Dietary Fish Oil Alters DNA Methylation of Genes Involved in Polyunsaturated Fatty Acid Biosynthesis in Muscle and Liver of Atlantic Salmon (Salmo salar)

Adequate dietary supply of eicosapentaenoic acid (20:5n-3) and docosahexaenoic acid (22:6n-3) is required to maintain health and growth of Atlantic salmon (Salmo salar). However, salmon can also convert α-linolenic acid (18:3n-3) into eicosapentaenoic acid (20:5n-3) and docosahexaenoic acid (22:6n-3) by sequential desaturation and elongation reactions, which can be modified by 20:5n-3 and 22:6n-3 intake. In mammals, dietary 20:5n-3 + 22:6n-3 intake can modify Fads2 expression (Δ6 desaturase) via altered DNA methylation of its promoter. Decreasing dietary fish oil (FO) has been shown to increase Δ5fad expression in salmon liver. However, it is not known whether this is associated with changes in the DNA methylation of genes involved in polyunsaturated fatty acid synthesis. To address this, we investigated whether changing the proportions of dietary FO and vegetable oil altered the DNA methylation of Δ6fad_b, Δ5fad, Elovl2, and Elovl5_b promoters in liver and muscle from Atlantic salmon and whether any changes were associated with mRNA expression. Higher dietary FO content increased the proportions of 20:5n-3 and 22:6n-3 and decreased Δ6fad_b mRNA expression in liver, but there was no effect on Δ5fad, Elovl2, and Elovl5_b expression. There were significant differences between liver and skeletal muscle in the methylation of individual CpG loci in all four genes studied. Methylation of individual Δ6fad_b CpG loci was negatively related to its expression and to proportions of 20:5n-3 and 22:6n-3 in the liver. These findings suggest variations in dietary FO can induce gene-, CpG locus-, and tissue-related changes in DNA methylation in salmon.     

New piezoelectric polymers

Piezoelectricity and pyroelectricity, traditionally encountered in single crystals and ceramics, have now been documented in a number of polymers. The most successful and widely used polymer is polyvinylidene fluoride (PVF₂) which has been shown to be ferroelectric. The copolymers of PVF₂ with related fluorinated vinyl monomers also exhibit piezoelectric activity and those with trifluoroethylene (VF₃) show enhanced activity over the homopolymer. These particular copolymers crystallize into an intrinsically piezoelectric crystal phase from the melt and thus offer advantages in processing and potential application.

This paper compares piezoelectric constants obtained for commercially available PVF₂ with those for a series of PVF₂/VF₃ copolymers. One copolymer shows a 50 per cent increase in activity over PVF₂ when evaluated as a hydrophone material. The chemistry of alternative piezoelectric polymers is described. Areas of application of these exciting new materials are presented.     

The Interplay between Autonomic Nervous System and Inflammation across Systemic Autoimmune Diseases

The autonomic nervous system (ANS) and the immune system are deeply interrelated. The ANS regulates both innate and adaptive immunity through the sympathetic and parasympathetic branches, and an imbalance in this system can determine an altered inflammatory response as typically observed in chronic conditions such as systemic autoimmune diseases. Rheumatoid arthritis, systemic lupus erythematosus, and systemic sclerosis all show a dysfunction of the ANS that is mutually related to the increase in inflammation and cardiovascular risk. Moreover, an interaction between ANS and the gut microbiota has direct effects on inflammation homeostasis. Recently vagal stimulation techniques have emerged as an unprecedented possibility to reduce ANS dysfunction, especially in chronic diseases characterized by pain and a decreased quality of life as well as in chronic inflammation.     

Blood Thiol Redox State in Chronic Kidney Disease

Thiols (sulfhydryl groups) are effective antioxidants that can preserve the correct structure of proteins, and can protect cells and tissues from damage induced by oxidative stress. Abnormal levels of thiols have been measured in the blood of patients with moderate-to-severe chronic kidney disease (CKD) compared to healthy subjects, as well as in end-stage renal disease (ESRD) patients on haemodialysis or peritoneal dialysis. The levels of protein thiols (a measure of the endogenous antioxidant capacity inversely related to protein oxidation) and S-thiolated proteins (mixed disulphides of protein thiols and low molecular mass thiols), and the protein thiolation index (the molar ratio of the S-thiolated proteins to free protein thiols in plasma) have been investigated in the plasma or red blood cells of CKD and ESRD patients as possible biomarkers of oxidative stress. This type of minimally invasive analysis provides valuable information on the redox status of the less-easily accessible tissues and organs, and of the whole organism. This review provides an overview of reversible modifications in protein thiols in the setting of CKD and renal replacement therapy. The evidence suggests that protein thiols, S-thiolated proteins, and the protein thiolation index are promising biomarkers of reversible oxidative stress that could be included in the routine monitoring of CKD and ESRD patients.     

The Efficacy of Fibrinogen Concentrates in Relation to Cryoprecipitate in Restoring Clot Integrity and Stability against Lysis

Loss of fibrinogen is a feature of trauma-induced coagulopathy (TIC), and restoring this clotting factor is protective against hemorrhages. We compared the efficacy of cryoprecipitate, and of the fibrinogen concentrates RiaSTAP^® and FibCLOT^® in restoring the clot integrity in models of TIC. Cryoprecipitate and FibCLOT^® produced clots with higher maximal absorbance and enhanced resistance to lysis relative to RiaSTAP^®. The fibrin structure of clots, comprising cryoprecipitate and FibCLOT^®, mirrored those of normal plasma, whereas those with RiaSTAP^® showed stunted fibers and reduced porosity. The hemodilution of whole blood reduced the maximum clot firmness (MCF) as assessed by thromboelastography. MCF could be restored with the inclusion of 1 mg/mL of fibrinogen, but only FibCLOT^® was effective at stabilizing against lysis. The overall clot strength, measured using the Quantra^® hemostasis analyzer, was restored with both fibrinogen concentrates but not cryoprecipitate. α₂antiplasmin and plasminogen activator inhibitor-1 (PAI-1) were constituents of cryoprecipitate but were negligible in RiaSTAP^® and FibCLOT^®. Interestingly, cryoprecipitate and FibCLOT^® contained significantly higher factor XIII (FXIII) levels, approximately three-fold higher than RiaSTAP^®. Our data show that 1 mg/mL fibrinogen, a clinically achievable concentration, can restore adequate clot integrity. However, FibCLOT^®, which contained more FXIII, was superior in normalizing the clot structure and in stabilizing hemodiluted clots against mechanical and fibrinolytic degradation.     

On the Efficacy of ZnO Nanostructures against SARS-CoV-2

In 2019, the new coronavirus disease (COVID-19), related to the severe acute respiratory syndrome coronavirus (SARS-CoV-2), started spreading around the word, giving rise to the world pandemic we are still facing. Since then, many strategies for the prevention and control of COVID-19 have been studied and implemented. In addition to pharmacological treatments and vaccines, it is mandatory to ensure the cleaning and disinfection of the skin and inanimate surfaces, especially in those contexts where the contagion could spread quickly, such as hospitals and clinical laboratories, schools, transport, and public places in general. Here, we report the efficacy of ZnO nanoparticles (ZnONPs) against SARS-CoV-2. NPs were produced using an ecofriendly method and fully characterized; their antiviral activity was tested in vitro against SARS-CoV-2, showing a decrease in viral load between 70% and 90%, as a function of the material’s composition. Application of these nano-antimicrobials as coatings for commonly touched surfaces is envisaged.     

Constitutive modelling of tuff masonry in direct shear

Shear behaviour of masonry has been deeply investigated for a lot of masonry types. Nevertheless, there is still a lack of knowledge on the shear response of tuff masonry, which is widely spread in the existing building inventory in seismic areas like as Italy, Turkey, and Japan. To characterize the non-linear behaviour of the unit–mortar interface within tuff masonry, a series of direct shear tests were carried out.In this paper, both experimental results and empirical formulae are presented. Classical shear strength and deformation parameters along with shearing fracture energy and dilatancy were characterized. A shear response surface including both stress–strain diagrams and the frictional strength model is proposed.     

Identification of Human Adenovirus in Respiratory Samples with Luminex Respiratory Virus Panel Fast V2 Assay and Real-Time Polymerase Chain Reaction

In order to compare the last version of the Respiratory Virus Panel (RVP) Fast assay for human Adenovirus (hAdv) detection with a specific real-time polymerase chain reaction (qPCR), which is considered the gold standard for hAdv detection, nasopharyngeal samples collected from 309 children (age range, four months to eight years) with respiratory tract infection were tested using the RVP Fast v2 assay (Luminex Molecular Diagnostics, Inc., Toronto, ON, Canada) and a specific TaqMan qPCR to identify hAdv DNA. The RVP Fast v2 assay detected 30/61 (49.2%) hAdv infections that had been identified by real-time qPCR, demonstrating a significantly lower detection rate (p < 0.001). The sensitivity of the RVP Fast v2 assay in comparison to qPCR was lower in younger children (42.9% vs. 57.7%; Cohen’s kappa coefficient, 0.53); in samples with co-infections (40.0% vs. 56.7%; Cohen’s kappa coefficient, 0.52); and in samples with hAdv type C (45.9% vs. 57.1%; Cohen’s kappa coefficient, 0.60). Samples with lower viral loads were associated with a significantly lower sensitivity of the RVP Fast v2 assay (35.1% vs. 68.2%, p = 0.01; Cohen’s kappa coefficients, 0.49). The RVP Fast v2 assay has important limitations for the detection of hAdv and cannot be used to evaluate whether hAdvs are the main etiologic agent responsible for an outbreak or when epidemiological studies are performed.     

Energy-saving self-configuring networked data centers

In this paper, we develop the optimal minimum-energy scheduler for the dynamic online joint allocation of the task sizes, computing rates, communication rates and communication powers in virtualized Networked Data Centers (NetDCs) that operates under hard per-job delay-constraints. The referred NetDC’s infrastructure is composed by multiple frequency-scalable Virtual Machines (VMs), that are interconnected by a bandwidth and power-limited switched Local Area Network (LAN). Due to the nonlinear power-vs.-communication rate relationship, the resulting Computing-Communication Optimization Problem (CCOP) is inherently nonconvex. In order to analytically compute the exact solution of the CCOP, we develop a solving approach that relies on the following two main steps: (i) we prove that the CCOP retains a loosely coupled structure, that allows us to perform the lossless decomposition of the CCOP into the cascade of two simpler sub-problems; and, (ii) we prove that the coupling between the aforementioned sub-problems is provided by a (scalar) constraint, that is linear in the offered workload. The resulting optimal scheduler is amenable of scalable and distributed online implementation and its analytical characterization is in closed-form. After numerically testing its actual performance under randomly time-varying synthetically generated and real-world measured workload traces, we compare the obtained performance with the corresponding ones of some state-of-the-art static and sequential schedulers.     

Participatory Irrigation Management and Transfer: Setting the Guiding Principles for a Sustaining Monitoring &amp; Evaluation System – a Focus on the Mediterranean

A major shortcoming of Participatory Irrigation Management and Irrigation Management Transfer (PIM/IMT) is the absence of Monitoring & Evaluation (M&E) systems that accompany these processes and become a major instrument to correct, improve and learn. Although at the global level some countries have exerted efforts to implement M&E systems, these still present limitations, as the review of the international experiences shows. The Mediterranean situation is even more dramatic since only Tunisia implements an M&E system, whereas Egypt has shyly moved in this direction with a limited practical application. This state of affairs led the authors to develop a set of guiding principles to develop an M&E system to be applied in countries where PIM/IMT is adopted. This paper revises the main international efforts devoted to assess the performance of PIM/IMT and the state of the art of these processes in Southern and Eastern Mediterranean Countries. Moreover, it sets the framework and analyses the main sustaining factors for the development of a new M&E system for these countries.