behaviour and usage of outdoor spaces. The initial results demonstrate that a purely physiological approach is inadequate in characterising comfort conditions outdoors, and an understanding of the dynamic human parameter is necessary in designing spaces for public use. The thermal environment is indeed of prime importance influencing people’s use of these spaces, but psychological adaptation (available choice, environmental stimulation, thermal history, memory effect, expectations) is also of great importance in such spaces that present few constraints.     

An international quality assurance scheme for the quantitation of daidzein and genistein in food,urine and plasma

This quality assurance (proficiency testing) scheme was commissioned to enable the Food Standards Agency (FSA) to determine the quality of analytical results submitted by researchers measuring the concentrations of phytoestrogens in foods and biological fluids in FSA-funded research projects, and also, to demonstrate that FSA-funded laboratories are producing consistent and precise results. Non-FSA-funded laboratories from around the world were also invited to join in the scheme to increase the number participants. A secondary objective was to highlight the most successful methodologies used to analyse phytoestrogens.     

Transfer in artificial grammar learning: A reevaluation.

This article covers methodological and theoretical issues in artificial grammar learning. Arguments that such tasks are mediated by abstract knowledge (e.g., A. S. Reber, see record 1991-00330-001) are based primarily on evidence from transfer experiments, where the surface vocabulary is changed between learning and test items. Because of a number of methodological concerns, the small magnitudes of artificial grammar learning effects generally are difficult to interpret. Possible solutions are offered here. Furthermore, even reliable transfer effects imply neither that subjects have acquired abstract knowledge of the underlying grammar nor that they are performing a process of abstract analogy from memorized whole exemplars. Models that learn only surface fragments of the training stimuli and perform abstraction at test rather than during learning are wholly consistent with transfer phenomena. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Role of the alpha-glucanase Agn2p in ascus-wall endolysis following sporulation in fission yeast

During sporulation in the ascomyceteous fungus Schizosaccharomyces pombe, diploid cells undergo differentiation into asci containing four haploid ascospores, which are highly resistant to environmental stresses. Although the morphogenetic processes involved in ascospore formation have been studied extensively, little is known about the molecular mechanism that ensures the release of mature ascospores from the ascus, allowing their dispersal into the environment. Recently, we identified Agn2p as the paralogue of the characterized endo-(1,3)-alpha-glucanase Agn1p, and observed that asci deleted for agn2 are defective in ascospore dispersal. Here, we focus on the cellular and biochemical functions of Agn2p. By placing agn2 under the control of an inducible promoter, we show that expression of agn2 is required for the efficient release of ascospores from their asci. Furthermore, we characterize the enzyme activity of purified recombinant Agn2p and show that Agn2p, like Agn1p, is an endo-(1,3)-alpha-glucanase that produces predominantly (1,3)-alpha-glucan pentasaccharides. Finally, we demonstrate that exogenous addition of purified Agn2p liberated the ascospores from asci deleted for agn2. We propose that Agn2p participates in the endolysis of the ascus wall by hydrolysing its (1,3)-alpha-glucan, thereby assisting in the release of ascospores.