Integral equation method for solution of boundary value problems of structural mechanics,Part II. Elliptic partial diffeerential equations

The method presented in Part I of this paper¹ is extended to problems governed by a system of partial differential equations. The basic clarity and simplicity of the method is preserved. The domain of integration is covered by a net of orthogonal (not necessarily equidistant) straight lines. Choosing again highest derivatives with respect to each of the co-ordinates as unknowns, it is possible to reduce the problem to a system of integral equations along the lines of the adopted net. The subsequent procedure is basically the same as in Part I. The results prove to be of remarkable accuracy even for very coarse nets.     

Capturing the high-risk environment of the transition economy in Bulgaria—a simulation-based DSS

Eastern European countries have undergone a transition from centralized economic planning to more open economic systems. Hard data based upon past experience are inappropriate for decision making in this radically changed environment. A team of Bulgarian and U.S. researchers utilized system dynamics simulation to model the microeconomic environment of a Bulgarian winery expanding into regional and international markets. Expert opinion was provided for both micro- and macroeconomic factors. Given the uncertainty of the data and the ambiguity in the experts' opinions, fuzzy logic was used to model the transitional economic firm's decision making.     

Simulation of mixing effects in a VVER-1000 reactor

This work has been performed in the framework of the OECD/NEA thermalhydraulic benchmark V1000CT-2. This benchmark is related to fluid mixing in the reactor vessel during a MSLB accident scenario in a VVER-1000 reactor. Coolant mixing in a VVER-1000 V320 reactor was investigated in plant experiments during the commissioning of the Unit 6 of the Kozloduy nuclear power plant. Non-uniform and asymmetric loop flow mixing in the reactor vessel has been observed in the event of symmetric main coolant pump operation. For certain flow conditions, the experimental evidence of an azimuthal shift of the main loop flows with respect to the cold leg axes (swirl) was found.Such asymmetric flow distribution was analyzed with the Trio_U code. Trio_U is a CFD code developed by the CEA Grenoble, aimed to supply an efficient computational tool to simulate transient thermalhydraulic turbulent flows encountered in nuclear systems. For the presented study, a LES approach was used to simulate turbulent mixing. Therefore, a very precise tetrahedral mesh with more than 10 million control volumes has been created.The Trio_U calculation has correctly reproduced the measured rotation of the flow when the CAD data of the constructed reactor pressure vessel where used. This is also true for the comparison of cold leg to assembly mixing coefficients. Using the design data, the calculated swirl was significantly underestimated. Due to this result, it might be possible to improve with CFD calculations the lower plenum flow mixing matrices which are usually used in system codes.     

Personal recognition based on an image of the palmar surface of the hand

This paper describes the design and development of a multimodal biometric personal recognition system based on features extracted from a set of 14 geometrical parameters of the hand, the palmprint, four digitprints, and four fingerprints. The features are extracted from a single high-resolution gray-scale image of the palmar surface of the hand using the linear discriminant analysis (LDA) appearance-based feature-extraction approach. The information contained in the extracted features is combined at the matching-score level. The resolutions of the palmprint, digitprint and fingerprint sub-images, the similarity/dissimilarity measures, the matching-score normalization technique, and the fusion rule at the matching-score level, which optimize the system performance, were determined experimentally. The biometric system, when using a system configuration with optimum parameters, showed an average equal error rate (EER) of 0.0005%, which makes it sufficiently accurate for use in high-security biometric systems.     

Plasma treated polyethylene grafted with adhesive molecules for enhanced adhesion and growth of fibroblasts

The cell–material interface plays a crucial role in the interaction of cells with synthetic materials for biomedical use. The application of plasma for tailoring polymer surfaces is of abiding interest and holds a great promise in biomedicine. In this paper, we describe polyethylene (PE) surface tuning by Ar plasma irradiating and subsequent grafting of the chemically active PE surface with adhesive proteins or motives to support cell attachment. These simple modifications resulted in changed polymer surface hydrophilicity, roughness and morphology, which we thoroughly characterized. The effect of our modifications on adhesion and growth was tested in vitro using mouse embryonic fibroblasts (NIH 3T3 cell line). We demonstrate that the plasma treatment of PE had a positive effect on the adhesion, spreading, homogeneity of distribution and moderately on proliferation activity of NIH 3T3 cells. This effect was even more pronounced on PE coated with biomolecules.     

Fluorescence lifetime standards for time and frequency domain fluorescence spectroscopy

A series of fluorophores with single-exponential fluorescence decays in liquid solution at 20 degrees C were measured independently by nine laboratories using single-photon timing and multifrequency phase and modulation fluorometry instruments with lasers as excitation source. The dyes that can serve as fluorescence lifetime standards for time-domain and frequency-domain measurements are all commercially available, are photostable under the conditions of the measurements, and are soluble in solvents of spectroscopic quality (methanol, cyclohexane, water). These lifetime standards are anthracene, 9-cyanoanthracene, 9,10-diphenylanthracene, N-methylcarbazole, coumarin 153, erythrosin B, N-acetyl-l-tryptophanamide, 1,4-bis(5-phenyloxazol-2-yl)benzene, 2,5-diphenyloxazole, rhodamine B, rubrene, N-(3-sulfopropyl)acridinium, and 1,4-diphenylbenzene. At 20 degrees C, the fluorescence lifetimes vary from 89 ps to 31.2 ns, depending on fluorescent dye and solvent, which is a useful range for modern pico- and nanosecond time-domain or mega- to gigahertz frequency-domain instrumentation. The decay times are independent of the excitation and emission wavelengths. Dependent on the structure of the dye and the solvent, the excitation wavelengths used range from 284 to 575 nm, the emission from 330 to 630 nm. These lifetime standards may be used to either calibrate or test the resolution of time- and frequency-domain instrumentation or as reference compounds to eliminate the color effect in photomultiplier tubes. Statistical analyses by means of two-sample charts indicate that there is no laboratory bias in the lifetime determinations. Moreover, statistical tests show that there is an excellent correlation between the lifetimes estimated by the time-domain and frequency-domain fluorometries. Comprehensive tables compiling the results for 20 (fluorescence lifetime standard/solvent) combinations are given.     

Electrochemical properties of nanostructured Li1.2V3O8 in aqueous LiNO3 solution

The amorphous hydrated precursor of Li_1.2V₃O₈ was synthesized by soft chemistry method, and then heat-treated in air at several temperatures within the range 200–400 °C. The heat-treatment changed its morphological, structural and charging/discharging performance. The product obtained upon the treatment at 300 °C, consisting of uniform, rod-shaped particles, 100–150 nm in diameter and 300–800 nm in length, displayed the best electrochemical performance in aqueous LiNO₃ solution. Its initial discharge capacity amounted to 136.8 mAh g⁻¹ at a rate of C/5, which upon 50 charging/discharging cycles decreased for only 12%.     

Application of the guanidine-acylguanidine bioisosteric approach to argininamide-type NPY Y₂ receptor antagonists

Strongly basic groups such as guanidine moieties are crucial structural elements, but they compromise the drug‐likeness of numerous biologically active compounds, including ligands of G‐protein‐coupled receptors (GPCRs). As part of a project focused on the search for guanidine bioisosteres, argininamide‐type neuropeptide Y (NPY) Y₂ receptor (Y₂R) antagonists related to BIIE0246 were synthesized. Starting from ornithine derivatives, N^G‐acylated argininamides were obtained by guanidinylation with tailor‐made mono‐Boc‐protected N‐acyl‐S‐methylisothioureas. The compounds were investigated for Y₂R antagonism (calcium assays), Y₂R affinity, and NPY receptor subtype selectivity (flow cytometric binding assays). Most of the N^G‐substituted (S)‐argininamides showed Y₂R antagonistic activities and binding affinities similar to those of the parent compound, whereas N^G‐acylated or ‐carbamoylated analogues with a terminal amine were superior (Y₂R: K_i and K_B values in the low nanomolar range). This demonstrates that the basicity of the compounds, although 4–5 orders of magnitude lower than that of guanidines, is sufficient to form key interactions with acidic amino acids of the Y₂R. The acylguanidines bind with high affinity and selectivity to Y₂R over the Y₁, Y₄, and Y₅ receptors. As derivatization of the amino group is tolerated, these compounds can be considered building blocks for the preparation of versatile fluorescent and radiolabeled pharmacological tools for in vitro studies of the Y₂R. The results support the concept of bioisosteric guanidine–acylguanidine exchange as a broadly applicable approach to retain pharmacological activity despite decreased basicity.     

Micro-kinetic model of fructo-oligosaccharide synthesis for prebiotic products

A new micro-kinetic model of the enzyme-catalyzed synthesis of fructo-oligosaccharides (FOS) was developed. A commercial enzyme mixture Pectinex® Ultra SP-L derived from Aspergillus aculeatus was used. A variety of initial enzyme concentrations (1–5 vol%) and sucrose concentrations (400–600 g/L) were experimentally investigated and included in kinetic modeling. Several variations of kinetic mechanisms and corresponding models have been examined. A hybrid genetic algorithm was used to predict the kinetic parameters simultaneously for all experimental data. The best fitting model has been adopted, and with an average error of 13.34%, it describes the experimental data very well. The influence of initial concentrations on the conversion of sucrose and production of FOS is being carefully investigated. It was shown that the initial sucrose concentration significantly affects the highest level of FOS concentration, but the enzyme concentration controls the time at which maximum is reached as well as the rate of FOS decomposition.