Switchable Gratings from Polymerized Nematic Emulsions

Permanent diffraction gratings can be built in polymer dispersed liquid crystals by using either photomasks or crossed laser beams, in order to modulate the light intensity across the film during the curing process. The intensity of light diffracted from these gratings can be controlled with an applied electric field. In this paper, we report our results on the formation of permanent gratings in nematic emulsions, photopolymerized through a photomask. The gratings are permanently stored and the diffraction efficiency can be modulated with an electric field. The grating structure has been investigated by optical and scanning electron microscopy. The advantages of using nematic emulsions in photopatterning are discussed.     

Hot topic: Bisphenol A in cow milk and dietary exposure at the farm level

Chemical hazards may enter the milk chain during primary production. The study, for the first time, investigated the occurrence of bisphenol A (BPA) levels in cow milk samples collected on the farm following manual or mechanical milking and from the cooling tank. We applied a new monitoring model based on the identification of the hazards at each stage of the milk chain to identify potential pathways for contamination along the milk chain. We evaluated exposure to BPA through milk consumption based on detected contamination levels and the temporary tolerable daily intake established by the European Food Safety Authority (EFSA). Milk samples (n = 72) were analyzed using liquid chromatography with fluorescence detection. The mean BPA concentrations were 0.757 µg/L in manually milked samples, 0.580 µg/L in mechanically milked samples, and 0.797 µg/L in milk from the cooling tank. Bisphenol A occurred in the milk chain as a result of different stages of milking, and reached the highest levels at the end of the milk chain. Although the dietary intake of BPA was below the EFSA's temporary tolerable daily intake, exposure to BPA, even at low doses, through milk consumption represents a public health concern. Therefore, to ensure milk safety, new monitoring plans should be applied based on the identification of hazards at each stage of the milk chain.     

Improvement of Kiteplatin Efficacy by a Benzoato Pt(IV) Prodrug Suitable for Oral Administration

Kiteplatin, [PtCl₂(cis-1,4-DACH)] (DACH = diaminocyclohexane), contains an isomeric form of the oxaliplatin diamine ligand trans-1R,2R-DACH and has been proposed as a valuable drug candidate against cisplatin- and oxaliplatin-resistant tumors, in particular, colorectal cancer. To further improve the activity of kiteplatin, it has been transformed into a Pt(IV) prodrug by the addition of two benzoato groups in the axial positions. The new compound, cis,trans,cis-[PtCl₂(OBz)₂(cis-1,4-DACH)] (1; OBz = benzoate), showed cytotoxic activity at nanomolar concentration against a wide panel of human cancer cell lines. Based on these very promising results, the investigation has been extended to the in vivo activity of compound 1 in a Lewis Lung Carcinoma (LLC) model and its suitability for oral administration. Compound 1 resulted to be remarkably stable in acidic conditions (pH 1.5 to mimic the stomach environment) undergoing a drop of the initial concentration to ~60% of the initial one only after 72 h incubation at 37 °C; thus resulting amenable for oral administration. Interestingly, in a murine model (2·10⁶ LLC cells implanted i.m. into the right hind leg of 8-week old male and female C57BL mice), a comparable reduction of tumor mass (~75%) was observed by administering compound 1 by oral gavage and the standard drug cisplatin by intraperitoneal injection, thus indicating that, indeed, there is the possibility of oral administration for this dibenzoato prodrug of kiteplatin. Moreover, since the mechanism of action of Pt(IV) prodrugs involves an initial activation by chemical reduction to cytotoxic Pt(II) species, the reduction of 1 by two bioreductants (ascorbic acid/sodium ascorbate and glutathione) was investigated resulting to be rather slow (not complete after 120 h incubation at 37 °C). Finally, the neurotoxicity of 1 was evaluated using an in vitro assay.     

Retrospective evaluation of lead contamination in honey from 2005 to present in northeastern Italy and future perspectives in the light of updated legislation

Lead is a naturally occurring element but largely originating from human activities. Food is the major source of exposure to lead for humans and the publication of a scientific opinion from the European Food Safety Authority on the risks to human health related to the presence of lead in foodstuffs, led European Union to establish more restrictive limits for this contaminant in food from 1 January 2016. In particular, a maximum level of 0.10 mg kg^?1 was established for honey. The retrospective evaluation of 995 honey samples analysed since 2005, revealed a progressive reduction in the concentration of lead, with a mean value of 0.045 mg kg^?1 in 2015. Total 1.5% of honeys analysed in 2015 exceeded the maximum lead level and therefore will no longer be marketable. Interested beekeepers should clarify the causes of honey contamination and adopt corrective actions to keep their honey production within the legal levels of lead.     

Engineering Energy Efficient Visual Sensor Network Applications Using Skeletons

Visual sensor networks (VSNs) perform complex scene analysis algorithms that require significant computations and communications. Under this respect, the use of skeletons contributes to reduce the complexity of VSN programming and may ensure an easier and better optimization of the code. In this context, we propose INS, a stencil based skeleton targeted for wireless/visual sensor networks (W/VSNs) and give a preliminary analysis of its benefits using tracking as a case study. INS abstracts a distributed approximation schema in which the estimation of a given metric is organized in a sequence of steps. Each step includes collecting estimates from some neighbor nodes and local computation of a new approximation. In particular, INS takes inspiration from some stencil based skeletons proposed for parallel computation and merges it with the classical event driven model typical of sensor programming. As a result, the execution of each step is triggered by the detection of a relevant event in the environment. Tracking consists in periodically predicting position and velocity of one or more mobile targets. We discuss how INS can be instantiated to a distributed version of Kalman filtering. As energy efficiency is central in W/VSNs, we derive analytic models for energy dissipation of the INS skeleton depending on different concepts of neighborhood for the data exchanged at each step. Then, these models are used to guide the deployment of our tracking application on a real scenario.     

Profound retroactive interference in anterograde amnesia: What interferes?

Objective: Anterograde amnesia is characterized by a profound inability to retain new information. Recent research suggests that at least some of this severe memory impairment may be the product of retroactive interference. What exactly interferes with memory in amnesic patients, however, remains unknown. The aim of the present study was to examine whether or not postlearning material which is highly dissimilar from the material to be remembered would interfere with amnesic patients' memory. Method: Prose retention was tested in 10 densely amnesic patients and 10 controls following a 10 minute delay period, which was either unfilled (minimal interference) or filled with a tone detection task in which participants were required to listen for piano notes (nonspecific interference). Results: A significant nonspecific retroactive interference effect was observed in the amnesic patients (p     

Simulation of a functional neuromuscular stimulation powered mechanical gait orthosis with coordinated joint locking.

The purpose of this study was to examine a hybrid orthosis system (HOS) for walking after spinal-cord injury (SCI) that coordinates the mechanical locking and unlocking of knee and ankle joints of a reciprocating gait orthosis (RGO), while propulsive forces are injected and unlocked joints controlled with functional neuromuscular stimulation (FNS). The likely effectiveness of the HOS in terms of forward progression, stability, and posture of paraplegic gait was determined in this simulation study. A three-dimensional computer model of a HOS combining FNS with an RGO incorporating feedback control of muscle activation and joint locking was developed. An anthropomorphic human model included passive joint moments and a foot-ground contact model adapted from other studies. A model of the RGO reciprocally coupled the hips and locked and unlocked the knee and ankle joints during stance and swing respectively. The actions of muscles under FNS activation were modeled via closed-loop control of joint torque inputs. A walking aid that mimicked canes and voluntary upper extremity actions maintained lateral stability by providing the necessary shoulder forces and moments. The simulated HOS achieved gait speeds of 0.51 +/- 0.03 m/s, stride lengths of 0.85 +/- 0.04 m, and cadences of 72 +/- 4 steps/min, exceeding the reported performance of other assistive gait systems. Although minimal forward trunk tilt was found to be necessary during specific phases of gait, posture, and stability were significantly improved over FNS-only systems.