Constructing ensembles of classifiers using supervised projection methods based on misclassified instances

In this paper, we propose an approach for ensemble construction based on the use of supervised projections, both linear and non-linear, to achieve both accuracy and diversity of individual classifiers. The proposed approach uses the philosophy of boosting, putting more effort on difficult instances, but instead of learning the classifier on a biased distribution of the training set, it uses misclassified instances to find a supervised projection that favors their correct classification. We show that supervised projection algorithms can be used for this task. We try several known supervised projections, both linear and non-linear, in order to test their ability in the present framework. Additionally, the method is further improved introducing concepts from oversampling for imbalance datasets. The introduced method counteracts the negative effect of a low number of instances for constructing the supervised projections.The method is compared with AdaBoost showing an improved performance on a large set of 45 problems from the UCI Machine Learning Repository. Also, the method shows better robustness in presence of noise with respect to AdaBoost.     

Identification of unbalance forces by metaheuristic search algorithms

This paper discusses the identification of parameters in rotary systems, namely, the unbalance magnitude, phase and position in the rotor system. These parameters can be identified using the measured orbits in the hydrodynamic bearings. The oil film forces are evaluated in the different positions of the orbit of the journal and are applied to the model of the shaft. The model, integrated in time domain, allows with an assumed unbalance, to simulate the orbits. The objective function is basically the difference between measured and simulated orbits, and its minimum corresponds to the identified unbalance amount, phase and position along the shaft. With respect to traditional model based identification procedures, this approach using oil film forces instead of oil film linearized stiffness and damping coefficients, and unfiltered orbits instead of 1X vibration components is suitable to deal with non-linear behaviour of the system.     

p72: a human nuclear DEAD box protein highly related to p68

P72, a novel human member of the DEAD box family of putative RNA-dependent ATPases and ATP-dependent RNA helicases was isolated from a HeLa cDNA library. The predicted amino acid sequence of p72 is highly homologous to that of the prototypic DEAD box protein p68. In addition to the conserved core domains characteristic of DEAD box proteins, p72 contains several N-terminal RGG RNA-binding domains and a serine/glycine rich C-terminus likely involved in mediating protein-protein interactions. A p72-specific probe detects two mRNAs of approximately 5300 and 9300 bases which, although ubiquitously expressed, show variability in their expression levels in different tissues. Purified recombinant p72 exhibits ATPase activity in the presence of a range of RNA moieties. Immunocytochemical studies of p68 and p72 show that these proteins localise to similar locations in the nucleus of HeLa cells, suggesting their involvement in a nuclear process.     

Creep behaviour of butt-welded joints

A theoretical investigation has been made of the strains accumulating in the steady state creep of butt-welded joints. This was done by using a refined plate theory-Cosserat plate theory. Appropriate constitutive equations were developed for the theory which correspond to standard uniaxial and three-dimensional equations. In particular, a choice of Norton's Law, $\text{? = Aσ}{}^{\mathrm{n}}$ was made. Then, the plane strain problem of a plate containing a weld of different mechanical and creep properties was solved for the case of tensile loading. Results were obtained both numerically and analytically for a wide range of parameter values: for different ratios of the creep strengths, $\text{A}$, of the parent and weld metals, for values of the index, $\text{n}$, (including the limit $\text{n}$ → ∞) and for values of the length, $\text{l}$, of the weld (including the limit $\text{l}$ → 0).     

The kinetics of the reaction between copper(II) and some ortho-hydroxyoximes

The kinetics of the reaction between copper(II) and salicylaldoxime were studied in various ethanol-water mixtures. The rate of reaction was found to be first order in both copper(II) and the neutral ligand. Deviations from this behaviour at low pH can be accounted for if allowance is made for the reverse reaction and for protonation of the ligand. A mechanism that involves the rate-determining formation of the mono-complex has been found to describe the kinetic data with the rate constant in 80 per cent ethanol being 3.7 × 10⁴ mol^?4 ls^?1 at 11°C. The kinetic data have been used in a preliminary assessment of the location of the reaction in the extraction of copper(II) by hydroxime solvents.     

The expression of steroidogenic acute regulatory protein (StAR) in bovine adrenocortical cells

StAR protein may facilitate rapid transfer of cholesterol from the outer to the inner mitochondrial membrane, the site at which cholesterol is converted to pregnenolone by the cholesterol side chain cleavage complex. We have studied the effect of ACTH treatment on StAR mRNA and protein levels in bovine adrenocortical cells in primary culture. Cells were initially cultured for 3 days after isolation, and then treated with ACTH (10(-8) M) for various times up to 24 hours. Northern analysis of total BAC mRNA, using a [alpha32P]-labelled cDNA probe encoding a 5' region of bovine StAR mRNA, revealed two principal hybridising species of 1.6 and 3.0 kb. Western immunoblot analysis revealed a principal band at 30 kDa. Levels of both StAR mRNA and protein showed an increase at 1 hour, reached a maximum at around 6 hours and declined to basal levels at 24 hours. Cortisol secretion (measured by RIA) showed a similar change over the same period. From these results it appears that StAR mRNA and protein levels in BAC are acutely regulated in concert with ACTH-stimulated cortisol secretion.     

Reduced stretch-reflex sensitivity after exhausting stretch-shortening cycle exercise

The stretch-shortening cycle (SSC) is an effective and natural form of muscle function but, when repeated with sufficient intensity or duration, it may lead to muscle damage and functional defects. A reduced tolerance to impact has been reported, which may be partly attributed to a reduced stretch-reflex potentiation. The aim of the present study was to examine the influence of SSC-induced metabolic fatigue and muscle damage on the efficacy of stretch reflexes, as judged by the electromyograph (EMG) response of two shank muscles (lateral gastrocnemius LG, soleus SOL) to controlled ramp stretches. These EMG responses were recorded before and immediately after exhausting SSC-type leg exercise and 2 h, 2 days and 4 days later. Serum concentrations of creatine kinase ([CK]), myoglobin and lactate were measured repetitively along the protocol. Two maximal vertical drop jumps and counter-movement jumps were performed after each reflex test. The exhausting SSC-type exercise induced an immediate reduction (P < 0.05) with a delayed short-term recovery of the LG peak-to-peak reflex amplitude. This was not accompanied by significant changes in the reflex latency. The drop jump performance remained slightly but significantly reduced (P < 0.05) until the 2nd day postexercise. Peak [CK] appeared for all the subjects on the 2nd day, suggesting the presence of muscle damage. The increase in [CK] between the 2nd h and the 2nd day postexercise was found to be negatively related (P < 0.001) to the relative changes in the drop jump height. Furthermore, a significant relationship (P < 0.05) was found between recovery of the stretch reflex in LG and the decrease of [CK] between the 2nd and the 4th day. These findings support the hypothesis of a reduced stretch-reflex sensitivity. While the exact mechanisms of the reflex inhibition remain unclear, it is emphasized that the delayed recovery of the reflex sensitivity could have resulted from the progressive inflammation that develops in cases of muscle damage.     

A low-power 128-tap digital adaptive equalizer for broadband modems

This paper describes a modem receiver chip containing two 64-tap adaptive finite impulse response (FIR) filters configured in parallel as in-phase and quadrature-phase filters. Each filter has a span of 16 symbols and can be configured for T/2, T/3, or T/4 fractional spacing. A zero-latency pipeline technique is used that allows adaptive filters of arbitrary length without degrading the speed. Power is saved at the algorithmic, architectural, and circuit levels. The chip has support for dynamically tuning coefficient precision, updating rates and filter lengths to reduce power consumption. The chip was fabricated in 0.5-μm CMOS technology and consumes 535 mW of power when operating at 50 MHz with 128 taps, T/4 spacing, and symbol-rate power-of-two LMS updating. This can be further reduced to 280 mW using dynamic power reduction techniques. The power in the FIR filter is 162 mW with maximum precision converged coefficients which corresponds to 5.1 mW per multiply-accumulate operation     

Dendritic cells stimulate the expansion of bcr-abl specific CD8+ T cells with cytotoxic activity against leukemic cells from patients with chronic myeloid leukemia

The role of T lymphocytes in the control of chronic myeloid leukemia (CML) after bone marrow transplantations has been clearly shown. This effect closely correlates with graft-versus-host disease (GVHD). A specific graft-versus-leukemia (GVL) effect separate from GVHD has been postulated but has been difficult to show. One possible target for specific GVL activity is the bcr-abl fusion protein characteristic of CML. We have investigated the use of normal peptide-pulsed dendritic cells for the generation of cytotoxic, bcr-abl-specific T cells from normal donors. T cells (CD3+, CD8+, TCR alpha beta+, and NK receptor-negative) generated from a normal donor (HLA A24, B52, B59, Cw1) after stimulation with autologous dendritic cells, primed with a 16 mer peptide spanning the b3a2 breakpoint of bcr-abl, lysed CML cells from the peripheral blood of seven patients with CML with the b3a2 breakpoint. CML cells from four patients with only the b2a2 breakpoint were not lysed. Phytohemagglutinin (PHA) blasts derived from peripheral blood of patients with CML were not lysed, suggesting that cytotoxicity was not due to alloreactivity. Blocking experiments with anti-HLA-A,B,C indicated that cytotoxicity was dependent on recognition of major histocompatibility complex (MHC) class I molecules, although cytotoxicity was not MHC-restricted because not all patients shared HLA types with the T-cell donor. Specificity for bcr-abl and absence of alloreactivity was confirmed by the presence of lytic activity against autologous and allogeneic class I HLA-A matched monocytes pulsed with the 16 mer bcr-abl fusion peptide, but not against unpulsed monocytes or monocytes pulsed with other peptides. These results show that bcr-abl-specific T cells with marked cytotoxic activity against CML cells can be generated and amplified from normal donor peripheral blood. Recognition of HLA molecules is essential for cytotoxicity but strict HLA identity is not required.     

Suppression of arousal by progesterone in fetal sheep

The high rate of progesterone synthesis by the placenta in late gestation exposes the ovine fetus to high concentrations of progesterone and its metabolites that may affect activity of the fetal brain. The aim of this study was to determine the effect of inhibiting maternal progesterone synthesis on sleep-wake activity in fetal sheep. Fetal and maternal vascular catheters, a fetal tracheal catheter, and electrodes for recording fetal electrocortical (ECoG), electro-ocular (EOG) and nuchal muscle electromyographic (EMG) activity were implanted. At 128-131 days gestation, progesterone production was inhibited by an injection of trilostane (50 mg), a 3beta-hydroxysteroid dehydrogenase inhibitor. Vehicle solution or progesterone (3 mg h(-1)) was then infused into the ewe between 6 and 12 h after the trilostane treatment. Maternal progesterone concentrations were significantly reduced from 1-24 h after trilostane treatment (P < 0.05) when followed by vehicle infusion. Fetal breathing movements (FBM), EOG, nuchal muscle EMG, and behavioural arousal increased 12 h after trilostane treatment (P < 0.05). In contrast, there was no change in fetal arousal, EOG, EMG or FBM activities when progesterone was infused after the trilostane treatment. These findings show that progesterone can influence fetal behaviour, and indicates that normal progesterone production tonically suppresses arousal, or wakefulness in the fetus.