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21.
Tajima T Takiguchi N Kato J Ikeda T Kuroda A Ohtake H 《Journal of Bioscience and Bioengineering》2003,96(2):149-153
The nematode Caenorhabditis elegans exhibits chemotaxis toward a wide variety of chemicals including water-soluble molecules and volatile organic compounds. We have previously discovered that C. elegans wild-type strain N2 is strongly attracted by cycloheximide which has long been known as a bitter tastant for humans and other mammals. We describe here the isolation and initial characterization of the first mutants which were defective specifically in their attraction to cycloheximide. In our screenings, we selected two mutants that were defective in chemotaxis to cycloheximide but normal in their attraction to NH4Cl and histidine. These mutants also avoided quinine hydrochloride, CuSO4 and high concentrations of NaCl similar to the wild-type strain N2. Furthermore, no observable defect was detected in their attraction to volatile odorants such as isoamyl alcohol and diacetyl. Dye-filling experiments suggested that they have no morphological defect in the sensory endings of the amphid neurons. 相似文献
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Pseudomonas aeruginosa as a model microorganism for investigation of chemotactic behaviors in ecosystem 总被引:1,自引:0,他引:1
Kato J Kim HE Takiguchi N Kuroda A Ohtake H 《Journal of Bioscience and Bioengineering》2008,106(1):1-7
Motile bacteria sense changes in the concentration of chemicals in environments and respond in a behavioral manner. This behavioral response is called chemotaxis. Bacterial chemotaxis can be viewed as an important prelude to metabolism, prey-predator relationships, symbiosis, infections, and other ecological interactions in biological communities. Genome analysis reveals that a large number of environmental motile bacteria possess a number of genes involved in chemosensing and chemotatic signal transduction. Pseudomonas aeruginosa has a very complex chemosensory system with more than 20 chemotaxis (che) genes in five distinct clusters and 26 chemoreceptor (methyl-accepting chemotaxis protein [mcp]) genes. Among the 26 MCPs of P. aeruginosa, nine have been identified as MCPs for amino acids, inorganic phosphate, oxygen, ethylene, and volatile chlorinated aliphatic hydrocarbons, whereas 3 MCPs were demonstrated to be involved in biofilm formation and biosynthesis of type IV pilus. Six che genes are essential for chemotactic responses, while genes in Pil-Chp cluster and Wsp cluster are involved in type IV pilus synthesis and twitching motility and biofilm formation, respectively. P. aeruginosa, with its complex chemotaxis system, is a better model microorganism for investigating ecological aspects of chemotaxis in environmental bacteria than Escherichia coli and Salmonella enterica serovar Typhimurium, which possess a relatively simpler chemotaxis system. 相似文献
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Hiroyasu Hotokezaka Noboru Aoyagi Yasuhiro Kawahara Noriko U. Yamaguchi Shinya Nagasaki Ken Sasaki Satoru Tanaka 《Microsystem Technologies》2005,11(8-10):974-979
Laser-induced breakdown spectroscopy (LIBS) has been applied for quantitative analysis of Al2O3, CaCO3 and MgO particles suspended in water. In the single elemental system, the plasma emission intensities of Al, Ca and Mg were linearly increased with concentration of elements in the range of 1.0×10?5–1.0×10?3 M, 1.0×10?4–2.0×10?3 M and 8.0×10?5 –4.0×10?3 M, respectively. We also investigated the concentration dependence of breakdown spectra for suspended mixtures of Al2O3, CaCO3 and MgO particles. The emission lines, such as Al I, Ca I, Ca II and Mg I, were appeared in the LIBS spectrum simultaneously, and each emission peak could be deconvoluted. The plasma emission intensities of Al, Ca and Mg in the multielemental system were also linearly increased with their concentrations in the range of 1.0×10?5–1.0×10?3 M, 1.0×10?4–2.0×10?3 M and 4.0×10?4–2.0×10?3 M, respectively. LIBS was found to be available for quantitative and qualitative measurement of the concentrations of Al2O3, CaCO3 and MgO particles suspended in water. The present results suggest that LIBS is a potentially useful tool for in-situ analysis on particles composition and concentrations for environmental monitoring by the wearable information equipments. 相似文献
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The method has been developed to improve the bitumen product which incorporates an evaporator concentrate from a BWR, with respect to the swelling and leaching. The leachability of the product has been measured by the method recommended by the IAEA. The swelling of the product is successfully prevented by the addition of calcium chloride. The specimen containing the waste up to Wa/B (Weight ratio of Na2SO4 + CaCl2 and bitumen) = 60:40 shows no pronounced swelling, when it is immersed in water. The cumulative fractions of 137Cs and 60Co leached from a specimen which does not contain CaCl2 are 0.65 and 0.2 at the leaching time of 30 days. On the hand, the corresponding value at 100 days for the specimen with calcium chloride addition is 5 × 10?4 for 137Cs and 1 × 10?4 for 60Co. The coating of the specimen surface with a fresh bitumen (5 mm thickness) reduces the leachability further. These results indicate that this method is effective to improve the bitumen product incorporating BWR's evaporator concentrate. 相似文献
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Learning Personal Preference From Viewer's Operations for Browsing and Its Application to Baseball Video Retrieval and Summarization 总被引:1,自引:0,他引:1
Personalization is one of the most important mechanisms to make multimedia systems easy to use. In video applications, its embodiment is to tailor video contents for a particular viewer. For this purpose, we are now developing a system of retrieving and browsing video segments, called video portal with personalization (VIPP). VIPP is characterized by 1) supporting the viewer's access to video contents and making a summarized video clip by taking his/her preference into account and 2) acquiring the viewer's profile from his/her operations automatically. In this paper, we propose a method for learning to personalize from the viewer's operations such as retrieval and browsing, as well as describe how the personalized retrieval and summarization of videos can be realized. From the experiments, we clarify the effect of personalization on retrieval and summarization of baseball videos on VIPP. 相似文献
30.
Hiroyuki Moriuchi Yoshihiro Tadokoro Masahide Sato Takeshi Furusawa Noboru Suzuki 《Journal of Electronic Materials》2007,36(3):220-225
Lead-free commercial products have been dramatically increasing in the world markets as the restriction of certain hazardous
substances (RoHS) directive in the European Union has been enacted. At the same time, the problem of “tin whiskers” has become
one of the most serious problems in the industry. Growth of tin whiskers is believed to be related to residual stress inside
the tin plating film and external compressive stress. Whiskers in fine-pitch connectors have affected the practical production
of advanced digital equipment. The Japan Electronics and Information Technology Industries Association (JEITA) has proposed
a new testing method to evaluate tin whiskers, especially for the fine-pitch connectors. This paper describes the microstructures
of external stress whiskers and the mechanical indentation test method. 相似文献